Abstract
Meloidogyne graminicola larvae immersed for 48 hours in 100% concentration of Paecilomyces lilacinus culture filtrate had 100% larval mortality while only 7.12% and 18.72% mortality was recorded, after 6 and 12 hours immersion, respectively. Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration. The culture filtrate also limited egg hatching as shown by lesser galls produced in rice seedling roots. The mycelial extract was found more potent compared with the culture filtrate extract; however, both fractions affected the larvae and eggs of M. graminicola. The dried ethyl acetate fraction gave 88.51% mortality at concentration of 500 mg/ml, followed by 76.91% at concentration of 400 mg/ml. A lethal concentration (LC) of 50% was attained at 300 mg/ml. Sixteen fractions were recovered from the crude dried extract of the fungus using vacuum liquid chromatography (VLC). Bioassay revealed that fraction 3 (combined fractions 5, 6 and 7) gave 39 percent nematode mortality suggesting that the nematoxic compounds are present in these fractions. The fragmentation pattern in GC-MS revealed that the active fraction contains more than 20 compounds with 5 major ones. The structure of the main compound was partially identified as derivative of azulene containing two unsaturation points. The limited amount of the fraction subjected to spectroscopic analysis does not warrant its purification to be able to do other analysis such as NMR spectroscopy for structure elucidation and complete compound identification.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Immersion of Meloidogyne graminicola larvae for 48 h in 100% Paecilomyces lilacinus culture filtrate caused 100% larval mortality, versus 7.12% and 18.72% after 6 and 12 h.
- Taxon
- rice
Oryza sativae3 - Treatment
- Paecilomyces lilacinus culture filtratee1
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae1pest
- Second interactor
- Paecilomyces lilacinus
Purpureocillium lilacinume1biocontrol_agent - Outcome
- larval mortalitye1
- Direction
- higherinferrede1
- Value
- 100%e1
- Comparator
- after 6 and 12 hours immersione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Meloidogyne graminicola larvae immersed for 48 hours in 100% concentration of Paecilomyces lilacinus culture filtrate had 100% larval mortality while only 7.12% and 18.72% mortality was recorded, after 6 and 12 hours immersion, respectively”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
Culture filtrate at 100% concentration produced no galls on rice seedlings.
- Taxon
- riceOryza sativae3
- Treatment
- culture filtratee3
- Outcome
- gallse3
- Direction
- decreaseinferrede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
Culture filtrate limited egg hatching of the nematode, shown by fewer galls on rice seedling roots.
- Taxon
- riceOryza sativae4
- Treatment
- culture filtratee4
- Outcome
- egg hatchinge4
- Direction
- limitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The culture filtrate also limited egg hatching as shown by lesser galls produced in rice seedling roots”
Claim · model-written summary
Mycelial extract was more potent than culture filtrate extract against M. graminicola larvae and eggs.
- Taxon
- riceOryza sativae3
- Treatment
- mycelial extracte5
- Interactor
- M. graminicolaMeloidogyne graminicolae5pest
- Outcome
- larvae and eggse5
- Direction
- more potente5
- Comparator
- culture filtrate extracte5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The mycelial extract was found more potent compared with the culture filtrate extract; however, both fractions affected the larvae and eggs of M. graminicola”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
Fraction 3 caused 39% nematode mortality in the bioassay.
- Taxon
- riceOryza sativae3
- Treatment
- fraction 3 (combined fractions 5, 6 and 7)e6
- Outcome
- nematode mortalitye6
- Value
- 39 percente6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Bioassay revealed that fraction 3 (combined fractions 5, 6 and 7) gave 39 percent nematode mortality suggesting that the nematoxic compounds are present in these fractions”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
The nematoxic compounds are suggested to be present in fraction 3 and fractions 5, 6 and 7.
- Taxon
- riceOryza sativae3
- Outcome
- nematoxic compoundse6
- Qualifier
- suggestinge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Bioassay revealed that fraction 3 (combined fractions 5, 6 and 7) gave 39 percent nematode mortality suggesting that the nematoxic compounds are present in these fractions”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
GC-MS of the active fraction showed more than 20 compounds, with 5 major ones.
- Taxon
- riceOryza sativae3
- Outcome
- compoundse7
- Value
- more than 20e7
- Qualifier
- with 5 major onese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“The fragmentation pattern in GC-MS revealed that the active fraction contains more than 20 compounds with 5 major ones”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
The main compound of the active fraction was partially identified as an azulene derivative with two unsaturation points.
- Taxon
- riceOryza sativae3
- Compound
- azulenee4
- Outcome
- structure of the main compounde4
- Qualifier
- partially identifiede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The structure of the main compound was partially identified as derivative of azulene containing two unsaturation points”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
Dried ethyl acetate fraction gave 88.51% mortality at 500 mg/ml.
- Taxon
- riceOryza sativae3
- Treatment
- dried ethyl acetate fractione4
- Outcome
- mortalitye4
- Value
- 88.51%e4
- Qualifier
- at concentration of 500 mg/mle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The dried ethyl acetate fraction gave 88.51% mortality at concentration of 500 mg/ml, followed by 76.91% at concentration of 400 mg/ml”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
Dried ethyl acetate fraction gave 76.91% mortality at 400 mg/ml.
- Taxon
- riceOryza sativae3
- Treatment
- dried ethyl acetate fractione4
- Outcome
- mortalitye4
- Value
- 76.91%e4
- Qualifier
- at concentration of 400 mg/mle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The dried ethyl acetate fraction gave 88.51% mortality at concentration of 500 mg/ml, followed by 76.91% at concentration of 400 mg/ml”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
Claim · model-written summary
A lethal concentration (LC50) was reached at 300 mg/ml.
- Taxon
- riceOryza sativae3
- Outcome
- lethal concentration (LC) of 50%e4
- Value
- 300 mg/mle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“A lethal concentration (LC) of 50% was attained at 300 mg/ml”
e3“Toxic metabolites present in the culture filtrate affected the larvae as there were no galls produced in rice seedlings at 100% concentration”
The same questions in other papers
pathogen product · pest · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- negative
pathogen product · Meloidogyne graminicola · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
- positive
- positive
botanical · Meloidogyne graminicola · Oryza sativa (rice)Results agree
10 papers in the same direction as this paper
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.