Abstract
Suberin is a cell wall lipid polyester found in the cork cells of the periderm offering protection against dehydration and pathogens. Its biosynthesis and assembly, as well as its contribution to the sealing properties of the periderm, are still poorly understood. Here, we report on the isolation of the coding sequence CYP86A33 and the molecular and physiological function of this gene in potato (Solanum tuberosum) tuber periderm. CYP86A33 was down-regulated in potato plants by RNA interference-mediated silencing. Periderm from CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 omega-hydroxyacid (approximately 70%) and alpha,omega-diacid (approximately 90%) monomers in comparison with wild type. Moreover, the glycerol esterified to suberin was reduced by 60% in the silenced plants. The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared and the thickness of the suberin layer was clearly reduced. In addition, the water permeability of the periderm isolated from CYP86A33-silenced lines was 3.5 times higher than that of the wild type. Thus, our data provide convincing evidence for the involvement of omega-functional fatty acids in establishing suberin structure and function.
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Claims 8
Claim · model-written summary
CYP86A33 silencing reduced aliphatic suberin load by 60%.
- Taxon
- potato
Solanum tuberosume1 - Organ / stage
- periderme0
- Treatment
- CYP86A33-silencede0
- Gene
- CYP86A33e0
- Outcome
- aliphatic suberin loade0
- Direction
- decreasee0
- Value
- 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Periderm from CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load”
e1“in potato (Solanum tuberosum) tuber periderm”
Claim · model-written summary
Silencing reduced C18:1 omega hydroxyacid monomers by approximately 70%.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- CYP86A33-silencede0
- Gene
- CYP86A33e0
- Outcome
- C18:1 omega-hydroxyacide0
- Direction
- reducede0
- Value
- approximately 70%e0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 omega-hydroxyacid (approximately 70%) and alpha,omega-diacid (approximately 90%) monomers in comparison with wild type”
e1“in potato (Solanum tuberosum) tuber periderm”
Claim · model-written summary
Silencing reduced alpha,omega diacid monomers by approximately 90%.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- CYP86A33-silencede2
- Gene
- CYP86A33e2
- Outcome
- alpha,omega-diacide0
- Direction
- reducede0
- Value
- approximately 90%e0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“greatly reduced levels of C18:1 omega-hydroxyacid (approximately 70%) and alpha,omega-diacid (approximately 90%) monomers in comparison with wild type”
e1“in potato (Solanum tuberosum) tuber periderm”
e2“CYP86A33-silenced plants”
Claim · model-written summary
Silencing reduced glycerol esterified to suberin by 60%.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- silenced plantse0
- Gene
- CYP86A33e2
- Outcome
- glycerol esterified to suberine0
- Direction
- reducede0
- Value
- 60%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the glycerol esterified to suberin was reduced by 60% in the silenced plants”
e1“in potato (Solanum tuberosum) tuber periderm”
e2“CYP86A33-silenced plants”
Claim · model-written summary
Silencing disrupted regular suberin lamellae.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- CYP86A33-silencede2
- Outcome
- typical regular ultrastructure of suberine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared”
e1“in potato (Solanum tuberosum) tuber periderm”
e2“CYP86A33-silenced plants”
Claim · model-written summary
Silencing reduced suberin layer thickness.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- CYP86A33-silencede2
- Outcome
- thickness of the suberin layere0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the thickness of the suberin layer was clearly reduced”
e1“in potato (Solanum tuberosum) tuber periderm”
e2“CYP86A33-silenced plants”
Claim · model-written summary
Silenced periderm had 3.5 times higher water permeability than wild type.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- CYP86A33-silenced linese0
- Outcome
- water permeability of the periderme0
- Direction
- highere0
- Value
- 3.5 timese0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the water permeability of the periderm isolated from CYP86A33-silenced lines was 3.5 times higher than that of the wild type”
e1“in potato (Solanum tuberosum) tuber periderm”
Claim · model-written summary
The findings implicate omega functional fatty acids in suberin structure and function.
- Taxon
- potatoSolanum tuberosume1
- Compound
- omega-functional fatty acidse0
- Outcome
- suberin structure and functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“evidence for the involvement of omega-functional fatty acids in establishing suberin structure and function”
e1“in potato (Solanum tuberosum) tuber periderm”
The same questions in other papers
loss of function · molecular: metabolites · Solanum tuberosum (potato)Positive and negative results
12 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.