Abstract
Long-term pollen storage can make a research program more efficient by making pollen available on an as-needed basis. The purpose of this research was to develop a practical method for long-term pollen storage of pearl millet (Pennisetum americanum L. Leeke). Pollen with 7 percent or less moisture continued to be viable after 1089 days of storage at −73°C. At −18°C, viability of pollen (6 percent or less moisture) has been maintained for 461 days, while viability of 8 percent or less moisture pollen has been maintained for 816 days at 5°C. In general, pollen with less than 7 percent moisture at storage time was the most favorable for maintaining long-term viability at storage temperatures of −73°C, −18°C, and 5°C. Plastic ziplock bags and glass vials were both effective for storing pollen at −73°C but the pollen in plastic bags gained moisture at −18°C and 5°C, which shortened maintenance of pollen viability. Pollen also was effectively stored in the original glassine collection bags if the pollen was first dried in the bags and the glassine bags were folded and placed in zip-lock plastic bags. Data indicated that storage temperatures of −73°C, −18°C, and 5°C were all effective for maintaining pollen viability if the original desirable pollen moisture content was maintained in air-tight containers such as glass vials.
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Claims 10
Claim · model-written summary
Pollen with 7% or less moisture stored at -73°C remained viable for 1089 days.
- Taxon
- pearl millet
Cenchrus americanust0 - Treatment
- storage at −73°Ce1
- Outcome
- continued to be viablee1
- Value
- 1089 dayse1
- Qualifier
- 7 percent or less moisturee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e1“Pollen with 7 percent or less moisture continued to be viable after 1089 days of storage at −73°C”
Claim · model-written summary
Pollen with 6% or less moisture stored at -18°C remained viable for 461 days.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- at −18°Ce2
- Outcome
- viability of pollene2
- Value
- 461 dayse2
- Qualifier
- 6 percent or less moisturee2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e2“At −18°C, viability of pollen (6 percent or less moisture) has been maintained for 461 days”
Claim · model-written summary
Pollen with 8% or less moisture stored at 5°C remained viable for 816 days.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- at 5°Ce3
- Outcome
- viabilitye3
- Value
- 816 dayse3
- Qualifier
- 8 percent or less moisturee3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e3“while viability of 8 percent or less moisture pollen has been maintained for 816 days at 5°C”
Claim · model-written summary
Pollen with less than 7% moisture at storage time was the most favorable for long-term viability at -73°C, -18°C and 5°C.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- pollen with less than 7 percent moisture at storage timee4
- Outcome
- maintaining long-term viabilitye4
- Direction
- most favorablee4
- Qualifier
- at storage temperatures of −73°C, −18°C, and 5°Ce4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e4“pollen with less than 7 percent moisture at storage time was the most favorable for maintaining long-term viability at storage temperatures of −73°C, −18°C, and 5°C”
Claim · model-written summary
Plastic ziplock bags were effective for storing pollen at -73°C.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- Plastic ziplock bagse5
- Outcome
- storing pollene5
- Direction
- effectivee5
- Qualifier
- at −73°Ce5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e5“Plastic ziplock bags and glass vials were both effective for storing pollen at −73°C”
Claim · model-written summary
Glass vials were effective for storing pollen at -73°C.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- glass vialse5
- Outcome
- storing pollene5
- Direction
- effectivee5
- Qualifier
- at −73°Ce5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e5“Plastic ziplock bags and glass vials were both effective for storing pollen at −73°C”
Claim · model-written summary
Pollen stored in plastic bags at -18°C and 5°C gained moisture.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- plastic bagse6
- Outcome
- moisturee6
- Direction
- gainede6
- Qualifier
- at −18°C and 5°Ce6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e6“the pollen in plastic bags gained moisture at −18°C and 5°C”
Claim · model-written summary
Moisture gain in plastic bags at -18°C and 5°C shortened the maintenance of pollen viability.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- plastic bagse6
- Outcome
- maintenance of pollen viabilitye7
- Direction
- shortenede7
- Qualifier
- at −18°C and 5°Ce6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e6“the pollen in plastic bags gained moisture at −18°C and 5°C”
e7“which shortened maintenance of pollen viability”
Claim · model-written summary
Pollen dried in the original glassine collection bags, with the bags folded and placed in zip-lock plastic bags, was effectively stored.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- original glassine collection bagse7
- Outcome
- effectively storede7
- Qualifier
- if the pollen was first dried in the bags and the glassine bags were folded and placed in zip-lock plastic bagse7
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e7“Pollen also was effectively stored in the original glassine collection bags if the pollen was first dried in the bags and the glassine bags were folded and placed in zip-lock plastic bags”
Claim · model-written summary
Storage at -73°C, -18°C and 5°C was effective for maintaining pollen viability if original moisture was kept in air-tight containers.
- Taxon
- pearl milletCenchrus americanust0
- Treatment
- storage temperatures of −73°C, −18°C, and 5°Ce0
- Outcome
- maintaining pollen viabilitye0
- Direction
- effectivee0
- Qualifier
- if the original desirable pollen moisture content was maintained in air-tight containerse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pearl millet pollen”
e0“storage temperatures of −73°C, −18°C, and 5°C were all effective for maintaining pollen viability if the original desirable pollen moisture content was maintained in air-tight containers”
The same questions in other papers
storage · growth · Cenchrus americanus (pearl millet)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
1 paper more in this group
See all 5 papers →storage · yield · Cenchrus americanus (pearl millet)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.