Abstract
Tomato plants overexpressing the disease resistance gene Pto (35S::Pto) exhibit spontaneous cell death, accumulation of salicylic acid (SA), elevated expression of pathogenesis-related genes, and enhanced resistance to a broad range of pathogens. Because salicylate plays an important role in the cell death and defense activation in many lesion mimic mutants, we investigated the interaction of SA-mediated processes and the 35S::Pto-mediated defense pathway by introducing the nahG transgene that encodes salicylate hydroxylase. Here, we show that SA is not required for the 35S::Pto-activated microscopic cell death and plays a minor role in defense gene activation and general disease resistance in 35S::Pto plants. In contrast, temperature greatly affects the spontaneous cell death and general resistance in 35S::Pto plants, and high temperature inhibits the cell death. The NahG tomato plants develop spontaneous, unconstrained necrotic lesions on leaves. These lesions also are initiated by the inoculation of a virulent strain of Pseudomonas syringae pv. tomato. However, the NahG-dependent necrotic lesions are inhibited in the NahG/35S::Pto plants. This inhibition is most pronounced under conditions favoring the 35S::Pto-mediated spontaneous cell death development. These results indicate that the signaling pathways activated by Pto overexpression suppress the cellular damage that is caused by SA depletion. We also found that ethylene is dispensable for the 35S::Pto-mediated general defense.
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Claims 10
Claim · model-written summary
Salicylic acid is not required for 35S::Pto-activated microscopic cell death in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- nahG transgenee2
- Gene
- 35S::Ptoe1
- Outcome
- microscopic cell deathe1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e1“Here, we show that SA is not required for the 35S::Pto-activated microscopic cell death and plays a minor role in defense gene activation and general disease resistance in 35S::Pto plants”
e2“introducing the nahG transgene that encodes salicylate hydroxylase”
Claim · model-written summary
Salicylic acid plays a minor role in defense gene activation and general disease resistance in 35S::Pto tomato plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- nahG transgenee2
- Gene
- 35S::Ptoe1
- Outcome
- defense gene activation and general disease resistancee1
- Qualifier
- minor rolee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e1“Here, we show that SA is not required for the 35S::Pto-activated microscopic cell death and plays a minor role in defense gene activation and general disease resistance in 35S::Pto plants”
e2“introducing the nahG transgene that encodes salicylate hydroxylase”
Claim · model-written summary
High temperature inhibited spontaneous cell death in 35S::Pto tomato plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- high temperaturee3
- Outcome
- spontaneous cell deathe3
- Direction
- inhibitse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e3“temperature greatly affects the spontaneous cell death and general resistance in 35S::Pto plants, and high temperature inhibits the cell death”
Claim · model-written summary
Temperature greatly affects general resistance in 35S::Pto tomato plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- temperaturee3
- Outcome
- general resistancee3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e3“temperature greatly affects the spontaneous cell death and general resistance in 35S::Pto plants, and high temperature inhibits the cell death”
Claim · model-written summary
NahG tomato plants develop spontaneous, unconstrained necrotic lesions on leaves.
- Taxon
- tomatoSolanum lycopersicume4
- Material
- NahGe4
- Organ / stage
- leavese4
- Outcome
- spontaneous, unconstrained necrotic lesionse4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The NahG tomato plants develop spontaneous, unconstrained necrotic lesions on leaves”
Claim · model-written summary
Inoculation with a virulent Pseudomonas syringae pv. tomato strain also initiated necrotic lesions in NahG tomato plants.
- Taxon
- tomatoSolanum lycopersicume4
- Treatment
- inoculation of a virulent strain of Pseudomonas syringae pv. tomatoe5
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe5pathogen - Outcome
- necrotic lesionse4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The NahG tomato plants develop spontaneous, unconstrained necrotic lesions on leaves”
e5“These lesions also are initiated by the inoculation of a virulent strain of Pseudomonas syringae pv. tomato”
Claim · model-written summary
NahG-dependent necrotic lesions were inhibited in NahG/35S::Pto tomato plants compared with NahG plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- 35S::Ptoe6
- Outcome
- NahG-dependent necrotic lesionse6
- Direction
- inhibitede6
- Comparator
- NahGe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e6“However, the NahG-dependent necrotic lesions are inhibited in the NahG/35S::Pto plants”
Claim · model-written summary
Inhibition of necrotic lesions was most pronounced under conditions favouring 35S::Pto-mediated spontaneous cell death.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- conditions favoring the 35S::Pto-mediated spontaneous cell death developmente7
- Outcome
- inhibitione7
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e7“This inhibition is most pronounced under conditions favoring the 35S::Pto-mediated spontaneous cell death development”
Claim · model-written summary
Signalling pathways activated by Pto overexpression suppress the cellular damage caused by salicylate depletion in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Pto overexpressione1
- Outcome
- cellular damage that is caused by SA depletione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e1“These results indicate that the signaling pathways activated by Pto overexpression suppress the cellular damage that is caused by SA depletion”
Claim · model-written summary
Ethylene is dispensable for the 35S::Pto-mediated general defense in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Compound
- ethylenee1
- Outcome
- general defensee1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing the disease resistance gene Pto (35S::Pto)”
e1“We also found that ethylene is dispensable for the 35S::Pto-mediated general defense”
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