Abstract
Cotyledonary-stage somatic embryos (5-7 mm in length) originating from leaf explants of grape (Vitis vinifera L.) cv. Pusa seedless were encapsulated individually in 2 % alginate gel. The encapsulated somatic embryos (ESEs) germinated successfully on 0.7 % agar medium containing B5 macrosalts (half strength), MURASHIGE and SKOOG microsalts (full strength), 3 % sucrose and 2.9 μM gibberellic acid. The percentage of germination of ESEs was higher than that of nonencapsulated somatic embryos (NSEs) of the same size on the same medium. The percent germination of ESEs increased (69.2 ±2.8) on medium supplemented with quarter strength B5 macrosalts. Of the germinating ESEs, 36 % developed into plantlets. Abscisic acid at 0.004 and 0.02 μM had no significant influence on the frequency of germination and plantlet development, however resulted in a 4-week delay in germination. Transferring the embryos onto the full-strength B5 medium containing sucrose and ABA (0.04 μM) for 4-6 weeks prior to encapsulation resulted in extended storage of up to 90 d without loss of the germination potential and the capacity to regenerate into plantlets. Normally developed plantlets regenerated from ESEs were successfully adapted to soil.
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Claims 8
Claim · model-written summary
Encapsulated somatic embryos germinated on the specified medium.
- Taxon
- grape (Vitis vinifera L.)
Vitis viniferae1 - Material
- Pusa seedlesse1
- Organ / stage
- somatic embryose0
- Treatment
- 0.7 % agar medium containing B5 macrosalts (half strength), MURASHIGE and SKOOG microsalts (full strength), 3 % sucrose and 2.9 μM gibberellic acide0
- Outcome
- germinated successfullye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The encapsulated somatic embryos (ESEs) germinated successfully on 0.7 % agar medium containing B5 macrosalts (half strength), MURASHIGE and SKOOG microsalts (full strength), 3 % sucrose and 2.9 μM gibberellic acid”
e1“leaf explants of grape (Vitis vinifera L.) cv. Pusa seedless”
Claim · model-written summary
Encapsulation increased embryo germination percentage over nonencapsulated embryos of the same size.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Material
- Pusa seedlesse1
- Treatment
- ESEse0
- Outcome
- percentage of germinatione0
- Direction
- highere0
- Comparator
- nonencapsulated somatic embryos (NSEs)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of germination of ESEs was higher than that of nonencapsulated somatic embryos (NSEs) of the same size on the same medium”
e1“leaf explants of grape (Vitis vinifera L.) cv. Pusa seedless”
Claim · model-written summary
Quarter-strength B5 macrosalts increased encapsulated embryo germination to 69.2 ±2.8.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Treatment
- medium supplemented with quarter strength B5 macrosaltse0
- Outcome
- percent germination of ESEse0
- Direction
- increasede0
- Value
- 69.2 ±2.8e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percent germination of ESEs increased (69.2 ±2.8) on medium supplemented with quarter strength B5 macrosalts”
e1“leaf explants of grape (Vitis vinifera L.)”
Claim · model-written summary
Thirty-six percent of germinating encapsulated embryos developed into plantlets.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Outcome
- developed into plantletse0
- Value
- 36 %e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of the germinating ESEs, 36 % developed into plantlets”
e1“leaf explants of grape (Vitis vinifera L.)”
Claim · model-written summary
Abscisic acid at 0.004 and 0.02 μM did not significantly affect germination frequency or plantlet development.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Treatment
- Abscisic acid at 0.004 and 0.02 μMe0
- Outcome
- frequency of germination and plantlet developmente0
- Direction
- no significant influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid at 0.004 and 0.02 μM had no significant influence on the frequency of germination and plantlet development”
e1“leaf explants of grape (Vitis vinifera L.)”
Claim · model-written summary
Abscisic acid delayed germination by four weeks.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Treatment
- Abscisic acid at 0.004 and 0.02 μMe0
- Outcome
- germinatione0
- Direction
- delaye0
- Value
- 4-weeke0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid at 0.004 and 0.02 μM had no significant influence on the frequency of germination and plantlet development, however resulted in a 4-week delay in germination”
e1“leaf explants of grape (Vitis vinifera L.)”
Claim · model-written summary
Pre-encapsulation transfer to full-strength B5 with sucrose and ABA extended storage to 90 days without loss of germination or regeneration capacity.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Treatment
- full-strength B5 medium containing sucrose and ABA (0.04 μM) for 4-6 weeks prior to encapsulatione0
- Outcome
- extended storagee0
- Direction
- extendede0
- Value
- up to 90 de0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transferring the embryos onto the full-strength B5 medium containing sucrose and ABA (0.04 μM) for 4-6 weeks prior to encapsulation resulted in extended storage of up to 90 d without loss of the germination potential and the capacity to regenerate into plantlets”
e1“leaf explants of grape (Vitis vinifera L.)”
Claim · model-written summary
Regenerated plantlets from encapsulated embryos adapted successfully to soil.
- Taxon
- grape (Vitis vinifera L.)Vitis viniferae1
- Organ / stage
- plantletse0
- Outcome
- successfully adapted to soile0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Normally developed plantlets regenerated from ESEs were successfully adapted to soil”
e1“leaf explants of grape (Vitis vinifera L.)”
The same questions in other papers
in vitro · growth · Vitis vinifera (wine grape)Positive and negative results
15 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.