Abstract
A cDNA encoding a chitinase-related receptor-like kinase, designated CHRK1, was isolated from tobacco (Nicotiana tabacum). The C-terminal kinase domain (KD) of CHRK1 contained all of the conserved amino acids of serine/threonine protein kinases. The putative extracellular domain was closely related to the class V chitinase of tobacco and to microbial chitinases. CHRK1 mRNA accumulation was strongly stimulated by infection with fungal pathogen and tobacco mosaic virus. Amino acid-sequence analysis revealed that the chitinase-like domain of CHRK1 lacked the essential glutamic acid residue required for chitinase activity. The recombinant chitinase-like domain did not show any catalytic activity for either oligomeric or polymeric chitin substrates. The recombinant KD of CHRK1 exhibited autophosphorylation, but the mutant KD with a mutation in the essential ATP-binding site did not, suggesting that CHRK1 encoded a functional kinase. CHRK1 was detected in membrane fractions of tobacco BY2 cells. Furthermore, CHRK1-GFP fusion protein was localized in plasma membranes when it was expressed in animal cells. This is the first report of a new type of receptor-like kinase containing a chitinase-like sequence in the putative extracellular domain.
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Claims 10
Claim · model-written summary
A cDNA encoding CHRK1 was isolated from tobacco.
- Taxon
- tobacco (Nicotiana tabacum)
Nicotiana tabacume0 - Gene
- CHRK1e0
- Outcome
- A cDNA encoding a chitinase-related receptor-like kinasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A cDNA encoding a chitinase-related receptor-like kinase, designated CHRK1, was isolated from tobacco (Nicotiana tabacum)”
Claim · model-written summary
The CHRK1 kinase domain contained conserved amino acids of serine/threonine protein kinases.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- CHRK1e0
- Outcome
- conserved amino acids of serine/threonine protein kinasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The C-terminal kinase domain (KD) of CHRK1 contained all of the conserved amino acids of serine/threonine protein kinases”
t0“Tobacco”
Claim · model-written summary
The putative extracellular domain resembled tobacco class V chitinase and microbial chitinases.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- closely related to the class V chitinase of tobacco and to microbial chitinasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The putative extracellular domain was closely related to the class V chitinase of tobacco and to microbial chitinases”
Claim · model-written summary
Fungal pathogen infection strongly stimulated CHRK1 mRNA accumulation.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- infection with fungal pathogene0
- Interactor
- fungal pathogene0pathogen
- Gene
- CHRK1e0
- Outcome
- CHRK1 mRNA accumulatione0
- Direction
- strongly stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CHRK1 mRNA accumulation was strongly stimulated by infection with fungal pathogen and tobacco mosaic virus”
t0“Tobacco”
Claim · model-written summary
Tobacco mosaic virus infection strongly stimulated CHRK1 mRNA accumulation.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- tobacco mosaic viruse0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Gene
- CHRK1e0
- Outcome
- CHRK1 mRNA accumulatione0
- Direction
- strongly stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CHRK1 mRNA accumulation was strongly stimulated by infection with fungal pathogen and tobacco mosaic virus”
t0“Tobacco”
Claim · model-written summary
The CHRK1 chitinase-like domain lacked the glutamic acid residue required for chitinase activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- CHRK1e0
- Outcome
- lacked the essential glutamic acid residue required for chitinase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the chitinase-like domain of CHRK1 lacked the essential glutamic acid residue required for chitinase activity”
t0“Tobacco”
Claim · model-written summary
The recombinant CHRK1 chitinase-like domain had no catalytic activity with oligomeric or polymeric chitin.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- CHRK1t0
- Outcome
- catalytic activity for either oligomeric or polymeric chitin substratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant chitinase-like domain did not show any catalytic activity for either oligomeric or polymeric chitin substrates”
t0“CHRK1, a Chitinase-Related Receptor-Like Kinase in Tobacco”
Claim · model-written summary
The recombinant CHRK1 kinase domain autophosphorylated, whereas its ATP-binding-site mutant did not.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- mutant KDe0
- Gene
- CHRK1e0
- Outcome
- autophosphorylatione0
- Direction
- did note0
- Comparator
- mutant KDe0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant KD of CHRK1 exhibited autophosphorylation, but the mutant KD with a mutation in the essential ATP-binding site did not”
t0“Tobacco”
Claim · model-written summary
CHRK1 was found in membrane fractions of tobacco BY2 cells.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- BY2 cellse0
- Gene
- CHRK1e0
- Outcome
- detected in membrane fractionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CHRK1 was detected in membrane fractions of tobacco BY2 cells”
Claim · model-written summary
CHRK1-GFP fusion protein localized in plasma membranes when expressed in animal cells.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- CHRK1-GFPe0
- Outcome
- localized in plasma membranese0
- Qualifier
- expressed in animal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CHRK1-GFP fusion protein was localized in plasma membranes when it was expressed in animal cells”
t0“Tobacco”
The same questions in other papers
infection · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
16 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.