Abstract
Strain Bacillus subtilis T2 is a potential biocontrol agent against a broad-spectrum plant pathogenic fungi and can produce extracellular phytase. To evaluate whether the phytase secreted by T2 can enhance the saline tolerance of plant, mutagenetic program was undertaken for the construction of extracellular phytase derivates. A phytase minus mutant M3 with genetic stability was obtained by means of DES treatment. The results from biochemical experiments revealed that the culture filtrates of wild-type strain T2 with phytase activity and purified phytase stimulated growth of wheat seedlings under phosphate limitation in the presence of 150 mmol L-1 NaCl and phytate. Significant increases in plant height, fresh weight, total leaf chlorophyll content and TTC reducing power and decrease in content of MDA were observed in response to the addition of phytase and culture filtrates of T2 to culture liquids. However, the culture filtrates obtained from phytase-negative mutant strain M3 did not stimulate the growth of wheat seedlings under salt stress. These may provide strong evidence that phytase activity of B. subtilis is important for the increasing resistance of wheat to salt stress under phosphate limitation.
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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Culture filtrates of wild-type T2 with phytase activity and purified phytase stimulated growth of wheat seedlings under phosphate limitation with NaCl and phytate.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- culture filtrates of wild-type strain T2 with phytase activity and purified phytasee1
- Interactor
- Bacillus subtilis T2
Bacillus subtilist0symbiont - Outcome
- growthe1
- Direction
- stimulatede1
- Qualifier
- under phosphate limitation in the presence of 150 mmol L-1 NaCl and phytatee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings”
e1“the culture filtrates of wild-type strain T2 with phytase activity and purified phytase stimulated growth of wheat seedlings under phosphate limitation in the presence of 150 mmol L-1 NaCl and phytate”
Claim · model-written summary
Addition of phytase and T2 culture filtrates increased plant height, fresh weight and total leaf chlorophyll content of wheat seedlings under salt stress.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- addition of phytase and culture filtrates of T2e2
- Outcome
- plant height, fresh weight, total leaf chlorophyll contente2
- Direction
- increasese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings”
e2“Significant increases in plant height, fresh weight, total leaf chlorophyll content and TTC reducing power and decrease in content of MDA were observed in response to the addition of phytase and culture filtrates of T2 to culture liquids”
Claim · model-written summary
Addition of phytase and T2 culture filtrates increased TTC reducing power of wheat seedlings.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- addition of phytase and culture filtrates of T2e2
- Outcome
- TTC reducing powere2
- Direction
- increasese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings”
e2“Significant increases in plant height, fresh weight, total leaf chlorophyll content and TTC reducing power and decrease in content of MDA were observed in response to the addition of phytase and culture filtrates of T2 to culture liquids”
Claim · model-written summary
Addition of phytase and T2 culture filtrates decreased MDA content of wheat seedlings.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- addition of phytase and culture filtrates of T2e2
- Outcome
- content of MDAe2
- Direction
- decreasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings”
e2“Significant increases in plant height, fresh weight, total leaf chlorophyll content and TTC reducing power and decrease in content of MDA were observed in response to the addition of phytase and culture filtrates of T2 to culture liquids”
Claim · model-written summary
Culture filtrates of the phytase-negative mutant M3 did not stimulate growth of wheat seedlings under salt stress.
- Taxon
- wheatTriticum aestivume3
- Treatment
- culture filtrates obtained from phytase-negative mutant strain M3e3
- Interactor
- Bacillus subtilis T2Bacillus subtilist0symbiont
- Outcome
- growth of wheat seedlingse3
- Direction
- did not stimulatee3
- Qualifier
- under salt stresse3
- Comparator
- wild-type strain T2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings”
e1“the culture filtrates of wild-type strain T2 with phytase activity and purified phytase stimulated growth of wheat seedlings under phosphate limitation in the presence of 150 mmol L-1 NaCl and phytate”
e3“the culture filtrates obtained from phytase-negative mutant strain M3 did not stimulate the growth of wheat seedlings under salt stress”
Claim · model-written summary
Phytase activity of B. subtilis is suggested to be important for wheat resistance to salt stress under phosphate limitation.
- Taxon
- wheatTriticum aestivume4
- Gene
- phytasee4
- Outcome
- resistance of wheat to salt stresse4
- Qualifier
- under phosphate limitatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings”
e4“These may provide strong evidence that phytase activity of B. subtilis is important for the increasing resistance of wheat to salt stress under phosphate limitation”
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.