Abstract
Quantitative resistance is polygenically controlled and durable, but the underlying molecular and biochemical mechanisms are poorly understood. Secondary cell wall thickening is a critical process in quantitative resistance, regulated by transcriptional networks. This paper provides compelling evidence on the functionality of StWRKY1 transcription factor, in a compatible interaction of potato-Phytophthora infestans, to extend our knowledge on the regulation of the metabolic pathway genes leading to strengthening the secondary cell wall. A metabolomics approach was used to identify resistance-related metabolites belonging to the phenylpropanoid pathway and their biosynthetic genes regulated by StWRKY1. The StWRKY1 gene in resistant potato was silenced to decipher its role in the regulation of phenylpropanoid pathway genes to strengthen the secondary cell wall. Sequencing of the promoter region of StWRKY1 in susceptible genotypes revealed the absence of heat shock elements (HSEs). Simultaneous induction of both the heat shock protein (sHSP17.8) and StWRKY1 following pathogen invasion enables functioning of the latter to interact with the HSE present in the resistant StWRKY1 promoter region. EMSA and luciferase transient expression assays further revealed direct binding of StWRKY1 to promoters of hydroxycinnamic acid amide (HCAA) biosynthetic genes encoding 4-coumarate:CoA ligase and tyramine hydroxycinnamoyl transferase. Silencing of the StWRKY1 gene was associated with signs of reduced late blight resistance by significantly increasing the pathogen biomass and decreasing the abundance of HCAAs. This study provides convincing evidence on the role of StWRKY1 in the regulation of downstream genes to biosynthesize HCAAs, which are deposited to reinforce secondary cell walls.
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Claims 6
Claim · model-written summary
Susceptible potato genotypes lacked heat shock elements in the StWRKY1 promoter.
- Taxon
- potato
Solanum tuberosumt0 - Material
- susceptible genotypese0
- Gene
- StWRKY1e0
- Outcome
- absence of heat shock elements (HSEs)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequencing of the promoter region of StWRKY1 in susceptible genotypes revealed the absence of heat shock elements (HSEs)”
t0“potato”
Claim · model-written summary
Pathogen invasion induced sHSP17.8 and StWRKY1 together.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- Phytophthora infestans
Phytophthora infestanst0pathogen - Gene
- sHSP17.8e0
- Outcome
- Simultaneous induction of both the heat shock protein (sHSP17.8) and StWRKY1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Simultaneous induction of both the heat shock protein (sHSP17.8) and StWRKY1 following pathogen invasion”
t0“potato-Phytophthora infestans”
Claim · model-written summary
StWRKY1 bound promoters of genes encoding 4-coumarate:CoA ligase and tyramine hydroxycinnamoyl transferase.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StWRKY1e0
- Outcome
- direct binding of StWRKY1 to promoters of hydroxycinnamic acid amide (HCAA) biosynthetic genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“direct binding of StWRKY1 to promoters of hydroxycinnamic acid amide (HCAA) biosynthetic genes encoding 4-coumarate:CoA ligase and tyramine hydroxycinnamoyl transferase”
t0“potato”
Claim · model-written summary
Silencing StWRKY1 significantly increased pathogen biomass in potato.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Silencing of the StWRKY1 genee0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogen
- Gene
- StWRKY1e0
- Outcome
- pathogen biomasse0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of the StWRKY1 gene was associated with signs of reduced late blight resistance by significantly increasing the pathogen biomass and decreasing the abundance of HCAAs”
t0“potato-Phytophthora infestans”
Claim · model-written summary
Silencing StWRKY1 decreased HCAA abundance in potato.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- Silencing of the StWRKY1 genee0
- Gene
- StWRKY1e0
- Outcome
- abundance of HCAAse0
- Direction
- decreasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of the StWRKY1 gene was associated with signs of reduced late blight resistance by significantly increasing the pathogen biomass and decreasing the abundance of HCAAs”
t0“potato”
Claim · model-written summary
StWRKY1 regulates downstream genes that synthesize HCAAs deposited to reinforce secondary cell walls.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- StWRKY1e0
- Outcome
- reinforce secondary cell wallse0
- Qualifier
- HCAAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StWRKY1 in the regulation of downstream genes to biosynthesize HCAAs, which are deposited to reinforce secondary cell walls”
t0“potato”
The same questions in other papers
loss of function · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
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