Abstract
Arsenic (As) is classified as one of toxic metalloids, that directly affects to most plants. Moreover, As is also a human carcinogen due to drinking As-contaminated groundwater or eating plant food including rice cultivated in polluted areas. The effect of As on rice growth was established, but the precised mechanism of rice response to As remains to be elucidated. This study investigated the biochemical and molecular changes in roots of rice seedlings under As stress. A 100 mM As(III) solution strongly inhibited the growth and induced reactive oxygen species (ROS) production in rice roots. Using ICP-MS, we found As content in rice roots increased with increasing concentration of As solution treatment. Semi-quantitative RT-PCR analysis revealed that some ROS-related gene, protein kinase-related genes, signal transduction-related genes, transcription factor-related genes and auxin synthesis-related gene were up-regulated during As stress. However, cell cytokinesis-related gene was down-regulated. Polyacrylamide gel electrophoresis and activity-gel assay showed that enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were strongly induced under As stress, as an antioxidant defense system. In addition, the contrileuted to elucidating increase of proline content, function as radical scavenger and cellular redox potential buffer, was found in As treated rice roots. Our findings more or less on the biochemical and molecular mechanism in response to As stress in plants.
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Claims 8
Claim · model-written summary
A 100 mM As(III) solution strongly inhibited rice root growth.
- Taxon
- rice
Oryza sativae1 - Organ / stage
- rice rootse1
- Treatment
- A 100 mM As(III) solutione1
- Outcome
- growthe1
- Direction
- inhibitede1
- Qualifier
- stronglye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“roots of rice seedlings under As stress”
e1“A 100 mM As(III) solution strongly inhibited the growth and induced reactive oxygen species (ROS) production in rice roots”
Claim · model-written summary
A 100 mM As(III) solution induced reactive oxygen species production in rice roots.
- Taxon
- riceOryza sativae1
- Organ / stage
- rice rootse1
- Treatment
- A 100 mM As(III) solutione1
- Outcome
- reactive oxygen species (ROS) productione1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A 100 mM As(III) solution strongly inhibited the growth and induced reactive oxygen species (ROS) production in rice roots”
Claim · model-written summary
Increasing As solution concentration increased As content in rice roots.
- Taxon
- riceOryza sativae2
- Organ / stage
- rice rootse2
- Treatment
- increasing concentration of As solution treatmente2
- Outcome
- As contente2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“we found As content in rice roots increased with increasing concentration of As solution treatment”
Claim · model-written summary
During As stress, ROS-related, protein kinase-related and signal transduction-related genes were up-regulated in rice roots.
- Taxon
- riceOryza sativat0
- Treatment
- As stresse4
- Gene
- ROS-related gene, protein kinase-related genes, signal transduction-related genese3
- Outcome
- ROS-related gene, protein kinase-related genes, signal transduction-related genese3
- Direction
- up-regulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“response to arsenic in rice (Oryza sativa L.)”
e3“some ROS-related gene, protein kinase-related genes, signal transduction-related genes”
e4“were up-regulated during As stress”
Claim · model-written summary
During As stress, transcription factor-related and auxin synthesis-related genes were up-regulated in rice roots.
- Taxon
- riceOryza sativat0
- Treatment
- As stresse4
- Gene
- transcription factor-related genes and auxin synthesis-related genee5
- Outcome
- transcription factor-related genes and auxin synthesis-related genee5
- Direction
- up-regulatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“response to arsenic in rice (Oryza sativa L.)”
e4“were up-regulated during As stress”
e5“transcription factor-related genes and auxin synthesis-related gene”
Claim · model-written summary
Under As stress, the cell cytokinesis-related gene was down-regulated in rice.
- Taxon
- riceOryza sativat0
- Treatment
- As stresse4
- Gene
- cell cytokinesis-related genee6
- Outcome
- cell cytokinesis-related genee6
- Direction
- down-regulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“response to arsenic in rice (Oryza sativa L.)”
e4“were up-regulated during As stress”
e6“cell cytokinesis-related gene was down-regulated”
Claim · model-written summary
Under As stress, superoxide dismutase, catalase and peroxidase enzymatic activity was strongly induced in rice.
- Taxon
- riceOryza sativat0
- Treatment
- As stresse7
- Gene
- superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)e7
- Outcome
- enzymatic activitye7
- Direction
- strongly inducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“response to arsenic in rice (Oryza sativa L.)”
e7“enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were strongly induced under As stress”
Claim · model-written summary
Proline content increased in arsenic-treated rice roots.
- Taxon
- riceOryza sativae1
- Organ / stage
- rice rootse1
- Treatment
- As treatede1
- Outcome
- proline contente1
- Direction
- increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“increase of proline content, function as radical scavenger and cellular redox potential buffer, was found in As treated rice roots”
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