Abstract
CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied by real-time quantitative PCR. Additionally, stress resistance, such as the freezing resistance of CsICE1, was studied using Arabidopsis lines transformed with sense or anti-sense CsICE1 via Agrobacterium tumefaciens infection. Our results showed that CsICE1 mRNA could be induced under -5°C, PEG, ABA, or BR treatment, although the pattern of expression differed for all treatments. Compared to wild type (WT) and anti-sense ICE1 transgenic lines, sense lines displayed higher relative germination rates under salt and drought stress. After freezing treatment, the sense transgenic lines over-expressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde. Conversely, compared with WT, anti-sense ICE1 transgenic lines had lower proline levels and higher malonaldehyde levels under freezing conditions. Our study indicates that CsICE1 is an important anti-freezing gene and that over-expression of CsICE1 can improve cold resistance and enhance salt and drought tolerance of transgenic lines.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
CsICE1 mRNA was induced by -5°C, PEG, ABA and BR, with treatment-specific expression patterns.
- Taxon
- tea plantse1
- Treatment
- -5°C, PEG, ABA, or BR treatmente0
- Gene
- CsICE1e0
- Outcome
- CsICE1 mRNAe0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“CsICE1 mRNA could be induced under -5°C, PEG, ABA, or BR treatment, although the pattern of expression differed for all treatments”
e1“tea plants”
Claim · model-written summary
Sense CsICE1 Arabidopsis lines had higher relative germination under salt and drought stress than wild type and antisense lines.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- sense linese0
- Treatment
- salt and drought stresse0
- Outcome
- relative germination ratese0
- Direction
- highere0
- Comparator
- wild type (WT) and anti-sense ICE1 transgenic linese0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild type (WT) and anti-sense ICE1 transgenic lines, sense lines displayed higher relative germination rates under salt and drought stress”
t0“transgenic Arabidopsis”
Claim · model-written summary
After freezing, sense CsICE1 lines had higher survival, increased proline and decreased malonaldehyde.
- Taxon
- ArabidopsisArabidopsist0
- Material
- sense transgenic linese0
- Treatment
- freezing treatmente0
- Gene
- CsICE1e0
- Outcome
- survival ratee0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After freezing treatment, the sense transgenic lines over-expressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde”
t0“transgenic Arabidopsis”
Claim · model-written summary
After freezing, sense CsICE1 lines had increased proline levels.
- Taxon
- ArabidopsisArabidopsist0
- Material
- sense transgenic linese0
- Treatment
- freezing treatmente0
- Gene
- CsICE1e0
- Outcome
- levels of prolinee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After freezing treatment, the sense transgenic lines over-expressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde”
t0“transgenic Arabidopsis”
Claim · model-written summary
After freezing, sense CsICE1 lines had decreased malonaldehyde levels.
- Taxon
- ArabidopsisArabidopsist0
- Material
- sense transgenic linese0
- Treatment
- freezing treatmente0
- Gene
- CsICE1e0
- Outcome
- levels of malonaldehydee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After freezing treatment, the sense transgenic lines over-expressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde”
t0“transgenic Arabidopsis”
Claim · model-written summary
Antisense ICE1 lines had lower proline levels than wild type under freezing.
- Taxon
- ArabidopsisArabidopsist0
- Material
- anti-sense ICE1 transgenic linese0
- Treatment
- freezing conditionse0
- Gene
- ICE1e0
- Outcome
- proline levelse0
- Direction
- lowere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared with WT, anti-sense ICE1 transgenic lines had lower proline levels and higher malonaldehyde levels under freezing conditions”
t0“transgenic Arabidopsis”
Claim · model-written summary
Antisense ICE1 lines had higher malonaldehyde levels than wild type under freezing.
- Taxon
- ArabidopsisArabidopsist0
- Material
- anti-sense ICE1 transgenic linese0
- Treatment
- freezing conditionse0
- Gene
- ICE1e0
- Outcome
- malonaldehyde levelse0
- Direction
- highere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared with WT, anti-sense ICE1 transgenic lines had lower proline levels and higher malonaldehyde levels under freezing conditions”
t0“transgenic Arabidopsis”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Camellia sinensis (black tea)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
3 papers more in this group
See all 7 papers →brassinolide · molecular: expression · Camellia sinensis (black tea)Results agree
1 paper in the same direction as this paper
- positive
cold · molecular: metabolites · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
6 papers more in this group
See all 10 papers →drought · molecular: expression · Camellia sinensis (black tea)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.