Abstract
Transgenic soybean (Glycine max) culture cells expressing apoaequorin, a Ca2+ indicator, were exposed to glucan fragments derived from Phytophthora sojae or to chitin oligomers. The effects of these elicitors on cytosolic Ca2+ concentrations and on mRNA levels of two beta-tubulin isoforms, tubB1 and tubB2, were investigated. The glucan elicitors, to which the cells are known to react with a biphasic cytosolic Ca2+ increase, induced a down-regulation of the tubB1 mRNA levels while the tubB2 mRNA level remained constant. The decrease of tubB1 mRNA level was observed after 1 hour of glucan treatment. In contrast, chitin oligomers, known to provoke a monophasic Ca2+ increase of short duration, did not affect the tubB1 mRNA level. Pre-incubation with 10 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, an extracellular Ca2+ chelator, blocked the cytosolic Ca2+ increase as well as the decrease of tubB1 mRNA levels induced by glucan elicitors. Likewise, pre-incubation with 1 mM neomycin, which reduced only the second glucan-induced Ca2+ peak, blocked the decrease of tubB1 mRNA level. Experiments with cordycepin, a transcription inhibitor, indicated that glucan fragments induced the degradation of tubB1 mRNA. In conclusion, the glucan-induced cytosolic Ca2+ changes are correlated with a strong increase in tubB1 mRNA degradation.
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Claims 11
Claim · model-written summary
Glucan elicitors caused a biphasic cytosolic calcium increase.
- Taxon
- soybean
Glycine maxt0 - Treatment
- glucan elicitorse0
- Interactor
- Phytophthora sojaePhytophthora sojaee1pathogen
- Outcome
- cytosolic Ca2+e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucan elicitors, to which the cells are known to react with a biphasic cytosolic Ca2+ increase”
t0“Soybean”
e1“Phytophthora sojae”
Claim · model-written summary
Glucan elicitors down-regulated tubB1 mRNA after one hour.
- Taxon
- soybeanGlycine maxt0
- Treatment
- glucan treatmente0
- Interactor
- Phytophthora sojaePhytophthora sojaee1pathogen
- Gene
- tubB1e0
- Outcome
- tubB1 mRNA levele0
- Direction
- decreasee0
- Qualifier
- after 1 houre0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decrease of tubB1 mRNA level was observed after 1 hour of glucan treatment”
t0“Soybean”
e1“Phytophthora sojae”
Claim · model-written summary
tubB2 mRNA remained constant after glucan elicitation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- glucan elicitorse1
- Interactor
- Phytophthora sojaePhytophthora sojaee2pathogen
- Gene
- tubB2e0
- Outcome
- tubB2 mRNA levele0
- Direction
- remained constante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the tubB2 mRNA level remained constant”
t0“Soybean”
e1“The glucan elicitors”
e2“Phytophthora sojae”
Claim · model-written summary
Chitin oligomers caused a short monophasic calcium increase.
- Taxon
- soybeanGlycine maxt0
- Treatment
- chitin oligomerse0
- Outcome
- Ca2+e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chitin oligomers, known to provoke a monophasic Ca2+ increase of short duration”
t0“Soybean”
Claim · model-written summary
Chitin oligomers did not affect tubB1 mRNA.
- Taxon
- soybeanGlycine maxt0
- Treatment
- chitin oligomerse0
- Gene
- tubB1e0
- Outcome
- tubB1 mRNA levele0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“chitin oligomers, known to provoke a monophasic Ca2+ increase of short duration, did not affect the tubB1 mRNA level”
t0“Soybean”
Claim · model-written summary
The extracellular calcium chelator blocked the glucan-induced calcium increase.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 10 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acide0
- Outcome
- cytosolic Ca2+ increasee0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-incubation with 10 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, an extracellular Ca2+ chelator, blocked the cytosolic Ca2+ increase as well as the decrease of tubB1 mRNA levels induced by glucan elicitors”
t0“Soybean”
Claim · model-written summary
The extracellular calcium chelator blocked the glucan-induced tubB1 mRNA decrease.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 10 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acide0
- Gene
- tubB1e0
- Outcome
- decrease of tubB1 mRNA levelse0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-incubation with 10 mM 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, an extracellular Ca2+ chelator, blocked the cytosolic Ca2+ increase as well as the decrease of tubB1 mRNA levels induced by glucan elicitors”
t0“Soybean”
Claim · model-written summary
Neomycin reduced only the second glucan-induced calcium peak.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 1 mM neomycine0
- Outcome
- second glucan-induced Ca2+ peake0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-incubation with 1 mM neomycin, which reduced only the second glucan-induced Ca2+ peak”
t0“Soybean”
Claim · model-written summary
Neomycin blocked the glucan-induced decline in tubB1 mRNA.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 1 mM neomycine0
- Gene
- tubB1e0
- Outcome
- decrease of tubB1 mRNA levele0
- Direction
- blockede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-incubation with 1 mM neomycin, which reduced only the second glucan-induced Ca2+ peak, blocked the decrease of tubB1 mRNA level”
t0“Soybean”
Claim · model-written summary
Glucan fragments induced tubB1 mRNA degradation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- glucan fragmentse0
- Gene
- tubB1e0
- Outcome
- degradation of tubB1 mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucan fragments induced the degradation of tubB1 mRNA”
t0“Soybean”
Claim · model-written summary
Glucan-induced cytosolic calcium changes correlated with greater tubB1 mRNA degradation.
- Taxon
- soybeanGlycine maxt0
- Gene
- tubB1e0
- Compound
- Ca2+e0
- Outcome
- tubB1 mRNA degradatione0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucan-induced cytosolic Ca2+ changes are correlated with a strong increase in tubB1 mRNA degradation”
t0“Soybean”
The same questions in other papers
resistance inducer · molecular: expression · Glycine max (soybean)Positive and negative results
8 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →resistance inducer · Phytophthora sojae · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.