Abstract
Hydrogen peroxide (H(2)O(2)) and nitric oxide (*NO) are key reactive species in signal transduction pathways leading to activation of plant defense against biotic or abiotic stress. Here, we investigated the effect of pre-treating citrus plants (Citrus aurantium L.) with either of these two molecules on plant acclimation to salinity and show that both pre-treatments strongly reduced the detrimental phenotypical and physiological effects accompanying this stress. A proteomic analysis disclosed 85 leaf proteins that underwent significant quantitative variations in plants directly exposed to salt stress. A large part of these changes was not observed with salt-stressed plants pre-treated with either H(2)O(2) or sodium nitroprusside (SNP; a *NO-releasing chemical). We also identified several proteins undergoing changes either in their oxidation (carbonylation; 40 proteins) and/or S-nitrosylation (49 proteins) status in response to salinity stress. Both H(2)O(2) and SNP pre-treatments before salinity stress alleviated salinity-induced protein carbonylation and shifted the accumulation levels of leaf S-nitrosylated proteins to those of unstressed control plants. Altogether, the results indicate an overlap between H(2)O(2)- and *NO-signaling pathways in acclimation to salinity and suggest that the oxidation and S-nitrosylation patterns of leaf proteins are specific molecular signatures of citrus plant vigour under stressful conditions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Hydrogen peroxide pretreatment reduced detrimental phenotypic and physiological effects of salinity.
- Taxon
- Citrus aurantium L.
Citrus ×aurantiume0 - Treatment
- pre-treating citrus plants (Citrus aurantium L.) with either of these two moleculese0
- Outcome
- detrimental phenotypical and physiological effectse0
- Direction
- reducede0
- Comparator
- salinitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-treating citrus plants (Citrus aurantium L.) with either of these two molecules on plant acclimation to salinity and show that both pre-treatments strongly reduced the detrimental phenotypical and physiological effects accompanying this stress”
Claim · model-written summary
Salt stress significantly changed 85 leaf proteins.
- Treatment
- salt stresse0
- Outcome
- leaf proteins that underwent significant quantitative variationse0
- Value
- 85 leaf proteinse0
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“85 leaf proteins that underwent significant quantitative variations in plants directly exposed to salt stress”
t0“citrus plants”
Claim · model-written summary
Pretreatment with peroxide or SNP prevented many salt-induced protein changes.
- Treatment
- H(2)O(2) or sodium nitroprussidee0
- Outcome
- these changese0
- Direction
- not observede0
- Comparator
- salt-stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A large part of these changes was not observed with salt-stressed plants pre-treated with either H(2)O(2) or sodium nitroprusside (SNP; a *NO-releasing chemical)”
t0“citrus plants”
Claim · model-written summary
Salinity altered carbonylation status of 40 proteins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“changes either in their oxidation (carbonylation; 40 proteins) and/or S-nitrosylation (49 proteins) status in response to salinity stress”
t0“citrus plants”
Claim · model-written summary
Salinity altered S-nitrosylation status of 49 proteins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S-nitrosylation (49 proteins) status in response to salinity stress”
t0“citrus plants”
Claim · model-written summary
Peroxide and SNP pretreatment alleviated salt-induced protein carbonylation.
- Treatment
- H(2)O(2) and SNP pre-treatmentse0
- Outcome
- salinity-induced protein carbonylatione0
- Direction
- alleviatede0
- Comparator
- salinity stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both H(2)O(2) and SNP pre-treatments before salinity stress alleviated salinity-induced protein carbonylation”
t0“citrus plants”
Claim · model-written summary
Peroxide and SNP pretreatment shifted S-nitrosylated leaf protein accumulation toward unstressed levels.
- Treatment
- H(2)O(2) and SNP pre-treatmentse0
- Outcome
- accumulation levels of leaf S-nitrosylated proteinse0
- Direction
- shiftede0
- Comparator
- unstressed control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both H(2)O(2) and SNP pre-treatments before salinity stress alleviated salinity-induced protein carbonylation and shifted the accumulation levels of leaf S-nitrosylated proteins to those of unstressed control plants”
t0“citrus plants”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · CitrusPositive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.