Abstract
In order to develop a new resistance identification method of sugarcanes to smut disease,caused by the fungus Ustiliago scitaminea Sydow,and provide technical support to breeding of smut resistant sugarcanes,single teliospore of Ustiliago scitaminea Sydow was isolated and cultured,mating type of sporidia was also identified,by using a 1 ∶ 1 mixture(plus and minus) mating types as inoculum. Injection inoculation was carried out at the growing point or young stem below it in younger sugarcane seedlings,and the concentration of mating type(plus and minus) and injection volume were optimized in this study. The results showed that comparatively the most suitable mating type concentration was 5 ×105sporidia/mL,the relatively appropriate injection volume was 90 μL per plant in injection inoculation method. In comparison with common dipping inoculation,injection inoculation has a shorter latent period,high incidence,and it is possible to sufficiently evaluate resistance level of host and the type of physiological race. On this basis,twenty-two sugarcane varieties(materials) were inoculated by using the newly established injection inoculation and common dipping inoculation,the results showed that there were one susceptible variety,twenty-one high susceptible varieties by using injection inoculation method; however,one resistant variety,two moderately resistant varieties,eight susceptible varieties and eleven high susceptible varieties by using common dipping inoculation method.
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Claims 11
Claim · model-written summary
The most suitable mating type concentration for the injection inoculation method was 5 x 10^5 sporidia/mL.
Verbatim
e0“the most suitable mating type concentration was 5 ×105sporidia/mL”
Claim · model-written summary
The relatively appropriate injection volume was 90 microlitres per plant in the injection inoculation method.
Verbatim
e0“the relatively appropriate injection volume was 90 μL per plant in injection inoculation method”
Claim · model-written summary
Injection inoculation of sugarcane with smut had a shorter latent period than common dipping inoculation.
Verbatim
e0“In comparison with common dipping inoculation,injection inoculation has a shorter latent period,high incidence”
t0“sugarcane varieties”
Claim · model-written summary
Injection inoculation of sugarcane with smut gave high incidence compared with common dipping inoculation.
Verbatim
e0“In comparison with common dipping inoculation,injection inoculation has a shorter latent period,high incidence”
t0“sugarcane varieties”
Claim · model-written summary
Injection inoculation makes it possible to sufficiently evaluate the resistance level of the host and the type of physiological race, compared with common dipping inoculation.
Verbatim
e0“In comparison with common dipping inoculation,injection inoculation has a shorter latent period,high incidence,and it is possible to sufficiently evaluate resistance level of host and the type of physiological race”
Claim · model-written summary
With injection inoculation, one of the twenty-two sugarcane varieties was susceptible.
Verbatim
e0“one susceptible variety,twenty-one high susceptible varieties by using injection inoculation method”
t0“sugarcane varieties”
Claim · model-written summary
With injection inoculation, twenty-one of the twenty-two sugarcane varieties were highly susceptible.
Verbatim
e0“one susceptible variety,twenty-one high susceptible varieties by using injection inoculation method”
t0“sugarcane varieties”
Claim · model-written summary
With common dipping inoculation, one sugarcane variety was resistant.
Verbatim
e0“one resistant variety,two moderately resistant varieties,eight susceptible varieties and eleven high susceptible varieties by using common dipping inoculation method”
t0“sugarcane varieties”
Claim · model-written summary
With common dipping inoculation, two sugarcane varieties were moderately resistant.
Verbatim
e0“one resistant variety,two moderately resistant varieties,eight susceptible varieties and eleven high susceptible varieties by using common dipping inoculation method”
t0“sugarcane varieties”
Claim · model-written summary
With common dipping inoculation, eight sugarcane varieties were susceptible.
Verbatim
e0“one resistant variety,two moderately resistant varieties,eight susceptible varieties and eleven high susceptible varieties by using common dipping inoculation method”
t0“sugarcane varieties”
Claim · model-written summary
With common dipping inoculation, eleven sugarcane varieties were highly susceptible.
Verbatim
e0“one resistant variety,two moderately resistant varieties,eight susceptible varieties and eleven high susceptible varieties by using common dipping inoculation method”
t0“sugarcane varieties”
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