Abstract
One of the water stress-specific cDNA clones of rice characterised previously, wsi18, was selected for further study. The wsi18 gene can be induced by water stress conditions such as mannitol, NaCl, and dryness, but not by ABA, cold, or heat. A genomic clone for wsi18, pwsi18, contained about 1.7 kbp of the 5' upstream sequence, two introns, and the full coding sequence. The 5'-upstream sequence of pwsi18 contained putative cis-acting elements, namely an ABA-responsive element (ABRE), three G-boxes, three E-boxes, a MEF-2 sequence, four direct and two inverted repeats, and four sequences similar to DRE, which is involved in the dehydration response of Arabidopsis genes. The gusA reporter gene under the control of the pwsi18 promoter showed transient expression in response to water stress. Deletion of the downstream DRE-like sequence between the distal G-boxes-2 and -3 resulted in rather low GUS expression.
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Claims 6
Claim · model-written summary
wsi18 was induced by mannitol, NaCl and dryness.
- Taxon
- Rice
Oryza sativat0 - Treatment
- water stress conditions such as mannitol, NaCl, and drynesse0
- Gene
- wsi18e0
- Outcome
- inducede0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The wsi18 gene can be induced by water stress conditions such as mannitol, NaCl, and dryness”
t0“Rice”
Claim · model-written summary
wsi18 was not induced by ABA, cold or heat.
- Taxon
- RiceOryza sativat0
- Treatment
- ABA, cold, or heate0
- Outcome
- not by ABA, cold, or heate0
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not by ABA, cold, or heat”
t0“Rice”
Claim · model-written summary
The pwsi18 genomic clone contains upstream sequence, two introns and a full coding sequence.
- Taxon
- RiceOryza sativat0
- Gene
- pwsi18e0
- Outcome
- 5' upstream sequence, two introns, and the full coding sequencee0
- Value
- 1.7 kbpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A genomic clone for wsi18, pwsi18, contained about 1.7 kbp of the 5' upstream sequence, two introns, and the full coding sequence”
t0“Rice”
Claim · model-written summary
The pwsi18 upstream region contained multiple putative cis elements.
- Taxon
- RiceOryza sativat0
- Gene
- pwsi18e0
- Outcome
- putative cis-acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 5'-upstream sequence of pwsi18 contained putative cis-acting elements, namely an ABA-responsive element (ABRE), three G-boxes, three E-boxes, a MEF-2 sequence, four direct and two inverted repeats, and four sequences similar to DRE”
t0“Rice”
Claim · model-written summary
The pwsi18 promoter drove transient gusA expression under water stress.
- Taxon
- RiceOryza sativat0
- Treatment
- water stresse0
- Gene
- gusAe0
- Outcome
- transient expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gusA reporter gene under the control of the pwsi18 promoter showed transient expression in response to water stress”
t0“Rice”
Claim · model-written summary
Deleting the downstream DRE-like sequence yielded low GUS expression.
- Taxon
- RiceOryza sativat0
- Treatment
- Deletion of the downstream DRE-like sequencee0
- Gene
- GUSe0
- Outcome
- GUS expressione0
- Direction
- lowe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Deletion of the downstream DRE-like sequence between the distal G-boxes-2 and -3 resulted in rather low GUS expression”
t0“Rice”
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