Abstract
Starch phosphorylasel (Pho1) from etiolated rice (Oryza sativa L. cv. Tainong 67) seedlings was purified by ammonium sulfate fractionation, DEAE-Sepharose CL-6B anion exchange chromatography, and dextrin-Sepharose 4B affinity chromatography. The purification fold was 299, and the enzyme activity recovery was about 21%. The molecular mass of the native Pho1 on Superose 12 gel filtration was 145 kDa. The subunit molecular weight as determined by SDS-PAGE was 85 kDa. The enzyme has an optimum pH of 5 and an optimum reaction temperature of about 45℃~50℃. In the synthetic reaction for Glc 1-P, the k(subscript m) value was 2.1 mM, and the V(subscript max) value was 5.85 U mg ^-1. In the phosphorolytic direction for orthophosphate, the k(subscript m) value was 3.8 mM. Phol has a higher affinity for amylopectin, glycogen, soluble starch and dextrin than for maltooligosaccharide (6 to 10 glucose units). In addition, the k (subscript m) value for amylopectin was ninefold lower than for dextrin. Cyclohexaamylose, cycloheptaamylose, cyclooctaamylose, and maltotetrose were inhibitors of Pho1. Mannose 1-P, Fru 6-P, ADPGlc, UDPGlc, AMP, IMP and PEP also inhibit Pho1. The metal ions Ag (superscript +), Hg(superscript 2+), and Zn(superscript 2+) also reduce the enzyme activity. However, thiol reagents activate Phol activity, suggesting that sulfhydryl-group(s) may be required for enzyme stability.
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Claims 19
Claim · model-written summary
Pho1 purification was 299-fold with about 21% activity recovery.
- Taxon
- rice
Oryza sativae0 - Outcome
- purification folde1
- Value
- 299e1
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“etiolated rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“The purification fold was 299, and the enzyme activity recovery was about 21%”
Claim · model-written summary
Pho1 enzyme activity recovery was about 21%.
- Taxon
- riceOryza sativae0
- Outcome
- enzyme activity recoverye1
- Value
- about 21%e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“the enzyme activity recovery was about 21%”
Claim · model-written summary
Native Pho1 had mass 145 kDa.
- Taxon
- riceOryza sativae0
- Gene
- Pho1e1
- Outcome
- molecular masse1
- Value
- 145 kDae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“The molecular mass of the native Pho1 on Superose 12 gel filtration was 145 kDa”
Claim · model-written summary
Pho1 subunit mass was 85 kDa.
- Taxon
- riceOryza sativae0
- Outcome
- subunit molecular weighte1
- Value
- 85 kDae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“The subunit molecular weight as determined by SDS-PAGE was 85 kDa”
Claim · model-written summary
The enzyme optimum pH was 5.
- Taxon
- riceOryza sativae0
- Outcome
- optimum pHe1
- Value
- 5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“The enzyme has an optimum pH of 5”
Claim · model-written summary
The enzyme optimum temperature was about 45℃~50℃.
- Taxon
- riceOryza sativae0
- Outcome
- optimum reaction temperaturee1
- Value
- about 45℃~50℃e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“an optimum reaction temperature of about 45℃~50℃”
Claim · model-written summary
Synthetic Glc 1-P reaction k(subscript m) was 2.1 mM.
- Taxon
- riceOryza sativae0
- Outcome
- k(subscript m) valuee1
- Value
- 2.1 mMe1
- Qualifier
- synthetic reaction for Glc 1-Pe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“In the synthetic reaction for Glc 1-P, the k(subscript m) value was 2.1 mM”
Claim · model-written summary
Synthetic reaction V(subscript max) was 5.85 U mg ^-1.
- Taxon
- riceOryza sativae0
- Outcome
- V(subscript max) valuee1
- Value
- 5.85 U mg ^-1e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“the V(subscript max) value was 5.85 U mg ^-1”
Claim · model-written summary
Orthophosphate phosphorolytic k(subscript m) was 3.8 mM.
- Taxon
- riceOryza sativae0
- Outcome
- k(subscript m) valuee1
- Value
- 3.8 mMe1
- Qualifier
- phosphorolytic direction for orthophosphatee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“In the phosphorolytic direction for orthophosphate, the k(subscript m) value was 3.8 mM”
Claim · model-written summary
Pho1 affinity was higher for amylopectin, glycogen, soluble starch and dextrin than for maltooligosaccharide.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“Phol has a higher affinity for amylopectin, glycogen, soluble starch and dextrin than for maltooligosaccharide (6 to 10 glucose units)”
Claim · model-written summary
The amylopectin k value was ninefold lower than dextrin.
- Taxon
- riceOryza sativae0
- Outcome
- k (subscript m) valuee1
- Direction
- lowere1
- Value
- ninefolde1
- Comparator
- dextrine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“the k (subscript m) value for amylopectin was ninefold lower than for dextrin”
Claim · model-written summary
Cyclohexaamylose, cycloheptaamylose, and cyclooctaamylose inhibited Pho1.
- Taxon
- riceOryza sativae0
- Treatment
- Cyclohexaamylose, cycloheptaamylose, cyclooctaamylosee1
- Outcome
- Pho1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“Cyclohexaamylose, cycloheptaamylose, cyclooctaamylose, and maltotetrose were inhibitors of Pho1”
Claim · model-written summary
Maltotetrose inhibited Pho1.
- Taxon
- riceOryza sativae0
- Treatment
- maltotetrosee1
- Outcome
- Pho1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“maltotetrose were inhibitors of Pho1”
Claim · model-written summary
Mannose 1-P, Fru 6-P, and ADPGlc inhibited Pho1.
- Taxon
- riceOryza sativae0
- Treatment
- Mannose 1-P, Fru 6-P, ADPGlce1
- Outcome
- Pho1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“Mannose 1-P, Fru 6-P, ADPGlc, UDPGlc, AMP, IMP and PEP also inhibit Pho1”
Claim · model-written summary
UDPGlc, AMP, and IMP inhibited Pho1.
- Taxon
- riceOryza sativae0
- Treatment
- UDPGlc, AMP, IMPe1
- Outcome
- Pho1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“UDPGlc, AMP, IMP and PEP also inhibit Pho1”
Claim · model-written summary
PEP inhibited Pho1.
- Taxon
- riceOryza sativae0
- Treatment
- PEPe1
- Outcome
- Pho1e1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“PEP also inhibit Pho1”
Claim · model-written summary
Silver, mercury, and zinc ions reduced enzyme activity.
- Taxon
- riceOryza sativae0
- Treatment
- Ag (superscript +), Hg(superscript 2+), and Zn(superscript 2+)e1
- Outcome
- enzyme activitye1
- Direction
- reducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“The metal ions Ag (superscript +), Hg(superscript 2+), and Zn(superscript 2+) also reduce the enzyme activity”
Claim · model-written summary
Thiol reagents activated Phol activity.
- Taxon
- riceOryza sativae0
- Treatment
- thiol reagentse1
- Outcome
- Phol activitye1
- Direction
- activatee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“thiol reagents activate Phol activity”
Claim · model-written summary
Sulfhydryl groups may be required for enzyme stability.
- Taxon
- riceOryza sativae0
- Outcome
- enzyme stabilitye1
- Qualifier
- sulfhydryl-group(s)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa L. cv. Tainong 67) seedlings”
e1“suggesting that sulfhydryl-group(s) may be required for enzyme stability”
The same questions in other papers
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See all 12 papers →silver atom · soil: biology · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.