Abstract
Cell viability and DNA fragmentation analysis are essential markers for tomato fruit programmed cell death(PCD).The ion leakage detection and TUNEL assay of tomato fruit method were optimized in this study,and compared the effect of different concentrations of mannitol and water as a medium on determination of ion leakage.The ion leakage of tomato fruit discs was lower in 0.2 mol/L(0.3mol/L of mannitol solution,and became stable after 120 min.But the ion leakage in water increased continually within 240 min,from(23.95± 0.85)% to(63.95±0.35)%.The results showed that immersion of pericarp discs in 0.3mol/L mannitol,120 min shaking was suitable for detecting the ion leakage of tomato fruit.Cell death rate rised in red ripe tomatoes and 45 ℃ heat-treated mature green tomatoes.In situ TUNEL staining using hand slice for detecting DNA fragmentation of tomato fruit cells was simple and feasible,and TUNEL-positive nucleus were detected in senescence tomato fruit and heat-treated mature green tomato fruit,respectively.
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Claims 8
Claim · model-written summary
Tomato disc ion leakage was lower in the reported mannitol solutions and stabilized after 120 minutes.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- 0.2 mol/L(0.3mol/L of mannitol solutione0
- Outcome
- ion leakagee0
- Direction
- lowere0
- Qualifier
- became stable after 120 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ion leakage of tomato fruit discs was lower in 0.2 mol/L(0.3mol/L of mannitol solution,and became stable after 120 min.”
Claim · model-written summary
Ion leakage in water increased from 23.95±0.85% to 63.95±0.35% within 240 minutes.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- watere0
- Outcome
- ion leakagee0
- Direction
- increasede0
- Value
- (63.95±0.35)%e0
- Qualifier
- within 240 min,from(23.95± 0.85)%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“But the ion leakage in water increased continually within 240 min,from(23.95± 0.85)% to(63.95±0.35)%.”
t0“Tomato”
Claim · model-written summary
Immersion in 0.3mol/L mannitol with 120 minutes shaking was suitable for tomato ion leakage detection.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- immersion of pericarp discs in 0.3mol/L mannitol,120 min shakinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed that immersion of pericarp discs in 0.3mol/L mannitol,120 min shaking was suitable for detecting the ion leakage of tomato fruit.”
Claim · model-written summary
Cell death rate rose in red ripe tomatoes.
- Taxon
- tomatoesSolanum lycopersicume0
- Organ / stage
- red ripee0
- Outcome
- Cell death ratee0
- Direction
- risede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell death rate rised in red ripe tomatoes and 45 ℃ heat-treated mature green tomatoes.”
Claim · model-written summary
Heat treatment at 45 ℃ increased cell death rate in mature green tomatoes.
- Taxon
- tomatoesSolanum lycopersicume0
- Organ / stage
- mature greene0
- Treatment
- 45 ℃ heat-treatede0
- Outcome
- Cell death ratee0
- Direction
- risede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cell death rate rised in red ripe tomatoes and 45 ℃ heat-treated mature green tomatoes.”
Claim · model-written summary
Hand-slice TUNEL staining was simple and feasible for detecting tomato DNA fragmentation.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- In situ TUNEL staining using hand slicee0
- Outcome
- simple and feasiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In situ TUNEL staining using hand slice for detecting DNA fragmentation of tomato fruit cells was simple and feasible”
Claim · model-written summary
TUNEL-positive nuclei were detected in senescent tomato fruit.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- senescence tomato fruite0
- Outcome
- TUNEL-positive nucleus were detectede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TUNEL-positive nucleus were detected in senescence tomato fruit and heat-treated mature green tomato fruit,respectively.”
Claim · model-written summary
TUNEL-positive nuclei were detected in heat-treated mature green tomato fruit.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- mature green tomato fruite0
- Treatment
- heat-treatede0
- Outcome
- TUNEL-positive nucleus were detectede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TUNEL-positive nucleus were detected in senescence tomato fruit and heat-treated mature green tomato fruit,respectively.”
The same questions in other papers
heat · cytology · Solanum lycopersicum (tomato)Results differ
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.