Abstract
The development of gibberellic acid sensitivity, which is measured by the level of α-amylase activity, has been investigated by comparing enzyme levels in freshly detached wheat and barley caryopses cultured on moist filter paper for various times and temperatures with those in caryopses dried over 35% (w / w) glycerol-water solutions or in caryopses air-dried in detached ears at controlled temperatures and by comparing enzyme levels in de-embryonated caryopses with intact ones, both being cultured in aqueous buffer solutions. Similar amounts of enzyme were produced in caryopses held moist or dried, although the duration of the lag period, and the rate of accumulation of α-amylase were markedly influenced by the length and temperature of main treatments. Likewise similar amounts of enzyme were produced in de-embryonated and intact caryopses cultured in an aqueous buffer if the latter were de- embryonated prior to incubation in solutions of gibberellic acid. However, when intact or de-brushed (removal of the apical tip) caryopses were incubated with GA3, less α-amylase was found in these incubates than in those incubates containing de-embryonated caryopses. These observations were found in incubates of both sensitized freshly harvested and normal mature caryopses. It is concluded that drying is not essential for the development of gibberellic acid sensitivity in the wheat and barley caryopses.
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Claims 5
Claim · model-written summary
Similar amounts of alpha-amylase were produced in wheat and barley caryopses held moist or dried.
- Taxon
- Wheat and Barley
.jpg?width=60)
Hordeum vulgare
Triticum aestivumt0 - Organ / stage
- caryopsese0
- Treatment
- driede0
- Outcome
- amounts of enzymee0
- Direction
- Similare0
- Comparator
- held moiste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“Wheat and Barley Caryopses”
e0“Similar amounts of enzyme were produced in caryopses held moist or dried”
Claim · model-written summary
The duration of the lag period and the rate of alpha-amylase accumulation in wheat and barley caryopses were markedly influenced by the length and temperature of the main treatments.
- Taxon
- Wheat and BarleyHordeum vulgareTriticum aestivumt0
- Treatment
- length and temperature of main treatmentse0
- Outcome
- duration of the lag period, and the rate of accumulation of α-amylasee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat and Barley Caryopses”
e0“the duration of the lag period, and the rate of accumulation of α-amylase were markedly influenced by the length and temperature of main treatments”
Claim · model-written summary
Similar amounts of alpha-amylase were produced in de-embryonated and intact caryopses in aqueous buffer when the intact ones were de-embryonated before incubation in gibberellic acid solutions.
- Taxon
- Wheat and BarleyHordeum vulgareTriticum aestivumt0
- Treatment
- de-embryonatede0
- Outcome
- amounts of enzymee0
- Direction
- similare0
- Comparator
- intact caryopsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat and Barley Caryopses”
e0“similar amounts of enzyme were produced in de-embryonated and intact caryopses cultured in an aqueous buffer if the latter were de- embryonated prior to incubation in solutions of gibberellic acid”
Claim · model-written summary
When intact or de-brushed caryopses were incubated with GA3, less alpha-amylase was found than in incubates containing de-embryonated caryopses.
- Taxon
- Wheat and BarleyHordeum vulgareTriticum aestivumt0
- Treatment
- incubated with GA3e0
- Outcome
- α-amylasee0
- Direction
- lesse0
- Comparator
- de-embryonated caryopsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat and Barley Caryopses”
e0“when intact or de-brushed (removal of the apical tip) caryopses were incubated with GA3, less α-amylase was found in these incubates than in those incubates containing de-embryonated caryopses”
Claim · model-written summary
The authors conclude that drying is not essential for the development of gibberellic acid sensitivity in wheat and barley caryopses.
- Taxon
- wheat and barleyHordeum vulgareTriticum aestivume0
- Treatment
- dryinge0
- Outcome
- development of gibberellic acid sensitivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“drying is not essential for the development of gibberellic acid sensitivity in the wheat and barley caryopses”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.