Abstract
A nutrient film technique (NFT) culture system was developed to allow nursery production of arbuscular mycorrhizal horticultural crops. This would benefit horticultural production and allow for uncomplicated production of mycorrhizal hyphae. Roots of lettuce ( Lactuca sativa var. capitata ) plants were highly colonized by the arbuscular mycorrhizal fungus , Glomus mosseae (BEG 107) after 4 weeks in the NFT system, following an initial phase of five weeks in inoculated in Perlite substrate. In the NFT system, a thin layer of glass beads was used to provide solid support for plant and fungus growth and nutrient solution was supplied intermittently (15 min, six times per day). A modified nutrient solution (80 μ m P) was used and was replaced with fresh solution every 3 days. A significantly higher dry weight was found for the mycorrhizal versus the nonmycorrhizal lettuce plants in Perlite during the precolonization period. The root colonization rate was also high at rates up to 80 μ m P supply. On the NFT system, growth differences between mycorrhizal and nonmycorrhizal plants were less than in Perlite. However, root colonization rate was not reduced during the NFT culture period. In this system, high amounts of fungal biomass were produced. This would allow the determination of metal and other nutrient concentrations in fungal hyphae. Furthermore, we found large amounts of external fungal hyphae surrounding the root surface. As much as 130 mg fungal biomass were collected per culture plate (three plants). Therefore, we suggest that this modified NFT culture system would be suitable for fungal biomass production on a large scale with a view to additional aeration by intermittent nutrient supply, optimum P supply, and a use of glass beads as support materials. Furthermore, bulk inoculum composition with a mixture of spores, colonized roots, and hyphae grown in soilless media by the modified NFT system might be a useful way to mass-produce mycorrhizal crops and inoculum for commercial horticultural purposes.
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Claims 9
Claim · model-written summary
Lettuce roots were highly colonized by Glomus mosseae after four weeks in NFT following inoculated Perlite culture.
- Taxon
- lettuce
Lactuca sativae0 - Material
- Lactuca sativa var. capitatae0
- Organ / stage
- Rootse0
- Interactor
- Glomus mosseae (BEG 107)
Funneliformis mosseaee0symbiont - Outcome
- highly colonizede0
- Qualifier
- after 4 weeks in the NFT systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Roots of lettuce ( Lactuca sativa var. capitata ) plants were highly colonized by the arbuscular mycorrhizal fungus , Glomus mosseae (BEG 107) after 4 weeks in the NFT system, following an initial phase of five weeks in inoculated in Perlite substrate.”
Claim · model-written summary
Mycorrhizal lettuce had significantly higher dry weight than nonmycorrhizal lettuce in Perlite.
- Taxon
- lettuceLactuca sativae0
- Treatment
- mycorrhizale0
- Outcome
- dry weighte0
- Direction
- highere0
- Comparator
- nonmycorrhizal lettuce plantse0
- Setting
- Perlitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A significantly higher dry weight was found for the mycorrhizal versus the nonmycorrhizal lettuce plants in Perlite during the precolonization period.”
Claim · model-written summary
Root colonization remained high with phosphorus supply up to 80 μ m.
- Taxon
- lettuceLactuca sativae7
- Interactor
- Glomus mosseae (BEG 107)Funneliformis mosseaee6symbiont
- Outcome
- root colonization ratee0
- Direction
- highe0
- Value
- 80 μ m Pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The root colonization rate was also high at rates up to 80 μ m P supply.”
e7“Roots of lettuce ( Lactuca sativa var. capitata ) plants”
e6“Glomus mosseae (BEG 107)”
Claim · model-written summary
Growth differences between mycorrhizal and nonmycorrhizal lettuce were smaller in NFT than Perlite.
- Taxon
- lettuceLactuca sativae7
- Outcome
- growth differencese0
- Direction
- lesse0
- Comparator
- Perlitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the NFT system, growth differences between mycorrhizal and nonmycorrhizal plants were less than in Perlite.”
e7“Roots of lettuce ( Lactuca sativa var. capitata ) plants”
Claim · model-written summary
Root colonization was not reduced during NFT culture.
- Taxon
- lettuceLactuca sativae7
- Interactor
- Glomus mosseae (BEG 107)Funneliformis mosseaee6symbiont
- Outcome
- root colonization ratee0
- Direction
- not reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root colonization rate was not reduced during the NFT culture period.”
e7“Roots of lettuce ( Lactuca sativa var. capitata ) plants”
e6“Glomus mosseae (BEG 107)”
Claim · model-written summary
NFT culture produced high fungal biomass, including external hyphae around lettuce roots.
- Taxon
- lettuceLactuca sativae7
- Interactor
- Glomus mosseae (BEG 107)Funneliformis mosseaee6symbiont
- Outcome
- fungal biomasse0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this system, high amounts of fungal biomass were produced. This would allow the determination of metal and other nutrient concentrations in fungal hyphae. Furthermore, we found large amounts of external fungal hyphae surrounding the root surface.”
e7“Roots of lettuce ( Lactuca sativa var. capitata ) plants”
e6“Glomus mosseae (BEG 107)”
Claim · model-written summary
Up to 130 mg fungal biomass was collected per culture plate.
- Taxon
- lettuceLactuca sativae7
- Interactor
- Glomus mosseae (BEG 107)Funneliformis mosseaee6symbiont
- Outcome
- fungal biomasse0
- Value
- 130 mge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As much as 130 mg fungal biomass were collected per culture plate (three plants).”
e7“Roots of lettuce ( Lactuca sativa var. capitata ) plants”
e6“Glomus mosseae (BEG 107)”
Claim · model-written summary
Authors recommend modified NFT culture for large scale fungal biomass production.
- Taxon
- lettuceLactuca sativae7
- Treatment
- modified NFT culture systeme0
- Outcome
- fungal biomass production on a large scalee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we suggest that this modified NFT culture system would be suitable for fungal biomass production on a large scale with a view to additional aeration by intermittent nutrient supply, optimum P supply, and a use of glass beads as support materials.”
e7“Roots of lettuce ( Lactuca sativa var. capitata ) plants”
Claim · model-written summary
Authors suggest mixed spores, roots and hyphae from NFT culture may mass produce mycorrhizal crops and inoculum.
Verbatim
e0“bulk inoculum composition with a mixture of spores, colonized roots, and hyphae grown in soilless media by the modified NFT system might be a useful way to mass-produce mycorrhizal crops and inoculum for commercial horticultural purposes.”
The same questions in other papers
mycorrhiza · growth · Lactuca sativa (garden lettuce)Positive and negative results
23 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative
- positive
- no effect
- no effect
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.