Abstract
An auxin-inducible protein kinase, VrCRK1, was isolated by a differential reverse transcriptase-polymerase chain reaction, using mRNAs extracted from auxin-treated mungbean hypocotyls. VrCRK1 exhibits high homology with plant CDPKs over catalytic domains, however, it does not have any calcium-binding EF-hand which is typically shown in plant CDPKs. Auxin treatment increased the expression level of VrCRK1. However, the increased level was reduced to basal level by treatment with PCIB, an auxin inhibitor. When extracts of mungbean hypocotyls were immunoprecipitated and the resultant immunoprecipitates were used as the enzyme source, kinase activity of VrCRK1 was found, and such activity was also increased by auxin treatment. In transgenic tobacco plants that express VrCRK1, the transcript levels of some auxin-dependent genes were elevated as much as those in wild type plants treated with auxin. These results indicate that gene expression of VrCRK1 is specifically induced by auxin, and that VrCRK1 may play a role in auxin signaling via protein phosphorylation.
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Claims 9
Claim · model-written summary
VrCRK1 was isolated from auxin-treated mungbean hypocotyls.
- Taxon
- mungbean
.jpg?width=60)
Vigna radiatae0 - Organ / stage
- hypocotylse0
- Gene
- VrCRK1e0
- Outcome
- was isolatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“VrCRK1, was isolated by a differential reverse transcriptase-polymerase chain reaction, using mRNAs extracted from auxin-treated mungbean hypocotyls”
Claim · model-written summary
VrCRK1 has high catalytic-domain homology to plant CDPKs.
Not reportedDirection
Verbatim
e0“VrCRK1 exhibits high homology with plant CDPKs over catalytic domains”
Claim · model-written summary
VrCRK1 lacks the calcium-binding EF-hand typical of CDPKs.
- Taxon
- mungbeanVigna radiatae1
- Gene
- VrCRK1e2
- Outcome
- does not have any calcium-binding EF-hande0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it does not have any calcium-binding EF-hand which is typically shown in plant CDPKs”
e1“mRNAs extracted from auxin-treated mungbean hypocotyls”
e2“VrCRK1 exhibits high homology”
Claim · model-written summary
Auxin treatment increased VrCRK1 expression.
- Taxon
- mungbeanVigna radiatae1
- Treatment
- Auxin treatmente0
- Gene
- VrCRK1e0
- Outcome
- expression level of VrCRK1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Auxin treatment increased the expression level of VrCRK1”
e1“mRNAs extracted from auxin-treated mungbean hypocotyls”
Claim · model-written summary
PCIB lowered auxin-induced VrCRK1 expression to baseline.
- Taxon
- mungbeanVigna radiatae1
- Treatment
- treatment with PCIBe0
- Gene
- VrCRK1e2
- Outcome
- increased levele0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the increased level was reduced to basal level by treatment with PCIB, an auxin inhibitor”
e1“mRNAs extracted from auxin-treated mungbean hypocotyls”
e2“expression level of VrCRK1”
Claim · model-written summary
VrCRK1 kinase activity was detected in mungbean hypocotyl immunoprecipitates.
- Taxon
- mungbeanVigna radiatae1
- Organ / stage
- hypocotylse1
- Gene
- VrCRK1e0
- Outcome
- kinase activity of VrCRK1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinase activity of VrCRK1 was found”
e1“extracts of mungbean hypocotyls were immunoprecipitated”
Claim · model-written summary
Auxin increased VrCRK1 kinase activity.
- Taxon
- mungbeanVigna radiatae1
- Treatment
- auxin treatmente0
- Gene
- VrCRK1e0
- Outcome
- kinase activity of VrCRK1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinase activity of VrCRK1 was found, and such activity was also increased by auxin treatment”
e1“mRNAs extracted from auxin-treated mungbean hypocotyls”
Claim · model-written summary
VrCRK1-transgenic tobacco elevated auxin-dependent transcripts to levels like auxin-treated wild type.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- transgenic tobacco plantse0
- Treatment
- express VrCRK1e0
- Gene
- VrCRK1e0
- Outcome
- transcript levels of some auxin-dependent genese0
- Direction
- elevatede0
- Comparator
- wild type plants treated with auxine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco plants that express VrCRK1, the transcript levels of some auxin-dependent genes were elevated as much as those in wild type plants treated with auxin”
Claim · model-written summary
The authors infer that VrCRK1 functions in auxin signaling through protein phosphorylation.
- Taxon
- mungbeanVigna radiatae1
- Gene
- VrCRK1e0
- Outcome
- auxin signalinge0
- Qualifier
- via protein phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VrCRK1 may play a role in auxin signaling via protein phosphorylation”
e1“mRNAs extracted from auxin-treated mungbean hypocotyls”
The same questions in other papers
class · molecular: expression · Vigna radiata (mung bean)Results agree
1 paper in the same direction as this paper
- positive
class · physiology: enzyme activity · Vigna radiata (mung bean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.