Abstract
Using He-Ne laser irradiation to repair the ROP GTPase damage by the enhanced UV-B radiation in wheat seedlings has been studied.We extracted small G protein,and then determined its content by SDS-PAGE electrophoresis.At the same time,we tested the FITC fluorescence intensity of wheat microfilament marker by using a laser scanning confocal microscope.The results showed that,the enhanced UV-B radiation reduced the Rop protein content in wheat seedlings,and we also found that it changed the shape of protoplast by LCSM scanning.The intensity of the fluorescence which marks damaged cytoskeletal by the enhanced UV-B radiation was weaken.Moreover,in the whole period of UV-B treatment,the fluorescence degree was lower than the control group(CK).Then,after treating with the He-Ne laser,the content,the shape and the intensity were all increased,but still lower than the control guoup.From the above descriptions,we can say that the UV-B radiation could decrease the Rop GTPase content of wheat seedlings and damage microfilament cytoskeleton.However,a fixed dose of laser played a role in repairing the low protein content and the cell cytoskeleton damaged by the UV-B radiation.By the way,the Rop proteins participate in the reorganization of the actin cytoskeleton.
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Claims 8
Claim · model-written summary
Enhanced UV-B radiation reduced Rop protein content in wheat seedlings.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- enhanced UV-B radiatione0
- Gene
- Rop proteine0
- Outcome
- Rop protein contente0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enhanced UV-B radiation reduced the Rop protein content in wheat seedlings”
Claim · model-written summary
Enhanced UV-B radiation changed wheat protoplast shape.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- enhanced UV-B radiatione1
- Outcome
- shape of protoplaste0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it changed the shape of protoplast by LCSM scanning”
t0“Wheats”
e1“the enhanced UV-B radiation reduced the Rop protein content in wheat seedlings”
Claim · model-written summary
UV-B radiation weakened cytoskeletal marker fluorescence.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- enhanced UV-B radiatione0
- Outcome
- intensity of the fluorescencee0
- Direction
- weakene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The intensity of the fluorescence which marks damaged cytoskeletal by the enhanced UV-B radiation was weaken.”
t0“Wheats”
Claim · model-written summary
Fluorescence remained below the control throughout UV-B treatment.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- UV-B treatmente0
- Outcome
- fluorescence degreee0
- Direction
- lowere0
- Comparator
- control group(CK)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the whole period of UV-B treatment,the fluorescence degree was lower than the control group(CK).”
t0“Wheats”
Claim · model-written summary
He-Ne laser treatment increased Rop protein content after UV-B exposure, though it remained below control.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- He-Ne lasere0
- Outcome
- contente0
- Direction
- increasede0
- Qualifier
- still lower than the control guoupe0
- Comparator
- control guoupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after treating with the He-Ne laser,the content,the shape and the intensity were all increased,but still lower than the control guoup.”
t0“Wheats”
Claim · model-written summary
He-Ne laser treatment increased protoplast shape measure after UV-B exposure, though it remained below control.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- He-Ne lasere0
- Outcome
- shapee0
- Direction
- increasede0
- Qualifier
- still lower than the control guoupe0
- Comparator
- control guoupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after treating with the He-Ne laser,the content,the shape and the intensity were all increased,but still lower than the control guoup.”
t0“Wheats”
Claim · model-written summary
He-Ne laser treatment increased fluorescence intensity after UV-B exposure, though it remained below control.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- He-Ne lasere0
- Outcome
- intensitye0
- Direction
- increasede0
- Qualifier
- still lower than the control guoupe0
- Comparator
- control guoupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after treating with the He-Ne laser,the content,the shape and the intensity were all increased,but still lower than the control guoup.”
t0“Wheats”
Claim · model-written summary
The authors infer Rop proteins participate in actin cytoskeleton reorganization.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Rop proteinse0
- Outcome
- reorganization of the actin cytoskeletone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Rop proteins participate in the reorganization of the actin cytoskeleton.”
t0“Wheats”
The same questions in other papers
uv · quality: protein · Triticum aestivum (wheat)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.