Abstract
Proton pumps of tonoplast membranes have been studied extensively in vitro, but data concerning their regulation in vivo are lacking. Effects of either anoxia, or the addition of KCN, 2-deoxy-d-glucose (deoxy-glucose), or bafilomycin-A1 (BAF) on vacuolar pH of maize (Zea mays L.) root hair cells were followed by fluorescence microscopy after loading of 2[prime]7[prime]-bis-(2-carboxyethyl)-5-(and-6) carboxyfluorescein. Root hair cells were able to maintain vacuolar acidity for at least 2 h in the presence of either 10 mM KCN or 50 mM deoxy-glucose or during anoxia. Treatments with either deoxy-glucose or KCN reduced total tissue ATP more than anoxia. ADP accumulated during anoxia and treatment with KCN as detected by in vivo 31P-NMR spectroscopy, but not during deoxy-glucose treatment. With control roots and roots treated with deoxy-glucose, the presence of BAF, a specific inhibitor of the V-type ATPase, caused alkalization of the vacuolar pH. However, either in the presence of KCN or under anoxic conditions, BAF was relatively ineffective in dissipating vacuolar acidity. Therefore, under anoxia or in the presence of KCN, unlike the situation with air or deoxy-glucose, the V-type ATPase apparently is not required for maintenance of vacuolar acidity.
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Claims 6
Claim · model-written summary
Maize root hair cells maintained vacuolar acidity for at least 2 h with KCN, deoxy-glucose or anoxia.
- Organ / stage
- root hair cellse1
- Treatment
- 10 mM KCN or 50 mM deoxy-glucose or during anoxiae1
- Outcome
- maintain vacuolar aciditye1
- Qualifier
- for at least 2 he1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuolar pH of maize (Zea mays L.) root hair cells”
e1“Root hair cells were able to maintain vacuolar acidity for at least 2 h in the presence of either 10 mM KCN or 50 mM deoxy-glucose or during anoxia”
Claim · model-written summary
Deoxy-glucose or KCN reduced total tissue ATP more than anoxia did in maize root hairs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuolar pH of maize (Zea mays L.) root hair cells”
e2“Treatments with either deoxy-glucose or KCN reduced total tissue ATP more than anoxia”
Claim · model-written summary
ADP accumulated in maize root hairs during anoxia and KCN treatment, as detected by in vivo 31P-NMR spectroscopy, but not during deoxy-glucose treatment.
- Treatment
- anoxia and treatment with KCNe3
- Outcome
- ADPe3
- Direction
- accumulatede3
- Qualifier
- as detected by in vivo 31P-NMR spectroscopye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuolar pH of maize (Zea mays L.) root hair cells”
e3“ADP accumulated during anoxia and treatment with KCN as detected by in vivo 31P-NMR spectroscopy, but not during deoxy-glucose treatment”
Claim · model-written summary
Bafilomycin A1 caused alkalization of the vacuolar pH of maize root hairs in control roots and deoxy-glucose-treated roots.
- Treatment
- BAFe4
- Gene
- V-type ATPasee4
- Outcome
- vacuolar pHe4
- Direction
- alkalizatione4
- Comparator
- control rootse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuolar pH of maize (Zea mays L.) root hair cells”
e4“With control roots and roots treated with deoxy-glucose, the presence of BAF, a specific inhibitor of the V-type ATPase, caused alkalization of the vacuolar pH”
Claim · model-written summary
Bafilomycin A1 was relatively ineffective in dissipating vacuolar acidity in maize root hairs in the presence of KCN or under anoxia.
- Treatment
- BAFe5
- Outcome
- dissipating vacuolar aciditye5
- Direction
- decreaseinferrede5
- Qualifier
- in the presence of KCN or under anoxic conditionse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuolar pH of maize (Zea mays L.) root hair cells”
e5“However, either in the presence of KCN or under anoxic conditions, BAF was relatively ineffective in dissipating vacuolar acidity”
Claim · model-written summary
The V-type ATPase is apparently not required for maintenance of vacuolar acidity under anoxia or in the presence of KCN in maize root hairs.
- Gene
- V-type ATPasee6
- Outcome
- maintenance of vacuolar aciditye6
- Qualifier
- under anoxia or in the presence of KCNe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“vacuolar pH of maize (Zea mays L.) root hair cells”
e6“the V-type ATPase apparently is not required for maintenance of vacuolar acidity”
e7“Therefore, under anoxia or in the presence of KCN, unlike the situation with air or deoxy-glucose, the V-type ATPase apparently is not required”
The same questions in other papers
potassium cyanide · molecular: metabolites · Zea mays (maize)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.