Abstract
Hypocotyls from Antirrhinum majus L. were excised at 2 weeks of age from seedlings grown under a 16-hour photoperiod or continuous darkness. Explants were cultured on modified Murashige-Skoog (MS) medium containing 0, 0.44, 2.22, 4.44, 8.88, or 44.4 μ m BA to investigate adventitious shoot formation. Excised hypocotyls from eight commercial cultivars, three inbred lines, and an F 1 hybrid between two of the inbreds were cultured on MS medium containing 2.22 μ m BA to assess genotypic effects on adventitious shoot formation. The influence of seedling age was assessed by excising hypocotyls from seedlings at 6, 10, 14, 18, 22, 26, or 30 days. Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings, and culture on medium containing 2.22 μ m BA for 3 weeks. Under these conditions, up to a 5-fold improvement in number of shoots per hypocotyl over previous studies was achieved. Adventitious shoot formation was genotype-dependent and appeared to be a dominant trait. Chemical name used: N 6 -benzyladenine (BA).
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Claims 5
Claim · model-written summary
Light-grown seedlings provided optimal hypocotyl shoot formation.
- Taxon
- Antirrhinum majus L.
Antirrhinum majust0
- Treatment
- seedling growth in a lighted environmente0
- Outcome
- adventitious shoot formatione0
- Direction
- Optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment”
t0“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”
Claim · model-written summary
Hypocotyls from 10-day-old seedlings provided optimal shoot formation.
- Taxon
- Antirrhinum majus L.
Antirrhinum majust0
- Treatment
- use of hypocotyls from 10-day-old seedlingse0
- Outcome
- adventitious shoot formatione0
- Direction
- Optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings”
t0“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”
Claim · model-written summary
Culture with 2.22 μ m BA for three weeks provided optimal shoot formation.
- Taxon
- Antirrhinum majus L.
Antirrhinum majust0
- Treatment
- culture on medium containing 2.22 μ m BA for 3 weekse0
- Outcome
- adventitious shoot formatione0
- Direction
- Optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal conditions for adventitious shoot formation on excised hypocotyls included: seedling growth in a lighted environment, use of hypocotyls from 10-day-old seedlings, and culture on medium containing 2.22 μ m BA for 3 weeks”
t0“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”
Claim · model-written summary
The optimized conditions yielded up to fivefold more shoots per hypocotyl than previous studies.
- Taxon
- Antirrhinum majus L.
Antirrhinum majust0
- Treatment
- these conditionse0
- Outcome
- number of shoots per hypocotyle0
- Direction
- improvemente0
- Value
- 5-folde0
- Comparator
- previous studiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under these conditions, up to a 5-fold improvement in number of shoots per hypocotyl over previous studies was achieved”
t0“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”
Claim · model-written summary
Adventitious shoot formation depended on genotype and appeared dominant.
- Taxon
- Antirrhinum majus L.
Antirrhinum majust0
- Outcome
- Adventitious shoot formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Adventitious shoot formation was genotype-dependent and appeared to be a dominant trait”
t0“Adventitious Shoot Formation on Excised Hypocotyls of Antirrhinum majus L. (Snapdragon) In Vitro”
The same questions in other papers
N-benzyladenine · growth · Antirrhinum majus (snapdragon)Results agree
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- positive
in vitro · growth · Antirrhinum majus (snapdragon)Positive and negative results
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3 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative
- no effect
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.