Paper · 1985 · New Phytologist

INTERVENTION BY GIBBERELLIN AND CYTOKININ IN THE RELEASE OF APPLE EMBRYOS FROM DORMANCY: A REAPPRAISAL

C. BULARD

9
claims
21
verbatim quotes
13
citations
3
research areas
✓
Open access

Abstract

S ummary Embryos of Pyrus malus L. cv. Golden delicious, were isolated from the fruits and cultivated in aseptic conditions on solid media. Rate of cotyledon greening, evolution of germination percentage and growth of organs after germination were used to determine the extent of dormancy. Treatment with gibberellins and by chilling both induced an increase in the rate of cotyledon greening, in germination percentage as well as stimulating root growth. However, even the most active gibberellins, GA 4 , GA 7 , GA 9 , applied simultaneously at high concentration (5 mg l −1 ), could not completely reproduce the effects of optimum chilling treatment (9 to 12 weeks at 5 °C). While progressive increases in germination percentages were found with duration (chilling) or concentration (gibberellins), there was no direct relationship between concentration and response in the case of kinetin. Low concentrations (10 or 100 ug l −1 ) were inactive, whereas concentrations equal to or greater than 1 mg l −1 induced large increases in germination percentage. The concentrations which stimulated germination also inhibited growth, especially of the root.

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Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Gibberellins increased cotyledon greening, germination and root growth.

Taxon
Pyrus malus L.Malus domesticae1
Material
Golden deliciouse1
Treatment
gibberellinse0
Outcome
rate of cotyledon greening, in germination percentage as well as stimulating root growthe0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Treatment with gibberellins and by chilling both induced an increase in the rate of cotyledon greening, in germination percentage as well as stimulating root growth.”

✓ located verbatim in the source · abstract, characters 290–455 · show in text ↑
e1

“Embryos of Pyrus malus L. cv. Golden delicious”

✓ located verbatim in the source · abstract, characters 9–55 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chilling increased cotyledon greening, germination and root growth.

Taxon
Pyrus malus L.Malus domesticae1
Treatment
chillinge0
Outcome
rate of cotyledon greening, in germination percentage as well as stimulating root growthe0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Treatment with gibberellins and by chilling both induced an increase in the rate of cotyledon greening, in germination percentage as well as stimulating root growth.”

✓ located verbatim in the source · abstract, characters 290–455 · show in text ↑
e1

“Embryos of Pyrus malus L. cv. Golden delicious”

✓ located verbatim in the source · abstract, characters 9–55 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Even the most active gibberellins did not fully reproduce optimum chilling effects.

Treatment
GA 4 , GA 7 , GA 9 , applied simultaneously at high concentration (5 mg l −1 )e0
Outcome
effectse0
Direction
could not completely reproducee0
Comparator
optimum chilling treatmente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“even the most active gibberellins, GA 4 , GA 7 , GA 9 , applied simultaneously at high concentration (5 mg l −1 ), could not completely reproduce the effects of optimum chilling treatment (9 to 12 weeks at 5 °C)”

✓ located verbatim in the source · abstract, characters 465–676 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Germination increased progressively with chilling duration.

Treatment
chillinge0
Outcome
germination percentagese0
Direction
increasese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“progressive increases in germination percentages were found with duration (chilling)”

✓ located verbatim in the source · abstract, characters 684–768 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Germination increased progressively with gibberellin concentration.

Treatment
gibberellinse0
Outcome
germination percentagese0
Direction
increasese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“progressive increases in germination percentages were found with duration (chilling) or concentration (gibberellins)”

✓ located verbatim in the source · abstract, characters 684–800 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Kinetin concentration had no direct concentration-response relationship.

Treatment
kinetine0
Outcome
responsee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“there was no direct relationship between concentration and response in the case of kinetin”

✓ located verbatim in the source · abstract, characters 802–892 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Low kinetin concentrations were inactive for germination.

Treatment
Low concentrations (10 or 100 ug l −1 )e0
Outcome
germination percentagee1
Direction
inactivee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Low concentrations (10 or 100 ug l −1 ) were inactive”

✓ located verbatim in the source · abstract, characters 894–947 · show in text ↑
e1

“Rate of cotyledon greening, evolution of germination percentage”

✓ located verbatim in the source · abstract, characters 140–203 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Kinetin at 1 mg l−1 or greater greatly increased germination.

Treatment
kinetine1
Outcome
germination percentagee0
Direction
increasese0
Value
1 mg l −1e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“concentrations equal to or greater than 1 mg l −1 induced large increases in germination percentage”

✓ located verbatim in the source · abstract, characters 957–1056 · show in text ↑
e1

“in the case of kinetin”

✓ located verbatim in the source · abstract, characters 870–892 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Kinetin concentrations that stimulated germination inhibited growth, especially root growth.

Treatment
kinetine1
Outcome
growth, especially of the roote0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The concentrations which stimulated germination also inhibited growth, especially of the root.”

✓ located verbatim in the source · abstract, characters 1058–1152 · show in text ↑
e1

“in the case of kinetin”

✓ located verbatim in the source · abstract, characters 870–892 · show in text ↑
t0

“APPLE EMBRYOS”

✓ located verbatim in the source · title, characters 60–73 · show in text ↑

kinetin · growth · Malus domestica (apple)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. negative
  3. no effect

3 papers more in this group

See all 7 papers →

cold · growth · Malus domestica (apple)Positive and negative results

19 papers: 16 positive, 3 negative or no effect

15 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. negative

15 papers more in this group

See all 19 papers →

class · growth · Malus domestica (apple)Positive and negative results

46 papers: 38 positive, 8 negative or no effect

37 papers in the same direction as this paper · 8 in the opposite direction

  1. negative
  2. negative
  3. no effect

42 papers more in this group

See all 46 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.