Paper · 1999 · Chinese Journal of Appplied Environmental Biology

EFFECT OF EXOGENOUS H 2O 2 ON THE ANTIOXIDANT SYSTEM IN CALLI OF TWO MAIZE CULTIVARS WITH DIFFERENT DROUGHT RESISTANCE

Li Ling

7
claims
14
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

The effect of exogenous H 2O 2 on antioxidant system was studied in callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.). Eight day old calli were harvested and soaked with hydrogen peroxide solution (10 -4 , 10 -3 and 10 -2 mol L -1 ) for 3 h. Hydrogen peroxide damaged the calli, as indicated by the increase in leakage of electrolytes, the contents of H 2O 2 and malondiadehyde, as well as the reduction in ascorbate and carotenoid contents in the calli of the both cultivars. Hydrogen peroxide (10 -4 mol/L ) had small oxidant effect. When calli were treated by hydrogen peroxide, the activities of SOD, POD, AP and GR, and the levels of AsA and CAR remained higher in the calli of the drought resistant cultivar than those of the drought sensitive one. The results showed that the higher activity of the antioxidant system had a greater protective effect against oxidative stress caused by hydrogen peroxide in drough resistant cultivar than in the drought sensitive cultivar.

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Claims 7

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Hydrogen peroxide increased electrolyte leakage and H2O2 and malondialdehyde contents in both maize callus cultivars.

Taxon
maizeZea mayse0
Material
drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivarse0
Organ / stage
callus tissuese0
Treatment
Hydrogen peroxidee1
Outcome
leakage of electrolytes, the contents of H 2O 2 and malondiadehydee1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e1

“Hydrogen peroxide damaged the calli, as indicated by the increase in leakage of electrolytes, the contents of H 2O 2 and malondiadehyde, as well as the reduction in ascorbate and carotenoid contents in the calli of the both cultivars”

✓ located verbatim in the source · abstract, characters 304–537 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Hydrogen peroxide reduced ascorbate and carotenoid contents in both maize callus cultivars.

Taxon
maizeZea mayse0
Material
drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivarse0
Organ / stage
callus tissuese0
Treatment
Hydrogen peroxidee1
Outcome
ascorbate and carotenoid contentse1
Direction
reductione1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e1

“Hydrogen peroxide damaged the calli, as indicated by the increase in leakage of electrolytes, the contents of H 2O 2 and malondiadehyde, as well as the reduction in ascorbate and carotenoid contents in the calli of the both cultivars”

✓ located verbatim in the source · abstract, characters 304–537 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hydrogen peroxide at 10^-4 mol/L had a small oxidant effect on maize callus.

Taxon
maizeZea mayse0
Treatment
Hydrogen peroxide (10 -4 mol/L )e2
Outcome
oxidant effecte2
Qualifier
smalle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e2

“Hydrogen peroxide (10 -4 mol/L ) had small oxidant effect”

✓ located verbatim in the source · abstract, characters 539–596 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under hydrogen peroxide, SOD, POD and AP activities were higher in the drought-resistant maize callus than in the drought-sensitive one.

Taxon
maizeZea mayse0
Material
PAN 6043e0
Organ / stage
callie4
Treatment
hydrogen peroxidee4
Outcome
activities of SOD, POD, APe4
Direction
highere4
Comparator
those of the drought sensitive onee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e4

“When calli were treated by hydrogen peroxide, the activities of SOD, POD, AP and GR, and the levels of AsA and CAR remained higher in the calli of the drought resistant cultivar than those of the drought sensitive one”

✓ located verbatim in the source · abstract, characters 598–815 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under hydrogen peroxide, GR activity was higher in the drought-resistant maize callus than in the drought-sensitive one.

Taxon
maizeZea mayse0
Material
PAN 6043e0
Organ / stage
callie4
Treatment
hydrogen peroxidee4
Outcome
GRe4
Direction
highere4
Comparator
those of the drought sensitive onee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e4

“When calli were treated by hydrogen peroxide, the activities of SOD, POD, AP and GR, and the levels of AsA and CAR remained higher in the calli of the drought resistant cultivar than those of the drought sensitive one”

✓ located verbatim in the source · abstract, characters 598–815 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under hydrogen peroxide, AsA and CAR levels were higher in the drought-resistant maize callus than in the drought-sensitive one.

Taxon
maizeZea mayse0
Material
PAN 6043e0
Organ / stage
callie4
Treatment
hydrogen peroxidee4
Outcome
the levels of AsA and CARe4
Direction
highere4
Comparator
those of the drought sensitive onee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e4

“When calli were treated by hydrogen peroxide, the activities of SOD, POD, AP and GR, and the levels of AsA and CAR remained higher in the calli of the drought resistant cultivar than those of the drought sensitive one”

✓ located verbatim in the source · abstract, characters 598–815 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Higher antioxidant-system activity gave a greater protective effect against hydrogen peroxide-induced oxidative stress in the drought-resistant cultivar.

Taxon
maizeZea mayse0
Outcome
greater protective effect against oxidative stresse7
Qualifier
higher activity of the antioxidant systeme7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“callus tissues of drought-resistant (PAN 6043) and drought-sensitive (SC 701) cultivars of maize ( Zea mays L.)”

✓ located verbatim in the source · abstract, characters 68–179 · show in text ↑
e7

“The results showed that the higher activity of the antioxidant system had a greater protective effect against oxidative stress caused by hydrogen peroxide in drough resistant cultivar than in the drought sensitive cultivar”

✓ located verbatim in the source · abstract, characters 817–1039 · show in text ↑

hydrogen peroxide · physiology: oxidative · Zea mays (maize)Results differ

8 papers: 7 positive, 1 negative or no effect

7 in the opposite direction

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

hydrogen peroxide · physiology: pigments · Zea mays (maize)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

hydrogen peroxide · molecular: metabolites · Zea mays (maize)Positive and negative results

8 papers: 6 positive, 2 negative or no effect

5 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

4 papers more in this group

See all 8 papers →

hydrogen peroxide · physiology: antioxidant enzymes · Zea mays (maize)Positive and negative results

8 papers: 7 positive, 1 negative or no effect

6 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.