Abstract
Plant dehydroascorbate reductase (DHAR), which re-reduces oxidized ascorbate to maintain an appropriate level of ascorbate, is very important, but no gene or cDNA for plant DHAR has been cloned yet. Here, we describe a cDNA for a rice glutathione-dependent DHAR (designated DHAR1). A recombinant Dhar1p produced in Escherichia coli was functional. The expression sequence tag database suggests that Dhar1p homologs exist in various plants. Furthermore, the rice Dhar1p has a low similarity to rat DHAR, although the rice enzyme has a considerably higher specific activity than the mammalian one. The mRNA level of DHAR1, the protein level of Dhar1p and the DHAR activity in rice seedlings were elevated by high temperature, suggesting the protection role of DHAR at high temperature.
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Claims 8
Claim · model-written summary
A cDNA for rice glutathione-dependent DHAR was described.
- Taxon
- rice
Oryza sativae0 - Gene
- DHAR1e0
- Outcome
- a cDNA for a rice glutathione-dependent DHARe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we describe a cDNA for a rice glutathione-dependent DHAR (designated DHAR1).”
Claim · model-written summary
Recombinant Dhar1p made in Escherichia coli was functional.
- Taxon
- riceOryza sativat0
- Gene
- Dhar1pe0
- Outcome
- functionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A recombinant Dhar1p produced in Escherichia coli was functional.”
t0“rice”
Claim · model-written summary
Database analysis suggested Dhar1p homologs occur in various plants.
Verbatim
e0“The expression sequence tag database suggests that Dhar1p homologs exist in various plants.”
Claim · model-written summary
Rice Dhar1p showed low similarity to rat DHAR.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice Dhar1p has a low similarity to rat DHAR”
Claim · model-written summary
The rice enzyme had higher specific activity than the mammalian one.
- Taxon
- riceOryza sativae0
- Outcome
- specific activitye0
- Direction
- highere0
- Comparator
- the mammalian onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice enzyme has a considerably higher specific activity than the mammalian one”
Claim · model-written summary
High temperature elevated DHAR1 mRNA in rice seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Treatment
- high temperaturee0
- Gene
- DHAR1e0
- Outcome
- mRNA level of DHAR1e0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mRNA level of DHAR1, the protein level of Dhar1p and the DHAR activity in rice seedlings were elevated by high temperature”
Claim · model-written summary
High temperature elevated Dhar1p protein in rice seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Treatment
- high temperaturee0
- Gene
- Dhar1pe0
- Outcome
- protein level of Dhar1pe0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mRNA level of DHAR1, the protein level of Dhar1p and the DHAR activity in rice seedlings were elevated by high temperature”
Claim · model-written summary
High temperature elevated DHAR activity in rice seedlings.
- Taxon
- riceOryza sativae0
- Organ / stage
- seedlingse0
- Treatment
- high temperaturee0
- Outcome
- DHAR activitye0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mRNA level of DHAR1, the protein level of Dhar1p and the DHAR activity in rice seedlings were elevated by high temperature”
The same questions in other papers
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See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.