Abstract
In this research, two EREBP (ethylene response element binding protein) genes were isolated by the yeast one-hybrid system, named GhEREB2 and GhEREB3, and both have one intron in their coding regions. The deduced amino acid sequences of GhEREB2 and GhEREB3 have some typical features of transcription factors, one potential basic nuclear-localization signal, one possible acidic activation domain, and one conserved DNA binding domain, and they show high similarity, especially in the DNA-binding domain. GhEREB2 was expressed in roots, stems, and leaves (low levels), but GhEREB3 was only expressed in leaves. Furthermore, the expression of GhEREB2 and GhEREB3 was induced by ethylene and jasmonic acid. In addition, GhEREB2 and GhEREB3 proteins specifically bind to a GCC-box and strongly activate the expression of HIS3 and LacZ reporter genes in yeast. Collectively, these results suggest that GhEREB2 and GhEREB3 might be involved as positive transcription factors in biotic stress signal transduction pathways.
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Claims 7
Claim · model-written summary
GhEREB2 and GhEREB3 were isolated and each had one coding-region intron.
- Taxon
- cotton
Gossypium hirsutumt0 - Gene
- GhEREB2 and GhEREB3e0
- Outcome
- one intron in their coding regionse0
- Value
- one introne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two EREBP (ethylene response element binding protein) genes were isolated by the yeast one-hybrid system, named GhEREB2 and GhEREB3, and both have one intron in their coding regions.”
t0“cotton”
Claim · model-written summary
GhEREB2 and GhEREB3 proteins had typical transcription-factor domains and high sequence similarity.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhEREB2 and GhEREB3e0
- Outcome
- high similaritye0
- Qualifier
- especially in the DNA-binding domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequences of GhEREB2 and GhEREB3 have some typical features of transcription factors, one potential basic nuclear-localization signal, one possible acidic activation domain, and one conserved DNA binding domain, and they show high similarity, especially in the DNA-binding domain.”
t0“cotton”
Claim · model-written summary
GhEREB2 was expressed in roots, stems, and at low levels in leaves.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- roots, stems, and leavese0
- Gene
- GhEREB2e0
- Outcome
- expressede0
- Qualifier
- low levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhEREB2 was expressed in roots, stems, and leaves (low levels)”
t0“cotton”
Claim · model-written summary
GhEREB3 was expressed only in leaves.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhEREB3 was only expressed in leaves.”
t0“cotton”
Claim · model-written summary
Ethylene and jasmonic acid induced expression of both GhEREB genes.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- ethylene and jasmonic acide0
- Outcome
- expression of GhEREB2 and GhEREB3e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of GhEREB2 and GhEREB3 was induced by ethylene and jasmonic acid.”
t0“cotton”
Claim · model-written summary
GhEREB2 and GhEREB3 proteins bound a GCC-box and activated HIS3 and LacZ reporters in yeast.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhEREB2 and GhEREB3 proteinse0
- Outcome
- bind to a GCC-box and strongly activate the expression of HIS3 and LacZ reporter genese0
- Setting
- yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhEREB2 and GhEREB3 proteins specifically bind to a GCC-box and strongly activate the expression of HIS3 and LacZ reporter genes in yeast.”
t0“cotton”
Claim · model-written summary
The authors suggested GhEREB2 and GhEREB3 may act as positive transcription factors in biotic stress signaling.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhEREB2 and GhEREB3e0
- Outcome
- biotic stress signal transduction pathwayse0
- Qualifier
- might be involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhEREB2 and GhEREB3 might be involved as positive transcription factors in biotic stress signal transduction pathways.”
t0“cotton”
The same questions in other papers
ethene · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
6 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
5 papers more in this group
See all 9 papers →jasmonic acid · molecular: expression · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.