Abstract
ARGONAUTE1 (AGO1) mediates microRNA- and small interfering RNA-directed posttranscriptional gene silencing in Arabidopsis thaliana. Mutant alleles of SQUINT (SQN) slightly reduce AGO1 activity and have weak effects on shoot morphology. A screen for mutations that suppress the sqn phenotype produced loss-of-function mutations in the Fbox gene FBW2. Mutations in FBW2 not only suppress sqn but also suppress many of the developmental phenotypes of weak, but not null, alleles of AGO1 by increasing AGO1 protein levels. Conversely, over-expression of FBW2 decreases the abundance of the AGO1 protein but not AGO1 messenger RNA, further indicating that FBW2 regulates AGO1 protein levels. fbw2 mutants have no obvious morphological phenotype, but display a reduced sensitivity to abscisic acid (ABA) that can be attributed to increased AGO1 activity. Our results indicate that FBW2 is a novel negative regulator of AGO1 and suggest that it plays a role in ABA signalling and/or response.
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Claims 11
Claim · model-written summary
Mutant SQN alleles slightly reduced AGO1 activity and weakly affected shoot morphology.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Gene
- SQUINT (SQN)e0
- Outcome
- AGO1 activity and have weak effects on shoot morphologye0
- Direction
- reducee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant alleles of SQUINT (SQN) slightly reduce AGO1 activity and have weak effects on shoot morphology”
t0“Arabidopsis thaliana”
Claim · model-written summary
A suppressor screen identified loss-of-function FBW2 mutations.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- FBW2e0
- Outcome
- loss-of-function mutations in the Fbox gene FBW2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A screen for mutations that suppress the sqn phenotype produced loss-of-function mutations in the Fbox gene FBW2”
t0“Arabidopsis thaliana”
Claim · model-written summary
FBW2 mutations suppressed the sqn phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutations in FBW2e0
- Gene
- FBW2e0
- Outcome
- sqne0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in FBW2 not only suppress sqn”
t0“Arabidopsis thaliana”
Claim · model-written summary
FBW2 mutations suppressed developmental phenotypes of weak but not null AGO1 alleles.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutations in FBW2e1
- Gene
- AGO1e0
- Outcome
- developmental phenotypese0
- Direction
- suppresse0
- Comparator
- null, alleles of AGO1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppress many of the developmental phenotypes of weak, but not null, alleles of AGO1 by increasing AGO1 protein levels”
e1“Mutations in FBW2”
t0“Arabidopsis thaliana”
Claim · model-written summary
FBW2 mutations increased AGO1 protein levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Mutations in FBW2e0
- Gene
- AGO1e0
- Outcome
- AGO1 protein levelse0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutations in FBW2 not only suppress sqn but also suppress many of the developmental phenotypes of weak, but not null, alleles of AGO1 by increasing AGO1 protein levels”
t0“Arabidopsis thaliana”
Claim · model-written summary
FBW2 over-expression decreased AGO1 protein abundance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- over-expression of FBW2e0
- Gene
- AGO1e0
- Outcome
- abundance of the AGO1 proteine0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of FBW2 decreases the abundance of the AGO1 protein”
t0“Arabidopsis thaliana”
Claim · model-written summary
FBW2 over-expression did not decrease AGO1 messenger RNA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- over-expression of FBW2e0
- Gene
- AGO1e0
- Outcome
- AGO1 messenger RNAe0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expression of FBW2 decreases the abundance of the AGO1 protein but not AGO1 messenger RNA”
t0“Arabidopsis thaliana”
Claim · model-written summary
fbw2 mutants had no obvious morphological phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- fbw2 mutantse0
- Outcome
- no obvious morphological phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fbw2 mutants have no obvious morphological phenotype”
t0“Arabidopsis thaliana”
Claim · model-written summary
fbw2 mutants had reduced sensitivity to ABA.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- fbw2 mutantse0
- Outcome
- sensitivity to abscisic acid (ABA)e0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fbw2 mutants have no obvious morphological phenotype, but display a reduced sensitivity to abscisic acid (ABA)”
t0“Arabidopsis thaliana”
Claim · model-written summary
Authors attribute reduced ABA sensitivity to increased AGO1 activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AGO1e0
- Outcome
- reduced sensitivity to abscisic acid (ABA)e0
- Qualifier
- increased AGO1 activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced sensitivity to abscisic acid (ABA) that can be attributed to increased AGO1 activity”
t0“Arabidopsis thaliana”
Claim · model-written summary
FBW2 is interpreted as a negative regulator of AGO1 with a possible role in ABA signaling or response.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- FBW2e0
- Outcome
- negative regulator of AGO1e0
- Qualifier
- ABA signalling and/or responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FBW2 is a novel negative regulator of AGO1 and suggest that it plays a role in ABA signalling and/or response”
t0“Arabidopsis thaliana”
The same questions in other papers
loss of function · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
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- negative
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See all 73 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.