Abstract
Nitric oxide (NO) is a signaling molecule that plays a critical role in the activation of innate immune and inflammatory responses in animals. During the last few years, NO has also been detected in several plant species and the increasing number of reports on its function in plants have implicated NO as an important effector of growth, development and defense. Analogously to animals, NO has been recently shown to inhibit tobacco aconitase. This suggests that NO may elevate free iron levels in the cells by converting tobacco cytoplasmic aconitase into a mRNA binding protein that negatively regulates accumulation of ferritin. We investigated the possible role of NO as a regulator of ferritin levels in Arabidopsis and found that the NO-donor sodium nitroprusside (SNP) induces accumulation of ferritin both at mRNA and protein level. Iron is not necessary for this NO-mediated ferritin transcript accumulation, since SNP is still able to induce the accumulation of ferritin transcript in Arabidopsis suspension cultures pre-treated with the iron chelants DFO or ferrozine. However, NO is required for iron-induced ferritin accumulation, as the NO scavenger CPTIO prevents ferritin transcript accumulation in Arabidopsis suspension cultures treated with iron. The pathway is ser/thr phosphatase-dependent and necessitates protein synthesis; furthermore, NO mediates ferritin regulation through the IDRS sequence of the Atfer1 promoter responsible for transcriptional repression under low iron supply. NO, by acting downstream of iron in the induction of ferritin transcript accumulation is therefore a key signaling molecule for regulation of iron homeostasis in plants.
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Claims 5
Claim · model-written summary
SNP induced ferritin mRNA and protein accumulation.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- sodium nitroprusside (SNP)e0
- Gene
- ferritine0
- Outcome
- accumulation of ferritin both at mRNA and protein levele0
- Direction
- induces accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NO-donor sodium nitroprusside (SNP) induces accumulation of ferritin both at mRNA and protein level”
t0“Arabidopsis”
Claim · model-written summary
Iron chelation did not prevent SNP-induced ferritin transcript accumulation.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- suspension culturese0
- Treatment
- SNPe0
- Gene
- ferritine0
- Outcome
- accumulation of ferritin transcripte0
- Direction
- inducee0
- Comparator
- iron chelants DFO or ferrozinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SNP is still able to induce the accumulation of ferritin transcript in Arabidopsis suspension cultures pre-treated with the iron chelants DFO or ferrozine”
Claim · model-written summary
NO scavenging prevented iron-induced ferritin transcript accumulation.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- NO scavenger CPTIOe0
- Gene
- ferritine0
- Outcome
- ferritin transcript accumulatione0
- Direction
- preventse0
- Comparator
- irone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NO scavenger CPTIO prevents ferritin transcript accumulation in Arabidopsis suspension cultures treated with iron”
Claim · model-written summary
The ferritin regulatory pathway depends on serine/threonine phosphatase activity and protein synthesis.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- pathwaye0
- Qualifier
- ser/thr phosphatase-dependent and necessitates protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathway is ser/thr phosphatase-dependent and necessitates protein synthesis”
t0“Arabidopsis”
Claim · model-written summary
NO mediates ferritin regulation through the Atfer1 promoter IDRS sequence.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- Atfer1e0
- Compound
- NOe0
- Outcome
- ferritin regulatione0
- Qualifier
- IDRS sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO mediates ferritin regulation through the IDRS sequence of the Atfer1 promoter responsible for transcriptional repression under low iron supply”
t0“Arabidopsis”
The same questions in other papers
sodium nitroprusside · molecular: expression · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
nitric oxide · molecular: expression · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.