Abstract
The full-length cDNA of Catalase 1(CAT1) from Nicotiana tabacum var. NC89 was cloned based on specific primers designed according to CAT1 mRNA sequences in other plants. An amino acid sequence alignment indicated that CAT1 contains 492 amino acid residues. Phylogenetic analysis showed that NC89 CAT1 shares high similarity with gene from N. benthamiana. Real-time quantitative polymerase chain reaction(qPCR) analysis revealed that CAT1 is highly expressed in leaf and calyx, while less expressed in roots and seeds. The expression of NC89 CAT1 was enhanced by abiotic and biotic stressors, such as mechanical damage, osmotic pressure, low temperature, high temperature, drought, and TMV infection, whereas it was inhibited by ultraviolet irradiation. It was suggested that CAT1 plays an important role in growth, development, and interplay of abiotic and biotic stresses in plant.
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Claims 6
Claim · model-written summary
The full-length CAT1 cDNA was cloned from tobacco.
- Taxon
- Nicotiana tabacum var. NC89
Nicotiana tabacume0 - Gene
- CAT1e0
- Outcome
- full-length cDNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full-length cDNA of Catalase 1(CAT1) from Nicotiana tabacum var. NC89 was cloned”
t0“Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L”
Claim · model-written summary
CAT1 encodes 492 amino acid residues.
- Taxon
- Nicotiana tabacum LNicotiana tabacumt0
- Gene
- CAT1e0
- Outcome
- amino acid residuese0
- Value
- 492 amino acid residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CAT1 contains 492 amino acid residues”
t0“Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L”
Claim · model-written summary
NC89 CAT1 showed high similarity to a N. benthamiana gene.
- Taxon
- Nicotiana tabacum LNicotiana tabacumt0
- Gene
- NC89 CAT1e0
- Outcome
- similaritye0
- Direction
- highe0
- Comparator
- gene from N. benthamianae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NC89 CAT1 shares high similarity with gene from N. benthamiana”
t0“Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L”
Claim · model-written summary
CAT1 expression was higher in leaf and calyx than in roots and seeds.
- Taxon
- Nicotiana tabacum LNicotiana tabacumt0
- Organ / stage
- leaf and calyxe0
- Gene
- CAT1e0
- Outcome
- CAT1e0
- Direction
- highly expressede0
- Comparator
- roots and seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CAT1 is highly expressed in leaf and calyx, while less expressed in roots and seeds”
t0“Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L”
Claim · model-written summary
Mechanical damage, osmotic pressure, temperature extremes, drought and TMV infection enhanced CAT1 expression.
- Taxon
- Nicotiana tabacum LNicotiana tabacumt0
- Treatment
- mechanical damage, osmotic pressure, low temperature, high temperature, drought, and TMV infectione0
- Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- NC89 CAT1e0
- Outcome
- expression of NC89 CAT1e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of NC89 CAT1 was enhanced by abiotic and biotic stressors, such as mechanical damage, osmotic pressure, low temperature, high temperature, drought, and TMV infection”
t0“Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L”
Claim · model-written summary
Ultraviolet irradiation inhibited CAT1 expression.
- Taxon
- Nicotiana tabacum LNicotiana tabacumt0
- Treatment
- ultraviolet irradiatione0
- Gene
- CAT1t0
- Outcome
- ite0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was inhibited by ultraviolet irradiation”
t0“Cloning of catalase gene(CAT1) and its expression patterns in Nicotiana tabacum L”
The same questions in other papers
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See all 6 papers →mechanical · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
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See all 6 papers →infection · Tobacco mosaic virus · Nicotiana tabacum (tobacco)Positive and negative results
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See all 62 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.