Abstract
A simple and reliable protocol for micropropagation during 12 subcultures of two field growth male plants of kiwifruit [ Actinidia deliciosa (A.Chev.) Liang and Ferguson] is described. The best results of shoot multiplication and elongation were obtained in Cheng's K(h) medium in the presence of 0.5 μ m NAA, 22 μ m BA and 1.4 μ m GA 3 for `Tomuri' explants, and of 0.1 μ m NAA, 4.4 μ m BA, and 0.3 μ m GA 3 for clone A explants. In addition, the cytokinin compounds TDZ and mT were also tested allowing improving the multiplication rate in `Tomuri' explants. For rooting, `Tomuri' and clone A developed shoots were treated by basal immersion in a 5 m m IBA solution for 15 seconds. Treated shoots were then cultured in half-strength K(h) medium without growth regulators showing 100% rooting after 30 days. Regenerated plantlets were successfully transplanted to soil (90% survival) and they are actively growing in the field. Somaclonal variation analysis by AFLP was carried out using 15 primer combinations, yielding reproducible and well-resolved bands with a 57% of polymorphism. AFLP markers showed to be effective to discriminate genetic variation in this species, being greater in clone A than `Tomuri'. Chemical names used: N 6 -benzyladenine (BA); gibberellic acid (GA 3 ); indole-3-butyric acid (IBA); meta-topolin (mT); naphthaleneacetic acid (NAA); thidiazuron (TDZ).
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Claims 9
Claim · model-written summary
In Cheng's K(h) medium with 0.5 μ m NAA, 22 μ m BA and 1.4 μ m GA 3, Tomuri explants gave the best shoot multiplication and elongation.
- Taxon
- kiwifruit
Actinidia deliciosae0 - Material
- Tomurie1
- Treatment
- Cheng's K(h) medium in the presence of 0.5 μ m NAA, 22 μ m BA and 1.4 μ m GA 3e1
- Outcome
- shoot multiplication and elongatione1
- Direction
- best resultse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e1“The best results of shoot multiplication and elongation were obtained in Cheng's K(h) medium in the presence of 0.5 μ m NAA, 22 μ m BA and 1.4 μ m GA 3 for `Tomuri' explants, and of 0.1 μ m NAA, 4.4 μ m BA, and 0.3 μ m GA 3 for clone A explants”
Claim · model-written summary
In clone A, 0.1 μ m NAA, 4.4 μ m BA and 0.3 μ m GA 3 gave the best shoot multiplication and elongation.
- Taxon
- kiwifruitActinidia deliciosae0
- Material
- clone Ae1
- Treatment
- 0.1 μ m NAA, 4.4 μ m BA, and 0.3 μ m GA 3e1
- Outcome
- shoot multiplication and elongatione1
- Direction
- best resultse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e1“The best results of shoot multiplication and elongation were obtained in Cheng's K(h) medium in the presence of 0.5 μ m NAA, 22 μ m BA and 1.4 μ m GA 3 for `Tomuri' explants, and of 0.1 μ m NAA, 4.4 μ m BA, and 0.3 μ m GA 3 for clone A explants”
Claim · model-written summary
The cytokinins TDZ and mT improved the multiplication rate of Tomuri explants.
- Taxon
- kiwifruitActinidia deliciosae0
- Material
- Tomurie2
- Treatment
- the cytokinin compounds TDZ and mTe2
- Outcome
- multiplication ratee2
- Direction
- improvinge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e2“In addition, the cytokinin compounds TDZ and mT were also tested allowing improving the multiplication rate in `Tomuri' explants”
Claim · model-written summary
Shoots treated by basal immersion in 5 mM IBA rooted at 100% after 30 days on half-strength K(h) medium without growth regulators.
- Taxon
- kiwifruitActinidia deliciosae0
- Material
- `Tomuri' and clone Ae3
- Treatment
- basal immersion in a 5 m m IBA solution for 15 secondse3
- Outcome
- rootinge3
- Value
- 100%e3
- Qualifier
- after 30 dayse3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e3“For rooting, `Tomuri' and clone A developed shoots were treated by basal immersion in a 5 m m IBA solution for 15 seconds. Treated shoots were then cultured in half-strength K(h) medium without growth regulators showing 100% rooting after 30 days”
Claim · model-written summary
Regenerated plantlets transplanted to soil showed 90% survival.
- Taxon
- kiwifruitActinidia deliciosae0
- Outcome
- survivale4
- Value
- 90%e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e4“Regenerated plantlets were successfully transplanted to soil (90% survival)”
Claim · model-written summary
Regenerated kiwifruit plants are actively growing in the field.
- Taxon
- kiwifruitActinidia deliciosae0
- Outcome
- actively growinge5
- Setting
- in the fielde5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e5“they are actively growing in the field”
Claim · model-written summary
AFLP analysis of the regenerants gave 57% polymorphism using 15 primer combinations.
- Taxon
- kiwifruitActinidia deliciosae0
- Treatment
- 15 primer combinationse6
- Outcome
- polymorphisme6
- Value
- 57%e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e6“using 15 primer combinations, yielding reproducible and well-resolved bands with a 57% of polymorphism”
Claim · model-written summary
AFLP markers were effective at discriminating genetic variation in the kiwifruit clones, with more variation in clone A than in Tomuri.
- Taxon
- kiwifruitActinidia deliciosae0
- Material
- clone Ae7
- Outcome
- genetic variatione7
- Direction
- greatere7
- Comparator
- Tomurie7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e7“AFLP markers showed to be effective to discriminate genetic variation in this species, being greater in clone A than `Tomuri'”
Claim · model-written summary
AFLP markers were effective at discriminating genetic variation in this kiwifruit species.
- Taxon
- kiwifruitActinidia deliciosae0
- Outcome
- discriminate genetic variatione7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two field growth male plants of kiwifruit”
e7“AFLP markers showed to be effective to discriminate genetic variation in this species, being greater in clone A than `Tomuri'”
The same questions in other papers
indole-3-butyric acid · growth · Actinidia deliciosa (kiwifruit)Results differ
14 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
12 papers more in this group
See all 16 papers →application · growth · Actinidia deliciosa (kiwifruit)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.