Abstract
The vascular wilt pathogen Fusarium oxysporum f. sp. lycopersici efficiently invades roots and colonizes vascular tissues of its host tomato. For these processes, the F-box protein Frp1 is required. The Fusarium oxysporum Deltafrp1 mutant was characterized in detail to uncover the cause of its colonization defect. Using growth assays, we could attribute poor root colonization to reduced assimilation of organic acids, amino acids (except proline), or polysaccharides, singly or in combination. External root colonization by the Deltafrp1 mutant is restored by the addition of 0.1% glucose or proline but infection still does not occur. This is due to the inability of the Deltafrp1 mutant to penetrate the roots, as demonstrated by the lack of expression of SIX1 in the Deltafrp1 strain, which is a gene exclusively expressed inside roots, and loss of cell wall-degrading enzyme (CWDE) gene expression. Many of the metabolic defects of the Deltafrp1 strain can be attributed to reduced expression of the ICL1 (isocitrate lyase) gene. Strikingly, an Deltaicl1 mutant is still fully pathogenic and capable of external root colonization. We conclude that the inability of the Deltafrp1 strain to colonize and invade roots is not primarily due to metabolic defects but can be attributed to reduced expression of several CWDE genes.
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Claims 10
Claim · model-written summary
Deltafrp1 had poor tomato root colonization associated with reduced nutrient assimilation.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- Deltafrp1e1
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporumt0pathogen - Outcome
- poor root colonizatione0
- Qualifier
- reduced assimilation of organic acids, amino acids (except proline), or polysaccharidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“Using growth assays, we could attribute poor root colonization to reduced assimilation of organic acids, amino acids (except proline), or polysaccharides, singly or in combination.”
t0“Tomato Roots by the Δfrp1 Mutant of Fusarium oxysporum”
e1“External root colonization by the Deltafrp1 mutant is restored by the addition of 0.1% glucose or proline”
Claim · model-written summary
Glucose or proline restored external root colonization by Deltafrp1.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltafrp1e0
- Treatment
- 0.1% glucose or prolinee0
- Outcome
- External root colonizatione0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“External root colonization by the Deltafrp1 mutant is restored by the addition of 0.1% glucose or proline”
t0“Tomato Roots”
Claim · model-written summary
Glucose or proline did not restore infection by Deltafrp1.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltafrp1e0
- Treatment
- 0.1% glucose or prolinee0
- Outcome
- infection still does not occure0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“External root colonization by the Deltafrp1 mutant is restored by the addition of 0.1% glucose or proline but infection still does not occur.”
t0“Tomato Roots”
Claim · model-written summary
Deltafrp1 could not penetrate tomato roots.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltafrp1e0
- Outcome
- inability of the Deltafrp1 mutant to penetrate the rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inability of the Deltafrp1 mutant to penetrate the roots”
t0“Tomato Roots”
Claim · model-written summary
SIX1 expression was absent in Deltafrp1.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltafrp1e0
- Gene
- SIX1e0
- Outcome
- lack of expression of SIX1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lack of expression of SIX1 in the Deltafrp1 strain”
t0“Tomato Roots”
Claim · model-written summary
CWDE gene expression was lost in Deltafrp1.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- cell wall-degrading enzyme (CWDE) genee0
- Outcome
- loss of cell wall-degrading enzyme (CWDE) gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss of cell wall-degrading enzyme (CWDE) gene expression.”
t0“Tomato Roots”
Claim · model-written summary
Reduced ICL1 expression was attributed as a cause of metabolic defects.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltafrp1e0
- Gene
- ICL1 (isocitrate lyase) genee0
- Outcome
- metabolic defects of the Deltafrp1 straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many of the metabolic defects of the Deltafrp1 strain can be attributed to reduced expression of the ICL1 (isocitrate lyase) gene.”
t0“Tomato Roots”
Claim · model-written summary
Deltaicl1 retained full pathogenicity.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltaicl1e0
- Outcome
- fully pathogenice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an Deltaicl1 mutant is still fully pathogenic”
t0“Tomato Roots”
Claim · model-written summary
Deltaicl1 retained external root colonization.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltaicl1e0
- Outcome
- capable of external root colonizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an Deltaicl1 mutant is still fully pathogenic and capable of external root colonization.”
t0“Tomato Roots”
Claim · model-written summary
Reduced CWDE gene expression was attributed as the main cause of Deltafrp1 root invasion defects.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- Deltafrp1e0
- Gene
- CWDE genese0
- Outcome
- inability of the Deltafrp1 strain to colonize and invade rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the inability of the Deltafrp1 strain to colonize and invade roots is not primarily due to metabolic defects but can be attributed to reduced expression of several CWDE genes.”
t0“Tomato Roots”
The same questions in other papers
glucose · disease · Solanum lycopersicum (tomato)Results agree
2 papers in the same direction as this paper
- positive
- positive
proline · disease · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.