Abstract
In this study, cucumber seedlings were cultivated in nutrient solution added with different concentration (0.05, 0.1, 0.2, and 0.3 mmol x L(-1)) sodium nitroprusside (SNP) as nitric oxide (NO) donor to study the effects of exogenous NO on the growth of cucumber seedlings and the activities of antioxidant enzymes in cucumber leaves under NO3- stress. Under the stress of 140 mmol x L(-1) NO3-, treating with 0.1 mmol x L(-1) of SNP for 1 d or 7 d increased the leaf soluble protein content and superoxidase (SOD), catalase (CAT), and ascorbic acid peroxidase (APX) activities, and decreased the leaf malondialdehyde (MDA) content significantly, suggesting that exogenous NO could enhance the capacity of cucumber seedlings in scavenging active oxygen species, protect the seedlings from the peroxidation of membrane lipids, and promote the seedlings growth and increase their resistance to high concentration NO3- stress. After the cucumber seedlings grew in 0.3 mmol x L(-1) of SNP for 7 d, the activities of leaf SOD, POD, and CAT decreased, and the MDA content increased, resulting in the injury of cucumber seedlings. It was indicated that certain concentration (0.1-0.2 mmol x L(-1)) exogenous NO could alleviate the NO3- stress to cucumber seedlings.
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Claims 10
Claim · model-written summary
At 0.1 mmol x L(-1) SNP under nitrate stress, leaf soluble protein increased.
- Taxon
- cucumber seedlings
Cucumis sativust0
- Treatment
- 0.1 mmol x L(-1) of SNP for 1 d or 7 de0
- Outcome
- leaf soluble protein contente0
- Direction
- increasede0
- Qualifier
- 140 mmol x L(-1) NO3-e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under the stress of 140 mmol x L(-1) NO3-, treating with 0.1 mmol x L(-1) of SNP for 1 d or 7 d increased the leaf soluble protein content”
t0“cucumber seedlings”
Claim · model-written summary
The 0.1 mmol x L(-1) SNP treatment increased leaf SOD, CAT and APX activities under nitrate stress.
- Taxon
- cucumber seedlings
Cucumis sativust0
- Treatment
- 0.1 mmol x L(-1) of SNP for 1 d or 7 de0
- Outcome
- superoxidase (SOD), catalase (CAT), and ascorbic acid peroxidase (APX) activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treating with 0.1 mmol x L(-1) of SNP for 1 d or 7 d increased the leaf soluble protein content and superoxidase (SOD), catalase (CAT), and ascorbic acid peroxidase (APX) activities”
t0“cucumber seedlings”
Claim · model-written summary
The 0.1 mmol x L(-1) SNP treatment significantly decreased leaf MDA content under nitrate stress.
- Taxon
- cucumber seedlings
Cucumis sativust0
- Outcome
- leaf malondialdehyde (MDA) contente0
- Direction
- decreasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased the leaf malondialdehyde (MDA) content significantly”
t0“cucumber seedlings”
Claim · model-written summary
The authors infer that exogenous NO enhances active oxygen scavenging in cucumber seedlings.
- Taxon
- cucumber seedlings
Cucumis sativuse0
- Treatment
- exogenous NOe0
- Outcome
- capacity of cucumber seedlings in scavenging active oxygen speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous NO could enhance the capacity of cucumber seedlings in scavenging active oxygen species”
Claim · model-written summary
The authors infer that exogenous NO protects seedlings from membrane lipid peroxidation.
- Taxon
- cucumber seedlings
Cucumis sativust0
- Outcome
- peroxidation of membrane lipidse0
- Qualifier
- protect the seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protect the seedlings from the peroxidation of membrane lipids”
t0“cucumber seedlings”
Claim · model-written summary
The authors infer that exogenous NO promotes cucumber growth and nitrate-stress resistance.
- Taxon
- cucumber seedlings
Cucumis sativust0
- Outcome
- seedlings growth and increase their resistance to high concentration NO3- stresse0
- Qualifier
- high concentration NO3- stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promote the seedlings growth and increase their resistance to high concentration NO3- stress”
t0“cucumber seedlings”
Claim · model-written summary
At 0.3 mmol x L(-1) SNP for seven days, leaf SOD, POD and CAT activities decreased.
- Taxon
- cucumber seedlings
Cucumis sativuse0
- Treatment
- 0.3 mmol x L(-1) of SNP for 7 de0
- Outcome
- activities of leaf SOD, POD, and CATe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After the cucumber seedlings grew in 0.3 mmol x L(-1) of SNP for 7 d, the activities of leaf SOD, POD, and CAT decreased”
Claim · model-written summary
At 0.3 mmol x L(-1) SNP for seven days, leaf MDA content increased.
- Taxon
- cucumber seedlings
Cucumis sativuse0
- Treatment
- 0.3 mmol x L(-1) of SNP for 7 de0
- Outcome
- MDA contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After the cucumber seedlings grew in 0.3 mmol x L(-1) of SNP for 7 d, the activities of leaf SOD, POD, and CAT decreased, and the MDA content increased”
Claim · model-written summary
The high SNP concentration injured cucumber seedlings.
- Taxon
- cucumber seedlings
Cucumis sativuse0
- Outcome
- injury of cucumber seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resulting in the injury of cucumber seedlings”
Claim · model-written summary
The authors conclude that 0.1–0.2 mmol x L(-1) exogenous NO alleviates nitrate stress in cucumber seedlings.
- Taxon
- cucumber seedlings
Cucumis sativuse0
- Treatment
- 0.1-0.2 mmol x L(-1)) exogenous NOe0
- Outcome
- NO3- stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“certain concentration (0.1-0.2 mmol x L(-1)) exogenous NO could alleviate the NO3- stress to cucumber seedlings”
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