Paper · 1999 · HortScience

Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro

Kenneth R. Schroeder; Dennis P. Stimart

5
claims
10
verbatim quotes
3
citations
3
research areas
✓
Open access

Abstract

Hypocotyls from Antirrhinum majus L. were excised at 2 weeks of age from seedlings grown under a 16-hour photoperiod or continuous darkness. Explants were cultured on modified Murashige-Skoog (MS) medium containing 0, 0.44, 2.22, 4.44, 8.88, or 44.4 μ m BA to investigate adventitious shoot formation. Excised hypocotyls from eight commercial cultivars, three inbred lines, and an F 1 hybrid between two of the inbreds were cultured on MS medium containing 2.22 μ m BA to assess genotypic effects on adventitious shoot formation. The influence of seedling age was assessed by excising hypocotyls from seedlings at 6, 10, 14, 18, 22, 26, or 30 days. Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings, and culture on medium containing 2.22 μ m BA for 3 weeks. Under these conditions, up to a 5-fold improvement in number of shoots per hypocotyl over previous studies was achieved. Adventitious shoot formation was genotype-dependent and appeared to be a dominant trait. Chemical name used: N 6 -benzyladenine (BA).

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Light-grown seedlings provided optimal hypocotyl shoot formation.

Taxon
Antirrhinum majus L.Antirrhinum majust0
Treatment
seedling growth in a lighted environmente0
Outcome
adventitious shoot formatione0
Direction
Optimale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Antirrhinum majus
Antirrhinum majus · TaxonUser:Haplochromis · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment”

✓ located verbatim in the source · abstract, characters 649–773 · show in text ↑
t0

“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”

✓ located verbatim in the source · title, characters 0–96 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Hypocotyls from 10-day-old seedlings provided optimal shoot formation.

Taxon
Antirrhinum majus L.Antirrhinum majust0
Treatment
use of hypocotyls from 10-day-old seedlingse0
Outcome
adventitious shoot formatione0
Direction
Optimale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Antirrhinum majus
Antirrhinum majus · TaxonUser:Haplochromis · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings”

✓ located verbatim in the source · abstract, characters 649–818 · show in text ↑
t0

“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”

✓ located verbatim in the source · title, characters 0–96 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Culture with 2.22 μ m BA for three weeks provided optimal shoot formation.

Taxon
Antirrhinum majus L.Antirrhinum majust0
Treatment
culture on medium containing 2.22 μ m BA for 3 weekse0
Outcome
adventitious shoot formatione0
Direction
Optimale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Antirrhinum majus
Antirrhinum majus · TaxonUser:Haplochromis · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings, and culture on medium containing 2.22 μ m BA for 3 weeks”

✓ located verbatim in the source · abstract, characters 649–876 · show in text ↑
t0

“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”

✓ located verbatim in the source · title, characters 0–96 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The optimized conditions yielded up to fivefold more shoots per hypocotyl than previous studies.

Taxon
Antirrhinum majus L.Antirrhinum majust0
Treatment
these conditionse0
Outcome
number of shoots per hypocotyle0
Direction
improvemente0
Value
5-folde0
Comparator
previous studiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Antirrhinum majus
Antirrhinum majus · TaxonUser:Haplochromis · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Under these conditions, up to a 5-fold improvement in number of shoots per hypocotyl over previous studies was achieved”

✓ located verbatim in the source · abstract, characters 878–997 · show in text ↑
t0

“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”

✓ located verbatim in the source · title, characters 0–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Adventitious shoot formation depended on genotype and appeared dominant.

Taxon
Antirrhinum majus L.Antirrhinum majust0
Outcome
Adventitious shoot formatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Antirrhinum majus
Antirrhinum majus · TaxonUser:Haplochromis · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Adventitious shoot formation was genotype-dependent and appeared to be a dominant trait”

✓ located verbatim in the source · abstract, characters 999–1086 · show in text ↑
t0

“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”

✓ located verbatim in the source · title, characters 0–96 · show in text ↑

N-benzyladenine · growth · Antirrhinum majus (snapdragon)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

in vitro · growth · Antirrhinum majus (snapdragon)Positive and negative results

6 papers: 5 positive, 1 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

light · growth · Antirrhinum majus (snapdragon)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.