Abstract
Cytochrome P450, CYP93A1, is involved in the synthesis of the phytoalexin glyceollin in soybean (Glycine max L. Merr). The gene encoding CYP93A1 has been used as defense marker in soybean cell cultures, however, little is known regarding how this gene is expressed in the intact plant. To further understand the tissue-specific role of CYP93A1 in soybean defense, we analyzed the expression of this gene in mechanically damaged leaves and stems. In leaves, CYP93A1 was constitutively expressed; its expression did not change in response to mechanical damage. In stems, however, expression of CYP93A1 was induced as quickly as 4 h after mechanical damage and remained upregulated for at least 48 h. The induction of CYP93A1 was associated with the synthesis of glyceollins. In comparison to several other defense-related genes encoding cysteine protease inhibitors L1 and R1 and storage proteins vspA and vspB, CYP93A1 was the most strongly induced by stem wounding. The induction of CYP93A1 was observed only locally, not systemically. Similar stem expression patterns were consistently observed among three different soybean genotypes. The strong induction of CYP93A1 in mechanically damaged stems suggests an important role in the soybean stem defense response; therefore, this study expands the use of CYP93A1 as a defense response marker to stems, not just soybean cell cultures.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Soybean leaf CYP93A1 expression was constitutive and unchanged by mechanical damage.
- Taxon
- soybean
Glycine maxe1 - Organ / stage
- leavese0
- Treatment
- mechanical damagee0
- Gene
- CYP93A1e0
- Outcome
- expressione0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In leaves, CYP93A1 was constitutively expressed; its expression did not change in response to mechanical damage”
e1“soybean (Glycine max L. Merr)”
Claim · model-written summary
Stem CYP93A1 expression was induced by four hours after damage and remained elevated for at least 48 hours.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- stemse0
- Treatment
- mechanical damagee0
- Gene
- CYP93A1e0
- Outcome
- expression of CYP93A1e0
- Direction
- inducede0
- Qualifier
- as quickly as 4 h after mechanical damage and remained upregulated for at least 48 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In stems, however, expression of CYP93A1 was induced as quickly as 4 h after mechanical damage and remained upregulated for at least 48 h”
e1“soybean (Glycine max L. Merr)”
Claim · model-written summary
CYP93A1 induction was associated with glyceollin synthesis.
- Taxon
- soybeanGlycine maxe1
- Gene
- CYP93A1e0
- Outcome
- synthesis of glyceollinse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of CYP93A1 was associated with the synthesis of glyceollins”
e1“soybean (Glycine max L. Merr)”
Claim · model-written summary
Stem wounding induced CYP93A1 more strongly than the compared defense genes.
- Taxon
- soybeanGlycine maxe1
- Treatment
- stem woundinge0
- Gene
- CYP93A1e0
- Outcome
- CYP93A1e0
- Direction
- most strongly inducede0
- Comparator
- cysteine protease inhibitors L1 and R1 and storage proteins vspA and vspBe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In comparison to several other defense-related genes encoding cysteine protease inhibitors L1 and R1 and storage proteins vspA and vspB, CYP93A1 was the most strongly induced by stem wounding”
e1“soybean (Glycine max L. Merr)”
Claim · model-written summary
CYP93A1 induction after wounding was local rather than systemic.
- Taxon
- soybeanGlycine maxe1
- Gene
- CYP93A1e0
- Outcome
- observed only locally, not systemicallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of CYP93A1 was observed only locally, not systemically”
e1“soybean (Glycine max L. Merr)”
Claim · model-written summary
Three soybean genotypes showed consistent stem expression patterns.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- steme0
- Outcome
- Similar stem expression patternse0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar stem expression patterns were consistently observed among three different soybean genotypes”
The same questions in other papers
mechanical · molecular: expression · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.