Paper · 2009 · Hereditas

Effects of 6-(3-methyl-2-buten-1-ylamino)-purine and α-naphthaleneacetic acid on root formation and cytology of root tips and callus in tissue cultures of Allium cepa var. proliferum

SHAKUNTALA NANDI; GUNNAR FRIDBORG; TAGE ERIKSSON

5
claims
10
verbatim quotes
16
citations
1
research areas
✓
Open access

Abstract

Tissue cultures of Allium cepa var. proliferum grown in liquid medium were treated with a cytokinin, 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and α-naphthaleneacetic acid (NAA) in different concentrations. The formation and elongation of roots were stimulated by 2 iP, the optimum concentration of which varied with the age of the cultures. The mixoploid root tips of the control series showed a high percentage of polyploidy, but with 2 iP in the medium the cells were essentially 100% diploid. A similar effect was seen in cells of callus tissue. The effects of NAA were similar to those of cytokinin in that the root tip cells showed increasing diploidy to nearly 100% at the optimum concentration of 5 mg/l. However, in the NAA-treated series root formation and elongation were less pronounced than in the 2 iP-treated series.

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Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

2 iP stimulated root formation and elongation in tissue cultures, with an optimum concentration that varied with culture age

Taxon
Allium cepa var. proliferumAllium cepae1
Treatment
2 iPe1
Outcome
formation and elongation of rootse2
Direction
stimulatede2
Qualifier
the optimum concentration of which varied with the age of the culturese2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Tissue cultures of Allium cepa var. proliferum grown in liquid medium were treated with a cytokinin, 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and α-naphthaleneacetic acid (NAA) in different concentrations”

✓ located verbatim in the source · abstract, characters 0–208 · show in text ↑
e2

“The formation and elongation of roots were stimulated by 2 iP, the optimum concentration of which varied with the age of the cultures”

✓ located verbatim in the source · abstract, characters 210–343 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

NAA gave less pronounced root formation and elongation than 2 iP

Taxon
Allium cepa var. proliferumAllium cepae1
Treatment
NAAe1
Outcome
root formation and elongatione3
Direction
less pronouncede3
Comparator
2 iP-treated seriese3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Tissue cultures of Allium cepa var. proliferum grown in liquid medium were treated with a cytokinin, 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and α-naphthaleneacetic acid (NAA) in different concentrations”

✓ located verbatim in the source · abstract, characters 0–208 · show in text ↑
e3

“However, in the NAA-treated series root formation and elongation were less pronounced than in the 2 iP-treated series”

✓ located verbatim in the source · abstract, characters 716–833 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

With 2 iP, root-tip cells were essentially 100% diploid, whereas controls showed a high percentage of polyploidy

Taxon
Allium cepa var. proliferumAllium cepae1
Organ / stage
root tipse4
Treatment
2 iPe4
Outcome
percentage of polyploidye4
Direction
decreaseinferrede4
Value
100%e4
Comparator
control seriese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Tissue cultures of Allium cepa var. proliferum grown in liquid medium were treated with a cytokinin, 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and α-naphthaleneacetic acid (NAA) in different concentrations”

✓ located verbatim in the source · abstract, characters 0–208 · show in text ↑
e4

“The mixoploid root tips of the control series showed a high percentage of polyploidy, but with 2 iP in the medium the cells were essentially 100% diploid”

✓ located verbatim in the source · abstract, characters 345–498 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

2 iP had a similar effect on ploidy of callus tissue cells

Taxon
Allium cepa var. proliferumAllium cepae1
Organ / stage
cells of callus tissuee5
Treatment
2 iPe1
Outcome
similar effecte5

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Tissue cultures of Allium cepa var. proliferum grown in liquid medium were treated with a cytokinin, 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and α-naphthaleneacetic acid (NAA) in different concentrations”

✓ located verbatim in the source · abstract, characters 0–208 · show in text ↑
e5

“A similar effect was seen in cells of callus tissue”

✓ located verbatim in the source · abstract, characters 500–551 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

NAA increased root-tip cell diploidy to nearly 100% at 5 mg/l, similar to cytokinin

Taxon
Allium cepa var. proliferumAllium cepae1
Organ / stage
root tip cellse6
Treatment
NAAe1
Outcome
diploidye6
Direction
increasinge6
Value
nearly 100%e6
Qualifier
at the optimum concentration of 5 mg/le6
Comparator
cytokinine6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Tissue cultures of Allium cepa var. proliferum grown in liquid medium were treated with a cytokinin, 6-(3-methyl-2-buten-1-ylamino)-purine (2 iP) and α-naphthaleneacetic acid (NAA) in different concentrations”

✓ located verbatim in the source · abstract, characters 0–208 · show in text ↑
e6

“The effects of NAA were similar to those of cytokinin in that the root tip cells showed increasing diploidy to nearly 100% at the optimum concentration of 5 mg/l”

✓ located verbatim in the source · abstract, characters 553–714 · show in text ↑

1-naphthaleneacetic acid · growth · Allium cepa (onion)Positive and negative results

13 papers: 10 positive, 3 negative or no effect

2 papers in the same direction as this paper · 10 in the opposite direction

  1. positive
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.