Paper · 1980 · Scientia Sinica

Cyanide-insensitive respiration in corn mitochondria. 1. Comparison between sensitivities of alpha -ketoglutarate and succinate oxidations to cyanide.

Fan Yang; Quan Xing; S.-Y. Wang

8
claims
8
verbatim quotes
1
citations
1
research areas
—
Not open access

Abstract

One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris, while the oxygen uptake of α-ketoglutarate was not affectedat all. In the latter case, a very strong inhibition could occur when both KCN and KSCNwere simultaneously present. After cyanide inhibition of succinate oxidation, the oxygen uptake could subsequentlyreappear by addition of α-ketoglutarate, but at that time the cytochromes c and a stillremained in their reduced state. In contrast to the cyanide-resistance,α-ketoglutarate oxidation was inhibited by antimycin A. It has been suggested that cytochromes a and c are not involved in the cyanide-insensi-tive alternative pathway in corn mitochondria and the branching point is located on the mainrespiratory chain after cytochrome b instead of before it.

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Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

KCN fully inhibited cytochrome oxidase activity in corn mitochondria.

Treatment
One mM KCNe0
Outcome
activity of cytochrome oxidasee0
Direction
fully inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris”

✓ located verbatim in the source · abstract, characters 0–109 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

KCN fully inhibited succinate oxidation in corn mitochondria.

Treatment
One mM KCNe1
Outcome
succinateoxidatione1
Direction
fully inhibitede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris”

✓ located verbatim in the source · abstract, characters 0–109 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

KCN did not affect oxygen uptake with α-ketoglutarate.

Treatment
KCNe2
Outcome
oxygen uptake of α-ketoglutaratee2
Direction
not affectedat alle2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“One mM KCN fully inhibited the activity of cytochrome oxidase and the succinateoxidation in corn mitochondris, while the oxygen uptake of α-ketoglutarate was not affectedat all”

✓ located verbatim in the source · abstract, characters 0–176 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

KCN and KSCN together strongly inhibited oxygen uptake with α-ketoglutarate.

Treatment
KCN and KSCNweree3
Outcome
inhibitione3
Direction
very strong inhibitione3

Not reportedTaxonComparator

Verbatim

e3

“In the latter case, a very strong inhibition could occur when both KCN and KSCNwere simultaneously present”

✓ located verbatim in the source · abstract, characters 178–284 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

α-ketoglutarate restored oxygen uptake after cyanide inhibition of succinate oxidation.

Treatment
addition of α-ketoglutaratee4
Outcome
oxygen uptakee4
Direction
reappeare4

Not reportedTaxonComparator

Verbatim

e4

“After cyanide inhibition of succinate oxidation, the oxygen uptake could subsequentlyreappear by addition of α-ketoglutarate”

✓ located verbatim in the source · abstract, characters 286–410 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Cytochromes c and a remained reduced when oxygen uptake reappeared.

Outcome
cytochromes c and ae5
Direction
reduced statee5

Not reportedTaxon

Verbatim

e5

“but at that time the cytochromes c and a stillremained in their reduced state”

✓ located verbatim in the source · abstract, characters 412–489 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Antimycin A inhibited α-ketoglutarate oxidation.

Treatment
antimycin Ae6
Outcome
α-ketoglutarate oxidatione6
Direction
inhibitede6

Not reportedTaxonComparator

Verbatim

e6

“α-ketoglutarate oxidation was inhibited by antimycin A”

✓ located verbatim in the source · abstract, characters 529–583 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors suggest cytochromes a and c are not involved in the alternative pathway and branching occurs after cytochrome b.

Outcome
cyanide-insensi-tive alternative pathwaye7
Qualifier
after cytochrome be7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“cytochromes a and c are not involved in the cyanide-insensi-tive alternative pathway in corn mitochondria and the branching point is located on the mainrespiratory chain after cytochrome b instead of before it”

✓ located verbatim in the source · abstract, characters 612–821 · show in text ↑

potassium cyanide · molecular: metabolites · Zea mays (maize)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

potassium cyanide · nutrient: content · Zea mays (maize)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.