Abstract
Diclofop-methyl (methyl ester of 2-[4-(2',4'-dichlorophenoxy)phenoxy]propionate; 100 micromolar) and diclofop (100 micromolar) inhibited both ATP- and PPi-dependent formation of H+ gradients by tonoplast vesicles isolated from oat (Avena sativa L., cv Dal) roots. Diclofop-methyl (1 micromolar) significantly reduced the steady-state H+ gradient generated in the presence of ATP. The ester (diclofop-methyl) was more inhibitory than the free acid (diclofop) at pH 7.4, but this relative activity was reversed at pH 5.7. Neither compound affected the rate of ATP or PPi hydrolysis by the proton-pumping enzymes. Diclofop-methyl (50, 100 micromolar), but not diclofop (100 micromolar), accelerated the decay of nonmetabolic H+ gradients established across vesicle membranes. Diclofop-methyl (100 micromolar) did not collapse K+ gradients across vesicle membranes. Both the (+)and (-)-enantiomers of diclofop-methyl dissipated nonmetabolic H+ gradients established across vesicle membranes. Diclofopmethyl, but not diclofop (each 100 micromolar), accelerated the decay of H+ gradients imposed across liposomal membranes. These results show that diclofop-methyl causes a specific increase in the H+ permeability of tonoplast.
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Claims 10
Claim · model-written summary
Diclofop-methyl and diclofop inhibited ATP- and PPi-dependent H+ gradient formation in oat root tonoplast vesicles.
- Taxon
- oat
Avena sativae0 - Material
- cv Dale0
- Organ / stage
- rootse0
- Treatment
- Diclofop-methyle0
- Outcome
- ATP- and PPi-dependent formation of H+ gradientse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diclofop-methyl (methyl ester of 2-[4-(2',4'-dichlorophenoxy)phenoxy]propionate; 100 micromolar) and diclofop (100 micromolar) inhibited both ATP- and PPi-dependent formation of H+ gradients by tonoplast vesicles isolated from oat (Avena sativa L., cv Dal) roots.”
Claim · model-written summary
Diclofop-methyl at 1 micromolar significantly reduced the ATP-generated steady-state H+ gradient.
- Taxon
- OatAvena sativat0
- Treatment
- Diclofop-methyl (1 micromolar)e0
- Outcome
- steady-state H+ gradiente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diclofop-methyl (1 micromolar) significantly reduced the steady-state H+ gradient generated in the presence of ATP.”
t0“Oat-Root”
Claim · model-written summary
Diclofop-methyl was more inhibitory than diclofop at pH 7.4.
- Taxon
- OatAvena sativat0
- Treatment
- diclofop-methyle0
- Outcome
- more inhibitorye0
- Direction
- more inhibitorye0
- Qualifier
- pH 7.4e0
- Comparator
- diclofope0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ester (diclofop-methyl) was more inhibitory than the free acid (diclofop) at pH 7.4”
t0“Oat-Root”
Claim · model-written summary
Diclofop was more inhibitory than diclofop-methyl at pH 5.7.
- Taxon
- OatAvena sativat0
- Treatment
- diclofope0
- Outcome
- relative activitye0
- Direction
- reversede0
- Qualifier
- pH 5.7e0
- Comparator
- diclofop-methyle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ester (diclofop-methyl) was more inhibitory than the free acid (diclofop) at pH 7.4, but this relative activity was reversed at pH 5.7.”
t0“Oat-Root”
Claim · model-written summary
Neither compound affected ATP or PPi hydrolysis rate.
- Taxon
- OatAvena sativat0
- Treatment
- compounde0
- Outcome
- rate of ATP or PPi hydrolysise0
- Direction
- Neither compound affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither compound affected the rate of ATP or PPi hydrolysis by the proton-pumping enzymes.”
t0“Oat-Root”
Claim · model-written summary
Diclofop-methyl accelerated decay of nonmetabolic H+ gradients across vesicle membranes.
- Taxon
- OatAvena sativat0
- Treatment
- Diclofop-methyl (50, 100 micromolar)e0
- Outcome
- decay of nonmetabolic H+ gradientse0
- Direction
- acceleratede0
- Comparator
- diclofop (100 micromolar)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diclofop-methyl (50, 100 micromolar), but not diclofop (100 micromolar), accelerated the decay of nonmetabolic H+ gradients established across vesicle membranes.”
t0“Oat-Root”
Claim · model-written summary
Diclofop-methyl did not collapse K+ gradients across vesicle membranes.
- Taxon
- OatAvena sativat0
- Treatment
- Diclofop-methyl (100 micromolar)e0
- Outcome
- K+ gradientse0
- Direction
- did not collapsee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diclofop-methyl (100 micromolar) did not collapse K+ gradients across vesicle membranes.”
t0“Oat-Root”
Claim · model-written summary
Both diclofop-methyl enantiomers dissipated nonmetabolic H+ gradients.
- Taxon
- OatAvena sativat0
- Treatment
- diclofop-methyle0
- Outcome
- nonmetabolic H+ gradientse0
- Direction
- dissipatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the (+)and (-)-enantiomers of diclofop-methyl dissipated nonmetabolic H+ gradients established across vesicle membranes.”
t0“Oat-Root”
Claim · model-written summary
Diclofop-methyl accelerated decay of H+ gradients across liposomal membranes while diclofop did not.
- Taxon
- OatAvena sativat0
- Treatment
- Diclofopmethyle0
- Outcome
- decay of H+ gradientse0
- Direction
- acceleratede0
- Comparator
- diclofope0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diclofopmethyl, but not diclofop (each 100 micromolar), accelerated the decay of H+ gradients imposed across liposomal membranes.”
t0“Oat-Root”
Claim · model-written summary
Diclofop-methyl specifically increases tonoplast H+ permeability.
- Taxon
- OatAvena sativat0
- Treatment
- diclofop-methyle0
- Outcome
- H+ permeability of tonoplaste0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results show that diclofop-methyl causes a specific increase in the H+ permeability of tonoplast.”
t0“Oat-Root”
The same questions in other papers
diclofop-methyl · disease · Avena sativa (oats)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
diclofop-methyl · molecular: metabolites · Avena sativa (oats)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.