Abstract
The present investigation was conducted for mass multiplication of Jatropha curcas L. through axillary bud culture. For this nodal segment from 3-5 months old nursery grown plants were used as explants for axillary bud culture. The sterilization treatment involving dipping explants in 0.1 per cent HgCl2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants. The treatment MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1) and number of shoots per explant (6.3). Among different treatments for root initiation, half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1) and length of longest root (4.9 cm) when established shoots were treated with it. Such produced plantlets showed nearly cent per cent survival after hardening and acclimatization. It showed that explants surface sterilized with 0.1 per cent HgCl2 solution for 5 minutes inoculated in MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA and half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC were best in shoot establishment and root development respectively for mass multiplication of J. curcas L. through axillary bud culture.
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Claims 11
Claim · model-written summary
Mercuric chloride sterilization minimized contamination and maximized nodal explant establishment.
- Taxon
- Jatropha curcas L.
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Jatropha curcast0 - Treatment
- dipping explants in 0.1 per cent HgCl2 solution for 5 minutese0
- Outcome
- minimum contamination and maximum establishment of nodal explantse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“dipping explants in 0.1 per cent HgCl2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants”
t0“Jatropha curcas L.”
Claim · model-written summary
BAP and IAA supplemented MS medium gave 76.1% culture establishment.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAAe0
- Outcome
- establishmente0
- Direction
- higheste0
- Value
- 76.1%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%)”
t0“Jatropha curcas L.”
Claim · model-written summary
That medium yielded shoot initiation in 3.1 days.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAAe1
- Outcome
- number of days taken for shoot initiatione0
- Direction
- beste1
- Value
- 3.1 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“number of days taken for shoot initiation (3.1 days)”
e1“MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days)”
t0“Jatropha curcas L.”
Claim · model-written summary
That medium produced longest shoots of 6.8 cm.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAAe0
- Outcome
- length of longest shoote0
- Direction
- higheste0
- Value
- 6.8 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm)”
t0“Jatropha curcas L.”
Claim · model-written summary
That medium produced 7.1 leaves on the main shoot.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAAe1
- Outcome
- number of leaves on main shoote0
- Direction
- higheste1
- Value
- 7.1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“number of leaves on main shoot (7.1)”
e1“MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1)”
t0“Jatropha curcas L.”
Claim · model-written summary
That medium produced 6.3 shoots per explant.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAAe1
- Outcome
- number of shoots per explante0
- Direction
- higheste1
- Value
- 6.3e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“number of shoots per explant (6.3)”
e1“MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1) and number of shoots per explant (6.3)”
t0“Jatropha curcas L.”
Claim · model-written summary
Fortified half MS medium yielded maximum rooting percentage of 60.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g ACe0
- Outcome
- rooting percentagee0
- Direction
- maximume0
- Value
- 60e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60)”
t0“Jatropha curcas L.”
Claim · model-written summary
Fortified half MS medium minimized root initiation time to 13.3 days.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g ACe0
- Outcome
- time taken for root initiatione0
- Direction
- minimume0
- Value
- 13.3 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days)”
t0“Jatropha curcas L.”
Claim · model-written summary
Fortified half MS medium produced 5.1 roots per shoot.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g ACe0
- Outcome
- number of roots per shootse0
- Direction
- maximume0
- Value
- 5.1e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1)”
t0“Jatropha curcas L.”
Claim · model-written summary
Fortified half MS medium produced longest roots of 4.9 cm.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g ACe0
- Outcome
- length of longest roote0
- Direction
- maximume0
- Value
- 4.9 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1) and length of longest root (4.9 cm)”
t0“Jatropha curcas L.”
Claim · model-written summary
Propagated plantlets showed nearly complete survival after hardening and acclimatization.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- nearly cent per cent survivale0
- Qualifier
- after hardening and acclimatizatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such produced plantlets showed nearly cent per cent survival after hardening and acclimatization”
t0“Jatropha curcas L.”
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