Abstract
Isolated rice embryos were used to investigate the regulatory effects of endosperm extracts and pure sugars on the expression of alpha-amylase gene RAmy3D and a sucrose synthase gene homologous to the maize isozyme Ss2. The high-level expression of RAmy3D in the scutella of isolated embryos could be inhibited by a variety of sugars as well as endosperm extracts from germinated rice grains. Glucose, at a concentration of 250 mM, was most effective in repressing RAmy3D mRNA accumulation. Furthermore, this repression was reversible. Interestingly, RAmy3D repression was always accompanied by the induction of sucrose synthase gene expression. These results support a model in which the expression of alpha-amylase and sucrose synthase genes in the rice scutellum are counter-regulated by the influx of sugars from the endosperm.
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Claims 6
Claim · model-written summary
Several sugars inhibited high-level RAmy3D expression in isolated embryo scutella.
- Taxon
- rice
Oryza sativat0 - Organ / stage
- scutellae0
- Treatment
- a variety of sugarse0
- Gene
- RAmy3De0
- Outcome
- expression of RAmy3De0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high-level expression of RAmy3D in the scutella of isolated embryos could be inhibited by a variety of sugars”
t0“rice”
Claim · model-written summary
Germinated rice endosperm extracts inhibited RAmy3D expression.
- Taxon
- riceOryza sativae0
- Treatment
- endosperm extracts from germinated rice grainse0
- Gene
- RAmy3De0
- Outcome
- expression of RAmy3De0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high-level expression of RAmy3D in the scutella of isolated embryos could be inhibited by a variety of sugars as well as endosperm extracts from germinated rice grains.”
Claim · model-written summary
Glucose at 250 mM most effectively repressed RAmy3D mRNA accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Glucose, at a concentration of 250 mMe0
- Gene
- RAmy3De0
- Outcome
- RAmy3D mRNA accumulatione0
- Direction
- repressinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glucose, at a concentration of 250 mM, was most effective in repressing RAmy3D mRNA accumulation.”
t0“rice”
Claim · model-written summary
Glucose repression of RAmy3D was reversible.
- Taxon
- riceOryza sativat0
- Gene
- RAmy3De1
- Outcome
- repression was reversiblee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, this repression was reversible.”
t0“rice”
e1“RAmy3D mRNA accumulation”
Claim · model-written summary
RAmy3D repression was accompanied by sucrose synthase gene induction.
- Taxon
- riceOryza sativat0
- Gene
- RAmy3De0
- Outcome
- induction of sucrose synthase gene expressione0
- Direction
- inductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RAmy3D repression was always accompanied by the induction of sucrose synthase gene expression.”
t0“rice”
Claim · model-written summary
Authors interpret scutellar alpha-amylase and sucrose synthase as counter-regulated by sugar influx from endosperm.
- Taxon
- riceOryza sativae0
- Organ / stage
- scutellume0
- Gene
- alpha-amylase and sucrose synthase genese0
- Outcome
- counter-regulatede0
- Qualifier
- influx of sugars from the endosperme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of alpha-amylase and sucrose synthase genes in the rice scutellum are counter-regulated by the influx of sugars from the endosperm”
The same questions in other papers
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See all 10 papers →stage · molecular: expression · Oryza sativa (rice)Results agree
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- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.