Abstract
The efl'eel of ammonium nitrate (an essential macrosalt) on plant regeneration from mesocotyl segments of indica rice (Oryza saliva Linn. cv. Safari-I7) has been investigated. Callus developed from the cut ends of mesocotyl segments on MS medium supplemented with 2,4-D (20 !J.M), BAP (2.2 J.l.M) and various concentrations of ammonium nitrate (10-80 mM). Highly organised and nodular callus developed after transfer of morphogenic calli to the MS medium containing 5 IJM 2,4-D, 2.21JM BAP and ammonium nitrate (20-80 mM). The presence of ammonium nitrate was found to be essential for the induction and proliferation of embryogenic callus. Shoot differentiation occurred after transfer of calli to the MS medium containing 10,20 (MS original), 40 and 80 mM ammonium nitrate alone or in combination with 8.8 IJM BAP. No shoot regeneration occurred in the absence of ammonium nitrate. The highest frequency of shoot regeneration and average number of shoots per callus clump was obtained on MS medium containing 40 mM ammonium nitrate. No significant difference in regeneration frequency and average number of plantlets per callus clump was observed when BAP (8.8 J.l.M) was incorporated in the regeneration medium containing various levels of ammonium nitrate. Regenerated shoots were rooted on MS medium containing 4.9 ~M IBA. Rooted plantlets were established to soil. The results show that ammonium nitrate play an important role in plant regeneration in indica rice.
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Claims 4
Claim · model-written summary
Ammonium nitrate was essential for induction and proliferation of embryogenic callus in indica rice.
- Taxon
- Oryza sativa Linn.
Oryza sativat0 - Material
- Safari-I7e0
- Treatment
- ammonium nitratee1
- Outcome
- induction and proliferation of embryogenic calluse1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa Linn.”
e0“indica rice (Oryza saliva Linn. cv. Safari-I7)”
e1“The presence of ammonium nitrate was found to be essential for the induction and proliferation of embryogenic callus”
Claim · model-written summary
No shoot regeneration occurred in the absence of ammonium nitrate.
- Taxon
- Oryza sativa Linn.Oryza sativat0
- Material
- Safari-I7e0
- Treatment
- ammonium nitratee2
- Outcome
- shoot regeneratione2
- Comparator
- absence of ammonium nitratee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa Linn.”
e0“indica rice (Oryza saliva Linn. cv. Safari-I7)”
e2“No shoot regeneration occurred in the absence of ammonium nitrate”
Claim · model-written summary
The highest frequency of shoot regeneration and shoots per callus clump was obtained on medium with 40 mM ammonium nitrate.
- Taxon
- Oryza sativa Linn.Oryza sativat0
- Material
- Safari-I7e0
- Treatment
- 40 mM ammonium nitratee3
- Outcome
- frequency of shoot regeneration and average number of shoots per callus clumpe3
- Direction
- higheste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa Linn.”
e0“indica rice (Oryza saliva Linn. cv. Safari-I7)”
e3“The highest frequency of shoot regeneration and average number of shoots per callus clump was obtained on MS medium containing 40 mM ammonium nitrate”
Claim · model-written summary
Adding BAP (8.8 µM) to media with various ammonium nitrate levels did not significantly change regeneration frequency or plantlet number.
- Taxon
- Oryza sativa Linn.Oryza sativat0
- Material
- Safari-I7e0
- Treatment
- BAP (8.8 J.l.M)e4
- Outcome
- regeneration frequency and average number of plantlets per callus clumpe4
- Direction
- no_changeinferrede4
- Comparator
- various levels of ammonium nitratee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa Linn.”
e0“indica rice (Oryza saliva Linn. cv. Safari-I7)”
e4“No significant difference in regeneration frequency and average number of plantlets per callus clump was observed when BAP (8.8 J.l.M) was incorporated in the regeneration medium containing various levels of ammonium nitrate”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.