Paper · 2015 · http://www.ijsrp.org/research-paper-0115/ijsrp-p3780.pdf

Effect of Waterlogging Stress on Growth Characteristics and Sod Gene Expression in Sugarcane

Shivam Bajpai; Rashmi Chandra

10
claims
22
verbatim quotes
8
citations
2
research areas
—
Not open access

Abstract

Abstract- Two sugarcane varieties, V1 (an early maturing) and V2 ( a mid-late maturing) were planted during spring season of 2014 in tray culture conditions at IMS Engineering college, Ghaziabad with an objective to study the effect of short term waterlogging on growth and physio-biochemical attributes, RNA isolation and expression of SOD (superoxide dismutase) using gene specific primer pairs. After about 60 days of planting, waterlogging stress treatment was imposed to plants by transferring planted pots in a bucket filled with water. Treatments consist of control 48, 96 hr. of waterlogging stress and recovery after 2 days of termination of treatments. Observations were recorded for SPAD Reading, RWC, chlorophylls and carotenoid contents, proline, and activity of SOD and peroxidase enzymes, soluble protein content and Cu-Zn-SOD gene expression. Results obtained indicated increased Relative water content, proline content and activity of SOD and peroxidase enzyme under waterlogging duration. An early maturing variety, V1 showed increased SPAD reading and higher accumulation of proline content than variety V2. In both varieties Chlorophylls and carotenoids contents decreased under waterlogging treatment. Due to waterlogging treatment, in variety V1 SOD gene expression was high at all duration level (0, 48, 96 hr. and recovery) as composed to control, while in V2 variety, treatment plants showed lower expression.

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Claims 10

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Waterlogging increased relative water content.

Taxon
Sugarcanet0
Treatment
waterlogging duratione0
Outcome
Relative water contente0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“increased Relative water content, proline content and activity of SOD and peroxidase enzyme under waterlogging duration”

✓ located verbatim in the source · abstract, characters 886–1005 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Waterlogging increased proline content.

Taxon
Sugarcanet0
Treatment
waterlogging duratione0
Outcome
proline contente0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“increased Relative water content, proline content and activity of SOD and peroxidase enzyme under waterlogging duration”

✓ located verbatim in the source · abstract, characters 886–1005 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Waterlogging increased SOD activity.

Taxon
Sugarcanet0
Treatment
waterlogging duratione0
Gene
SODe0
Outcome
activity of SODe0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“increased Relative water content, proline content and activity of SOD and peroxidase enzyme under waterlogging duration”

✓ located verbatim in the source · abstract, characters 886–1005 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Waterlogging increased peroxidase activity.

Taxon
Sugarcanet0
Treatment
waterlogging duratione0
Gene
peroxidasee0
Outcome
peroxidase enzymee0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“increased Relative water content, proline content and activity of SOD and peroxidase enzyme under waterlogging duration”

✓ located verbatim in the source · abstract, characters 886–1005 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

V1 had higher SPAD reading than V2.

Taxon
Sugarcanet0
Material
V1e0
Outcome
SPAD readinge0
Direction
increasede0
Comparator
variety V2e0

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“V1 showed increased SPAD reading and higher accumulation of proline content than variety V2”

✓ located verbatim in the source · abstract, characters 1034–1125 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

V1 accumulated more proline than V2.

Taxon
Sugarcanet0
Material
V1e0
Outcome
proline contente0
Direction
highere0
Comparator
variety V2e0

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“V1 showed increased SPAD reading and higher accumulation of proline content than variety V2”

✓ located verbatim in the source · abstract, characters 1034–1125 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Waterlogging decreased chlorophyll content in both varieties.

Taxon
Sugarcanet0
Treatment
waterlogging treatmente0
Outcome
Chlorophyllse0
Direction
decreasede0
Qualifier
both varietiese0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“In both varieties Chlorophylls and carotenoids contents decreased under waterlogging treatment”

✓ located verbatim in the source · abstract, characters 1127–1221 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Waterlogging decreased carotenoid content in both varieties.

Taxon
Sugarcanet0
Treatment
waterlogging treatmente0
Outcome
carotenoids contentse0
Direction
decreasede0
Qualifier
both varietiese0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“In both varieties Chlorophylls and carotenoids contents decreased under waterlogging treatment”

✓ located verbatim in the source · abstract, characters 1127–1221 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

V1 showed high SOD gene expression at reported durations and recovery relative to control.

Taxon
Sugarcanet0
Material
V1e0
Treatment
waterlogging treatmente1
Gene
SODe0
Outcome
SOD gene expressione0
Direction
highe0
Qualifier
all duration level (0, 48, 96 hr. and recovery)e0
Comparator
controle0

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“in variety V1 SOD gene expression was high at all duration level (0, 48, 96 hr. and recovery) as composed to control”

✓ located verbatim in the source · abstract, characters 1254–1370 · show in text ↑
e1

“Due to waterlogging treatment”

✓ located verbatim in the source · abstract, characters 1223–1252 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

V2 treatment plants showed lower SOD expression.

Taxon
Sugarcanet0
Material
V2e0
Treatment
treatment plantse0
Gene
SODe1
Outcome
expressione0
Direction
lowere0

Not reportedComparator

Verbatim

t0

“Sugarcane”

✓ located verbatim in the source · title, characters 83–92 · show in text ↑
e0

“in V2 variety, treatment plants showed lower expression”

✓ located verbatim in the source · abstract, characters 1378–1433 · show in text ↑
e1

“SOD gene expression was high at all duration level (0, 48, 96 hr. and recovery) as composed to control”

✓ located verbatim in the source · abstract, characters 1268–1370 · show in text ↑

flooding · molecular: metabolites · Saccharum officinarum (sugarcane)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

flooding · physiology: pigments · Saccharum officinarum (sugarcane)Results agree

4 papers: 0 positive, 4 negative or no effect

3 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.