Abstract
Partial cDNA fragments of two putative receptor-like protein kinases, RLPK2 and RLPK4, were cloned from soybean leaves with/without senescence-inducing treatment by RT-PCR (Fig.1). Sequence alignments (Blastp) suggested they have significant homology with eukaryotic protein kinase domain. FASTA search revealed similarities both between RLPK2 cDNA fragment and Arabidopsis thaliana receptor-like serine/threonine kinase (RKF1) gene (73% identity), and between RLPK4 cDNA fragment and Oryza sativa clone GRLG4 receptor kinase gene (74.519% identity) (Fig.2). Preliminary structural analysis showed a 407 bp intron between the Vib and IX subdomain of RLPK2 (Fig.1). The expression levels of both putative kinase genes in soybean leaf were up-regulated by senescence-inducing treatment. Exogenous 6-BA pretreatment partially reversed the effect of ST on the mRNA accumulation of RLPK2 gene while no obviously effect on gene expression of RLPK4 was detected (Fig.3). The specific expression patterns of these two genes implied their involvement in the soybean leaf senescence process and perhaps also in the senescence-retarding effect of cytokinins.
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Claims 9
Claim · model-written summary
RLPK2 and RLPK4 cDNA fragments were cloned from soybean leaves.
- Taxon
- soybean
Glycine maxe0 - Gene
- RLPK2 and RLPK4e0
- Outcome
- Partial cDNA fragmentse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Partial cDNA fragments of two putative receptor-like protein kinases, RLPK2 and RLPK4, were cloned from soybean leaves”
Claim · model-written summary
The proteins showed significant homology to a eukaryotic protein kinase domain.
- Taxon
- soybeanGlycine maxt0
- Gene
- eukaryotic protein kinase domaine0
- Outcome
- significant homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence alignments (Blastp) suggested they have significant homology with eukaryotic protein kinase domain.”
t0“Soybean”
Claim · model-written summary
RLPK2 had 73 percent identity to Arabidopsis RKF1.
- Taxon
- soybeanGlycine maxt0
- Gene
- RLPK2e0
- Outcome
- identitye0
- Value
- 73%e0
- Comparator
- Arabidopsis thaliana receptor-like serine/threonine kinase (RKF1) genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RLPK2 cDNA fragment and Arabidopsis thaliana receptor-like serine/threonine kinase (RKF1) gene (73% identity)”
t0“Soybean”
Claim · model-written summary
RLPK4 had 74.519 percent identity to a rice receptor kinase gene.
- Taxon
- soybeanGlycine maxt0
- Gene
- RLPK4e0
- Outcome
- identitye0
- Value
- 74.519%e0
- Comparator
- Oryza sativa clone GRLG4 receptor kinase genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RLPK4 cDNA fragment and Oryza sativa clone GRLG4 receptor kinase gene (74.519% identity)”
t0“Soybean”
Claim · model-written summary
RLPK2 contained a 407 bp intron between subdomains Vib and IX.
- Taxon
- soybeanGlycine maxt0
- Gene
- RLPK2e0
- Outcome
- introne0
- Value
- 407 bpe0
- Qualifier
- between the Vib and IX subdomaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 407 bp intron between the Vib and IX subdomain of RLPK2”
t0“Soybean”
Claim · model-written summary
Senescence-inducing treatment up-regulated both kinase genes in soybean leaf.
- Taxon
- soybeanGlycine maxe0
- Treatment
- senescence-inducing treatmente0
- Gene
- both putative kinase genese0
- Outcome
- expression levelse0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression levels of both putative kinase genes in soybean leaf were up-regulated by senescence-inducing treatment.”
Claim · model-written summary
6-BA pretreatment partially reversed the ST effect on RLPK2 mRNA accumulation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Exogenous 6-BA pretreatmente0
- Gene
- RLPK2e0
- Outcome
- mRNA accumulatione0
- Direction
- reversede0
- Comparator
- STe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous 6-BA pretreatment partially reversed the effect of ST on the mRNA accumulation of RLPK2 gene”
t0“Soybean”
Claim · model-written summary
6-BA pretreatment had no obvious effect on RLPK4 expression.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Exogenous 6-BA pretreatmente0
- Gene
- RLPK4e0
- Outcome
- gene expression of RLPK4e0
- Direction
- no obviously effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous 6-BA pretreatment partially reversed the effect of ST on the mRNA accumulation of RLPK2 gene while no obviously effect on gene expression of RLPK4 was detected”
t0“Soybean”
Claim · model-written summary
The authors infer that both genes may participate in soybean leaf senescence and cytokinin retardation.
- Taxon
- soybeanGlycine maxe0
- Gene
- these two genese0
- Outcome
- soybean leaf senescence processe0
- Qualifier
- perhaps also in the senescence-retarding effect of cytokininse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The specific expression patterns of these two genes implied their involvement in the soybean leaf senescence process and perhaps also in the senescence-retarding effect of cytokinins.”
The same questions in other papers
N-benzyladenine · molecular: expression · Glycine max (soybean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.