Abstract
The study was undertaken to develop an efficient protocol for in vitro regeneration of shallot (Allium cepa). Two local shallot varieties (Huruta and Minjar) were used as experimental materials where basal discs were used as explants. Murashige and Skoog medium supplemented with different concentrations and combinations of 2, 4-Dichlorophenoxyacetic Acid, 6-Benzylaminopurine, Kinetin and α-naphthaleneacetic acid were used for callus induction and regeneration of plantlet. Maximum callus induction was observed in genotype Huruta (81.11%) in medium supplemented with 1mg/l 2, 4- Dichlorophenoxyacetic Acid. In combined effect both genotypes Huruta and Minjar showed highest callus induction (74.44%) from basal discs placed in medium supplemented with 1 mg/1 2,4- Dichlorophenoxyacetic Acid. Among the different types and combination of plant growth regulators, the maximum callus fresh weight of 1.26 and 1.20 g were achieved with 1 mg/l 2, 4- Dichlorophenoxyacetic Acid and α-naphthaleneacetic acid combined with 1 mg/l 6-Benzylaminopurine, respectively. Regenerated plants were obtained via somatic embryogenesis and organogenesis. Murashige and Skoog medium supplemented with 5.0 mg/l 6-Benzylaminopurine + 0.1 mg/l α-naphthaleneacetic acid showed higher percentage of shoot regeneration (91.11%). 1.5 mg/l indole-3-butyric acid + 2 mg/l 6-Benzylaminopurine was the optimum concentration giving 86.66% of rooted plantlets. The survival rate of transferred regenerated plantlets was satisfactory 66.6% and 60.0% for Minjar and Huruta respectively.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Huruta reached 81.11% maximum callus induction with 2,4-D.
- Taxon
- shallot
Allium cepa var. aggregatumt0 - Material
- Hurutae0
- Treatment
- 1mg/l 2, 4- Dichlorophenoxyacetic Acide0
- Outcome
- callus inductione0
- Direction
- Maximume0
- Value
- 81.11%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum callus induction was observed in genotype Huruta (81.11%) in medium supplemented with 1mg/l 2, 4- Dichlorophenoxyacetic Acid.”
t0“shallot”
Claim · model-written summary
Huruta and Minjar reached 74.44% callus induction with 2,4-D.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Material
- Huruta and Minjare0
- Organ / stage
- basal discse0
- Treatment
- 1 mg/1 2,4- Dichlorophenoxyacetic Acide0
- Outcome
- callus inductione0
- Direction
- higheste0
- Value
- 74.44%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both genotypes Huruta and Minjar showed highest callus induction (74.44%) from basal discs placed in medium supplemented with 1 mg/1 2,4- Dichlorophenoxyacetic Acid.”
t0“shallot”
Claim · model-written summary
The 2,4-D medium gave maximum callus fresh weight of 1.26 g.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Treatment
- 1 mg/l 2, 4- Dichlorophenoxyacetic Acide0
- Outcome
- callus fresh weighte0
- Direction
- maximume0
- Value
- 1.26e0
- Qualifier
- ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum callus fresh weight of 1.26 and 1.20 g were achieved with 1 mg/l 2, 4- Dichlorophenoxyacetic Acid and α-naphthaleneacetic acid combined with 1 mg/l 6-Benzylaminopurine, respectively.”
t0“shallot”
Claim · model-written summary
The NAA plus BAP medium gave maximum callus fresh weight of 1.20 g.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Treatment
- α-naphthaleneacetic acid combined with 1 mg/l 6-Benzylaminopurinee0
- Outcome
- callus fresh weighte0
- Direction
- maximume0
- Value
- 1.20 ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum callus fresh weight of 1.26 and 1.20 g were achieved with 1 mg/l 2, 4- Dichlorophenoxyacetic Acid and α-naphthaleneacetic acid combined with 1 mg/l 6-Benzylaminopurine, respectively.”
t0“shallot”
Claim · model-written summary
Regenerated plants arose through somatic embryogenesis and organogenesis.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Outcome
- Regenerated plants were obtained via somatic embryogenesis and organogenesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regenerated plants were obtained via somatic embryogenesis and organogenesis.”
t0“shallot”
Claim · model-written summary
BAP plus NAA gave 91.11% shoot regeneration.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Treatment
- 5.0 mg/l 6-Benzylaminopurine + 0.1 mg/l α-naphthaleneacetic acide0
- Outcome
- shoot regeneratione0
- Direction
- highere0
- Value
- 91.11%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Murashige and Skoog medium supplemented with 5.0 mg/l 6-Benzylaminopurine + 0.1 mg/l α-naphthaleneacetic acid showed higher percentage of shoot regeneration (91.11%).”
t0“shallot”
Claim · model-written summary
IBA plus BAP gave 86.66% rooted plantlets.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Treatment
- 1.5 mg/l indole-3-butyric acid + 2 mg/l 6-Benzylaminopurinee0
- Outcome
- rooted plantletse0
- Direction
- optimume0
- Value
- 86.66%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1.5 mg/l indole-3-butyric acid + 2 mg/l 6-Benzylaminopurine was the optimum concentration giving 86.66% of rooted plantlets.”
t0“shallot”
Claim · model-written summary
Transferred Minjar plantlets survived at 66.6%.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Material
- Minjare0
- Outcome
- survival rate of transferred regenerated plantletse0
- Direction
- satisfactorye0
- Value
- 66.6%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The survival rate of transferred regenerated plantlets was satisfactory 66.6% and 60.0% for Minjar and Huruta respectively.”
t0“shallot”
Claim · model-written summary
Transferred Huruta plantlets survived at 60.0%.
- Taxon
- shallotAllium cepa var. aggregatumt0
- Material
- Hurutae0
- Outcome
- survival rate of transferred regenerated plantletse0
- Direction
- satisfactorye0
- Value
- 60.0%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The survival rate of transferred regenerated plantlets was satisfactory 66.6% and 60.0% for Minjar and Huruta respectively.”
t0“shallot”
The same questions in other papers
N-benzyladenine · growth · Allium cepa (onion)Results differ
13 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →1-naphthaleneacetic acid · growth · Allium cepa (onion)Positive and negative results
9 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- negative
9 papers more in this group
See all 13 papers →indole-3-butyric acid · growth · Allium cepa (onion)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.