Abstract
The leaves of aseptic plant were obtained through leaf culture in vitro and then were selected as explants,in order to establish leave regeneration systems for Pyrus ussuriensis.The results showed that when leaves subcultured for 30 days were inoculated in MS medium containing BA 5.0 mg/L+NAA 0.4 mg/L,adventitious shoots appeared 20—30 days later,with regeneration frequency being 48.6% and bud number being 3.83 per leaf.The highest regeneration frequency of leaves was obtained when leaves were cultured for 20 days in the dark.The regeneration frequency and bud number per leaf were higher when abaxial side was placed downward onto medium than upward.Regeneration frequency of the basal part of leaves was higher than that of the middle part or top part,reaching 68.1%.
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Claims 7
Claim · model-written summary
Leaves on MS medium with BA and NAA formed adventitious shoots after 20–30 days.
- Taxon
- Pyrus ussuriensis
Pyrus ussuriensist0 - Treatment
- MS medium containing BA 5.0 mg/L+NAA 0.4 mg/Le0
- Outcome
- adventitious shoots appearede0
- Value
- 20—30 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when leaves subcultured for 30 days were inoculated in MS medium containing BA 5.0 mg/L+NAA 0.4 mg/L,adventitious shoots appeared 20—30 days later”
t0“Pyrus ussuriensis”
Claim · model-written summary
Leaf regeneration frequency reached 48.6% in the stated medium.
- Taxon
- Pyrus ussuriensisPyrus ussuriensist0
- Outcome
- regeneration frequencye0
- Value
- 48.6%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with regeneration frequency being 48.6% and bud number being 3.83 per leaf”
t0“Pyrus ussuriensis”
Claim · model-written summary
Bud number was 3.83 per leaf.
- Taxon
- Pyrus ussuriensisPyrus ussuriensist0
- Outcome
- bud numbere0
- Value
- 3.83 per leafe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with regeneration frequency being 48.6% and bud number being 3.83 per leaf”
t0“Pyrus ussuriensis”
Claim · model-written summary
Twenty days of dark culture gave the highest leaf regeneration frequency.
- Taxon
- Pyrus ussuriensisPyrus ussuriensist0
- Treatment
- 20 days in the darke0
- Outcome
- regeneration frequency of leavese0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest regeneration frequency of leaves was obtained when leaves were cultured for 20 days in the dark.”
t0“Pyrus ussuriensis”
Claim · model-written summary
Abaxial side down increased regeneration frequency relative to upward placement.
- Taxon
- Pyrus ussuriensisPyrus ussuriensist0
- Treatment
- abaxial side was placed downward onto mediume0
- Outcome
- regeneration frequencye0
- Direction
- highere0
- Comparator
- upwarde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regeneration frequency and bud number per leaf were higher when abaxial side was placed downward onto medium than upward.”
t0“Pyrus ussuriensis”
Claim · model-written summary
Abaxial side down increased bud number per leaf relative to upward placement.
- Taxon
- Pyrus ussuriensisPyrus ussuriensist0
- Treatment
- abaxial side was placed downward onto mediume0
- Outcome
- bud number per leafe0
- Direction
- highere0
- Comparator
- upwarde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regeneration frequency and bud number per leaf were higher when abaxial side was placed downward onto medium than upward.”
t0“Pyrus ussuriensis”
Claim · model-written summary
Basal leaf parts regenerated more frequently than middle or top parts, reaching 68.1%.
- Taxon
- Pyrus ussuriensisPyrus ussuriensist0
- Organ / stage
- basal part of leavese0
- Outcome
- Regeneration frequencye0
- Direction
- highere0
- Value
- 68.1%e0
- Comparator
- middle part or top parte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regeneration frequency of the basal part of leaves was higher than that of the middle part or top part,reaching 68.1%.”
t0“Pyrus ussuriensis”
The same questions in other papers
light · growth · Pyrus ussuriensis (Ussurian pear)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.