Abstract
To establish the most optimal callus subculture system on YTB mature embryo,the effect of different callus subculture conditions on YTB mature embryo were studied.The results showed that NB medium was more suitable to the growth of callus subculture; YTB callus from mature embryos wasn't suitable for the second subculture.Fe iron concentration had a significant impact on callus subculture;hydrolysis of casein protein,proline,glutamine and sodium thiosulfate improved the quality of callus and reduced the browning rate;ascorbic acid(VC) had no significant impact on the embryo callus subculture growth and anti-browning.The consistency of morphology and cytology observation proved that exogenous putrescine(30 mg/L) in the callus subculture maintained callus in a good embryogenic condition,thereby enhanced the subsequent genetic transformation rate.
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Claims 8
Claim · model-written summary
NB medium was more suitable for the growth of rice callus subculture.
- Taxon
- indica rice
Oryza sativa Indica Groupt0 - Treatment
- NB mediume1
- Outcome
- growth of callus subculturee1
- Direction
- more suitablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“NB medium was more suitable to the growth of callus subculture”
Claim · model-written summary
Callus from YTB mature embryos was not suitable for a second subculture.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Organ / stage
- mature embryose1
- Outcome
- suitable for the second subculturee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“YTB callus from mature embryos wasn't suitable for the second subculture”
Claim · model-written summary
Fe iron concentration had a significant impact on rice callus subculture.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- Fe iron concentratione1
- Outcome
- callus subculturee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“Fe iron concentration had a significant impact on callus subculture”
Claim · model-written summary
Hydrolysed casein protein, proline, glutamine and sodium thiosulfate improved the quality of rice callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- hydrolysis of casein protein,proline,glutamine and sodium thiosulfatee1
- Outcome
- quality of calluse1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“hydrolysis of casein protein,proline,glutamine and sodium thiosulfate improved the quality of callus and reduced the browning rate”
Claim · model-written summary
Hydrolysed casein protein, proline, glutamine and sodium thiosulfate reduced the browning rate of rice callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- hydrolysis of casein protein,proline,glutamine and sodium thiosulfatee1
- Outcome
- browning ratee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“hydrolysis of casein protein,proline,glutamine and sodium thiosulfate improved the quality of callus and reduced the browning rate”
Claim · model-written summary
Ascorbic acid (VC) had no significant impact on rice embryo callus subculture growth or anti-browning.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- ascorbic acid(VC)e1
- Outcome
- embryo callus subculture growth and anti-browninge1
- Direction
- no significant impacte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“ascorbic acid(VC) had no significant impact on the embryo callus subculture growth and anti-browning”
Claim · model-written summary
Exogenous putrescine (30 mg/L) in callus subculture maintained rice callus in a good embryogenic condition.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- exogenous putrescine(30 mg/L)e1
- Outcome
- embryogenic conditione1
- Direction
- maintainede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“exogenous putrescine(30 mg/L) in the callus subculture maintained callus in a good embryogenic condition”
Claim · model-written summary
Exogenous putrescine (30 mg/L) in callus subculture enhanced the subsequent genetic transformation rate of rice callus.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- exogenous putrescine(30 mg/L)e1
- Outcome
- genetic transformation ratee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“indica rice callus”
e1“exogenous putrescine(30 mg/L) in the callus subculture maintained callus in a good embryogenic condition,thereby enhanced the subsequent genetic transformation rate”
The same questions in other papers
ascorbic acid · growth · Oryza sativa (rice)Results differ
1 paper in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →glutamine · disease · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
proline · quality: general · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
glutamine · quality: general · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
proline · disease · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Oryza sativa (rice)Positive and negative results
139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
161 papers more in this group
See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.