Abstract
Using immature cotyledon of four main soybean cultivars of Dongbei district, this paper focused on the influence of medium component of nitrogen? auxin and sucrose, pH and low temperature pretreatment on somatic embryo induction; For the first time, low temperature pretreatment and pH=7.0 medium were applied to somatic embryo induction. The results showed that, High efficient medium of inducing somatic embryo was MSB+40 mg/L 2,4-D +6% sucrose which attained the highest percentage of somatic embryo induction and the efficiency of induction, 49% and 2.94% respectively in this work. Immature cotyledon pretreated with 4℃ and induced on pH=7.0 medium achieved 4.46% efficiency of induction, which is 1% significant difference with the result from control. The interaction between genotypes and duration of low temperature pretreatment exits and the optimal low temperature pretreatment duration of different genotypes varied from 1 to 3 days.
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Claims 4
Claim · model-written summary
MSB medium with 2,4-D and sucrose gave 49% somatic embryo induction.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- MSB+40 mg/L 2,4-D +6% sucrosee0
- Outcome
- percentage of somatic embryo inductione0
- Direction
- higheste0
- Value
- 49%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High efficient medium of inducing somatic embryo was MSB+40 mg/L 2,4-D +6% sucrose which attained the highest percentage of somatic embryo induction and the efficiency of induction, 49% and 2.94% respectively in this work.”
t0“Soybean”
Claim · model-written summary
The same medium gave 2.94% induction efficiency.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MSB+40 mg/L 2,4-D +6% sucrosee0
- Outcome
- efficiency of inductione0
- Direction
- higheste0
- Value
- 2.94%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High efficient medium of inducing somatic embryo was MSB+40 mg/L 2,4-D +6% sucrose which attained the highest percentage of somatic embryo induction and the efficiency of induction, 49% and 2.94% respectively in this work.”
t0“Soybean”
Claim · model-written summary
Cold pretreatment followed by pH 7 medium achieved 4.46% induction efficiency, significantly different from control.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- pretreated with 4℃ and induced on pH=7.0 mediume0
- Outcome
- efficiency of inductione0
- Value
- 4.46%e0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immature cotyledon pretreated with 4℃ and induced on pH=7.0 medium achieved 4.46% efficiency of induction, which is 1% significant difference with the result from control.”
t0“Soybean”
Claim · model-written summary
Optimal cold pretreatment duration varied by genotype from one to three days.
- Taxon
- SoybeanGlycine maxt0
- Material
- different genotypese0
- Outcome
- optimal low temperature pretreatment duratione0
- Value
- 1 to 3 dayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The interaction between genotypes and duration of low temperature pretreatment exits and the optimal low temperature pretreatment duration of different genotypes varied from 1 to 3 days.”
t0“Soybean”
The same questions in other papers
2,4-D · growth · Glycine max (soybean)Results differ
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- negative
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See all 35 papers →sucrose · growth · Glycine max (soybean)Results agree
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.