Abstract
Simultaneous silencing of asparagine synthetase (Ast)-1 and -2 limits asparagine (ASN) formation and, consequently, reduces the acrylamide-forming potential of tubers. The phenotype of silenced lines appears normal in the greenhouse, but field-grown tubers are small and cracked. Assessing the effects of silencing StAst1 and StAst2 individually, we found that yield drag was mainly linked to down-regulation of StAst2. Interestingly, tubers from untransformed scions grafted onto intragenic StAst1/2-silenced rootstock contained almost the same low ASN levels as those in the original silenced lines, indicating that ASN is mainly formed in tubers rather than being transported from leaves. This conclusion was further supported by the finding that overexpression of StAst2 caused ASN to accumulate in leaves but not tubers. Thus, ASN does not appear to be the main form of organic nitrogen transported from leaves to tubers. Because reduced ASN levels coincided with increased levels of glutamine, it appears likely that this alternative amide amino acid is mobilized to tubers, where it is converted into ASN by StAst1. Indeed, tuber-specific silencing of StAst1, but not of StAst2, was sufficient to substantially lower ASN formation in tubers. Extensive field studies demonstrated that the reduced acrylamide-forming potential achieved by tuber-specific StAst1 silencing did not affect the yield or quality of field-harvested tubers.
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Claims 12
Claim · model-written summary
Simultaneous silencing of asparagine synthetase-1 and -2 reduces the acrylamide-forming potential of potato tubers.
- Taxon
- potatoes
Solanum tuberosumt0 - Organ / stage
- tuberse0
- Treatment
- Simultaneous silencinge0
- Gene
- asparagine synthetase (Ast)-1 and -2e0
- Compound
- asparagine (ASN)e0
- Outcome
- acrylamide-forming potentiale0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“Simultaneous silencing of asparagine synthetase (Ast)-1 and -2 limits asparagine (ASN) formation and, consequently, reduces the acrylamide-forming potential of tubers”
Claim · model-written summary
Field-grown tubers of silenced potato lines are small and cracked, although the greenhouse phenotype appears normal.
- Taxon
- potatoesSolanum tuberosumt0
- Organ / stage
- field-grown tuberse1
- Treatment
- silenced linese1
- Outcome
- small and crackede1
- Setting
- fielde1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e1“The phenotype of silenced lines appears normal in the greenhouse, but field-grown tubers are small and cracked”
Claim · model-written summary
Yield drag in potato was mainly linked to down-regulation of StAst2.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- down-regulation of StAst2e2
- Gene
- StAst2e2
- Outcome
- yield drage2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e2“yield drag was mainly linked to down-regulation of StAst2”
Claim · model-written summary
Tubers from untransformed scions grafted onto StAst1/2-silenced rootstock contained almost the same low ASN levels as the original silenced lines.
- Taxon
- potatoesSolanum tuberosumt0
- Organ / stage
- tuberse3
- Treatment
- grafted onto intragenic StAst1/2-silenced rootstocke3
- Outcome
- ASN levelse3
- Direction
- no_changeinferrede3
- Comparator
- original silenced linese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e3“tubers from untransformed scions grafted onto intragenic StAst1/2-silenced rootstock contained almost the same low ASN levels as those in the original silenced lines”
Claim · model-written summary
ASN is mainly formed in potato tubers rather than being transported from leaves.
- Taxon
- potatoesSolanum tuberosumt0
- Outcome
- ASN is mainly formed in tuberse4
- Qualifier
- rather than being transported from leavese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e4“ASN is mainly formed in tubers rather than being transported from leaves”
Claim · model-written summary
Overexpression of StAst2 caused ASN to accumulate in leaves.
- Taxon
- potatoesSolanum tuberosumt0
- Organ / stage
- leavese5
- Treatment
- overexpression of StAst2e5
- Outcome
- ASNe5
- Direction
- accumulatee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e5“overexpression of StAst2 caused ASN to accumulate in leaves but not tubers”
Claim · model-written summary
Overexpression of StAst2 did not cause ASN to accumulate in tubers.
- Taxon
- potatoesSolanum tuberosumt0
- Organ / stage
- tuberse5
- Treatment
- overexpression of StAst2e5
- Outcome
- ASNe5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e5“overexpression of StAst2 caused ASN to accumulate in leaves but not tubers”
Claim · model-written summary
Reduced ASN levels in potato coincided with increased levels of glutamine.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- reduced ASN levelse7
- Compound
- glutaminee7
- Outcome
- levels of glutaminee7
- Direction
- increasede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e7“reduced ASN levels coincided with increased levels of glutamine”
Claim · model-written summary
Glutamine is likely mobilised to potato tubers, where StAst1 converts it into ASN.
- Taxon
- potatoesSolanum tuberosumt0
- Gene
- StAst1e1
- Compound
- glutaminee0
- Outcome
- mobilized to tuberse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“reduced ASN levels coincided with increased levels of glutamine”
e1“this alternative amide amino acid is mobilized to tubers, where it is converted into ASN by StAst1”
Claim · model-written summary
ASN does not appear to be the main form of organic nitrogen transported from leaves to tubers in potato.
- Taxon
- potatoesSolanum tuberosumt0
- Outcome
- main form of organic nitrogene6
- Qualifier
- transported from leaves to tuberse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e6“ASN does not appear to be the main form of organic nitrogen transported from leaves to tubers”
Claim · model-written summary
Tuber-specific silencing of StAst1 substantially lowers ASN formation in potato tubers, whereas silencing of StAst2 does not.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- tuber-specific silencing of StAst1e0
- Gene
- StAst1e0
- Outcome
- ASN formation in tuberse0
- Direction
- lowere0
- Comparator
- StAst2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“tuber-specific silencing of StAst1, but not of StAst2, was sufficient to substantially lower ASN formation in tubers”
Claim · model-written summary
Tuber-specific StAst1 silencing, which lowers acrylamide-forming potential, did not affect yield or quality of field-harvested potato tubers.
- Taxon
- potatoesSolanum tuberosumt0
- Treatment
- tuber-specific StAst1 silencinge0
- Outcome
- yield or quality of field-harvested tuberse0
- Direction
- did not affecte0
- Setting
- fielde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“potatoes”
e0“the reduced acrylamide-forming potential achieved by tuber-specific StAst1 silencing did not affect the yield or quality of field-harvested tubers”
The same questions in other papers
loss of function · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
8 papers in the same direction as this paper · 13 in the opposite direction
- positive
- negative
- negative
18 papers more in this group
See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.