Abstract
The cell wall degrading enzymes(CWDEs) such as polygalacturonase(PG),cellulase(Cx),pectin methylgalacturonase(PMG),polygalacturonic acid trans-eliminase(PGTE) and pectin methyl trans-eliminase(PMTE) were produced in improved Marcus′s medium by Rhizoctonia solani Kuhn causing rice sheath blight.Among five CWDEs,PG,Cx and PMG were significantly higher in the activity than PGTE and PMTE.The culture conditions for the CWDE production of the pathogen were studied.It was appropriate to the CWDE production for the pathogen to be cultured in improved(Marcus′s) medium(pH5) at 28 ℃ without shaking for six days.In addition,the pathogen could produce the CWGEs mentioned above in the lesion after inoculated to rice sheath.There were obvious differences in CWDE activity among three parts of the lesion such as outer chlorotic part,brown part and central dead part.The CWDE activity in the outer chlorotic part was the highest,which was about 3.4 times as much as that of control(healthy part).The next was the CWDE activity in the brown part,which was about 2.9 times as much as that of control.The CWDE activity in the central dead part was the lowest,but which was significantly higher than that of control.These results suggested that the CWDEs produced by the pathogen play an important role in lesion formation and expansion.The pectinase(PG,PMG) and cellulase(Cx) could damage the cell membrane of rice sheath and macerate the sheath tissue through single enzyme or mixed enzyme treatment.
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Claims 10
Claim · model-written summary
Rhizoctonia solani produced five cell-wall degrading enzymes in medium.
- Taxon
- rice
Oryza sativae0 - Interactor
- Rhizoctonia solani Kuhn
Rhizoctonia solanie0pathogen - Outcome
- cellulase(Cx),pectin methylgalacturonase(PMG),polygalacturonic acid trans-eliminase(PGTE) and pectin methyl trans-eliminase(PMTE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polygalacturonase(PG),cellulase(Cx),pectin methylgalacturonase(PMG),polygalacturonic acid trans-eliminase(PGTE) and pectin methyl trans-eliminase(PMTE) were produced in improved Marcus′s medium by Rhizoctonia solani Kuhn causing rice sheath blight”
Claim · model-written summary
PG, Cx and PMG activity exceeded PGTE and PMTE.
- Taxon
- riceOryza sativat0
- Gene
- PG,Cx and PMGe0
- Outcome
- activitye0
- Direction
- highere0
- Comparator
- PGTE and PMTEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PG,Cx and PMG were significantly higher in the activity than PGTE and PMTE”
t0“Rice Plants”
Claim · model-written summary
The indicated culture conditions were appropriate for pathogen enzyme production.
- Taxon
- riceOryza sativat0
- Outcome
- CWDE productione0
- Qualifier
- improved(Marcus′s) medium(pH5) at 28 ℃ without shaking for six dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was appropriate to the CWDE production for the pathogen to be cultured in improved(Marcus′s) medium(pH5) at 28 ℃ without shaking for six days”
t0“Rice Plants”
Claim · model-written summary
The pathogen produced enzymes in inoculated rice sheath lesions.
- Taxon
- riceOryza sativae0
- Organ / stage
- rice sheathe0
- Outcome
- CWGEs mentioned above in the lesione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pathogen could produce the CWGEs mentioned above in the lesion after inoculated to rice sheath”
Claim · model-written summary
Enzyme activity was highest in outer chlorotic lesions at about 3.4 times healthy tissue.
- Taxon
- riceOryza sativat0
- Organ / stage
- outer chlorotic parte0
- Outcome
- CWDE activitye0
- Direction
- higheste0
- Value
- 3.4 timese0
- Comparator
- control(healthy part)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CWDE activity in the outer chlorotic part was the highest,which was about 3.4 times as much as that of control(healthy part)”
t0“Rice Plants”
Claim · model-written summary
Enzyme activity in brown lesion tissue was about 2.9 times healthy tissue.
- Taxon
- riceOryza sativat0
- Organ / stage
- brown parte0
- Outcome
- CWDE activitye0
- Direction
- The nexte0
- Value
- 2.9 timese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The next was the CWDE activity in the brown part,which was about 2.9 times as much as that of control”
t0“Rice Plants”
Claim · model-written summary
Enzyme activity in central dead tissue was lowest among lesion regions but above control.
- Taxon
- riceOryza sativat0
- Organ / stage
- central dead parte0
- Outcome
- CWDE activitye0
- Direction
- highere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CWDE activity in the central dead part was the lowest,but which was significantly higher than that of control”
t0“Rice Plants”
Claim · model-written summary
The authors proposed pathogen enzymes contribute to lesion formation and expansion.
- Taxon
- riceOryza sativat0
- Outcome
- lesion formation and expansione0
- Qualifier
- CWDEs produced by the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CWDEs produced by the pathogen play an important role in lesion formation and expansion”
t0“Rice Plants”
Claim · model-written summary
Pectinase damaged sheath cell membranes and macerated tissue.
- Taxon
- riceOryza sativae0
- Treatment
- pectinase(PG,PMG)e0
- Outcome
- damage the cell membrane of rice sheath and macerate the sheath tissuee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pectinase(PG,PMG) and cellulase(Cx) could damage the cell membrane of rice sheath and macerate the sheath tissue through single enzyme or mixed enzyme treatment”
Claim · model-written summary
Cellulase damaged sheath cell membranes and macerated tissue.
- Taxon
- riceOryza sativae0
- Treatment
- cellulase(Cx)e0
- Outcome
- damage the cell membrane of rice sheath and macerate the sheath tissuee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pectinase(PG,PMG) and cellulase(Cx) could damage the cell membrane of rice sheath and macerate the sheath tissue through single enzyme or mixed enzyme treatment”
The same questions in other papers
pectin · pest · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.