Abstract
Xanthomonas oryzae pv. oryzae (Xoo) produces a putative effector, XoAvrBs2. We expressed XoAvrBs2 homologously in Xoo with a TAP-tag at the C-terminus to enable quantitative analysis of protein expression and secretion. Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels, and also stimulated a remarkable amount of XoAvrBs2 secretion into the medium. In a T3SS-defective Xoo mutant strain, secretion of the TAPtagged XoAvrBs2 was blocked. Thus, we elucidated the transcriptional and translational expressions of the XoAvrBs2 gene in Xoo was induced in vitro by the interaction with rice and the induced secretion of XoAvrBs2 was T3SSdependent. It is the first report to measure the homologous expression and secretion of XoAvrBs2 in vitro by rice leaf extract. Our system for the quantitative analysis of effector protein expression and secretion could be generally used for the study of host-pathogen interactions.
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Claims 4
Claim · model-written summary
Rice leaf extracts from sensitive and resistant cultivars induced XoAvrBs2 transcription in Xoo.
- Taxon
- rice
Oryza sativae0 - Treatment
- rice leaf extractse0
- Interactor
- Xooe0pathogen
- Gene
- XoAvrBs2e0
- Outcome
- expression of the XoAvrBs2 gene at the transcriptionale0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels”
Claim · model-written summary
Rice leaf extracts from sensitive and resistant cultivars induced XoAvrBs2 translation in Xoo.
- Taxon
- riceOryza sativae0
- Treatment
- rice leaf extractse0
- Interactor
- Xooe0pathogen
- Gene
- XoAvrBs2e0
- Outcome
- translational levelse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels”
Claim · model-written summary
Rice leaf extracts stimulated XoAvrBs2 secretion into the medium.
- Taxon
- riceOryza sativat0
- Treatment
- Rice Leaf Extractt0
- Interactor
- Xoot1pathogen
- Outcome
- XoAvrBs2 secretione0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and also stimulated a remarkable amount of XoAvrBs2 secretion into the medium”
t0“rice leaf extract”
t1“Xoo cells”
Claim · model-written summary
A T3SS defective Xoo mutant blocked XoAvrBs2 secretion.
- Taxon
- RiceOryza sativat0
- Treatment
- T3SS-defective Xoo mutant straine0
- Interactor
- Xooe0pathogen
- Outcome
- secretion of the TAPtagged XoAvrBs2e0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In a T3SS-defective Xoo mutant strain, secretion of the TAPtagged XoAvrBs2 was blocked.”
t0“Rice Leaf Extract”
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