Abstract
To investigate the mechanisms underlying activation of plant defenses against microbial attack we have studied elicitor regulation of a chimeric gene comprising the 5' flanking region of a defense gene encoding the phytoalexin biosynthetic enzyme chalcone synthase fused to a bacterial chloramphenicol acetyltransferase gene. Glutathione or fungal elicitor caused a rapid, marked but transient expression of the chimeric gene electroporated into soybean protoplasts. The response closely resembled that of endogenous chalcone synthase genes in suspension cultured cells. Functional analysis of 5' deletions suggests that promoter activity is determined by an elicitor-regulated activator located between the "TATA box" and nucleotide position -173 and an upstream silencer between -173 and -326. These cis-acting elements function in the transduction of the elicitation signal to initiate elaboration of an inducible defense response.
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Claims 6
Claim · model-written summary
Glutathione rapidly and transiently induced chimeric gene expression in soybean protoplasts.
- Taxon
- soybean
Glycine maxe0 - Treatment
- Glutathionee0
- Outcome
- expression of the chimeric genee0
- Direction
- causede0
- Qualifier
- rapid, marked but transiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutathione or fungal elicitor caused a rapid, marked but transient expression of the chimeric gene electroporated into soybean protoplasts”
Claim · model-written summary
Fungal elicitor rapidly and transiently induced chimeric gene expression in soybean protoplasts.
- Taxon
- soybeanGlycine maxe0
- Treatment
- fungal elicitore0
- Outcome
- expression of the chimeric genee0
- Direction
- causede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutathione or fungal elicitor caused a rapid, marked but transient expression of the chimeric gene electroporated into soybean protoplasts”
Claim · model-written summary
The chimeric response resembled endogenous chalcone synthase gene responses.
- Taxon
- soybeanGlycine maxe1
- Gene
- chalcone synthasee0
- Outcome
- response closely resembled that of endogenous chalcone synthase genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The response closely resembled that of endogenous chalcone synthase genes in suspension cultured cells”
e1“soybean protoplasts”
Claim · model-written summary
Promoter activity depends on an elicitor-regulated activator between the TATA box and position -173.
- Taxon
- soybeanGlycine maxe1
- Outcome
- promoter activitye0
- Qualifier
- elicitor-regulated activator located between the "TATA box" and nucleotide position -173e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoter activity is determined by an elicitor-regulated activator located between the "TATA box" and nucleotide position -173”
e1“soybean protoplasts”
Claim · model-written summary
Promoter activity depends on an upstream silencer between -173 and -326.
- Taxon
- soybeanGlycine maxe2
- Outcome
- promoter activitye1
- Qualifier
- upstream silencer between -173 and -326e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an upstream silencer between -173 and -326”
e1“promoter activity is determined”
e2“soybean protoplasts”
Claim · model-written summary
These cis-acting elements transduce the elicitation signal to initiate defense.
- Taxon
- soybeanGlycine maxe1
- Outcome
- inducible defense responsee0
- Qualifier
- transduction of the elicitation signale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These cis-acting elements function in the transduction of the elicitation signal to initiate elaboration of an inducible defense response”
e1“soybean protoplasts”
The same questions in other papers
resistance inducer · molecular: expression · Glycine max (soybean)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.