Abstract
* High-temperature, low-light (HTLL) treatment of 35S:PAP1 Arabidopsis thaliana over-expressing the PAP1 (Production of Anthocyanin Pigment 1) gene results in reversible reduction of red colouration, suggesting the action of additional anthocyanin regulators. High-performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LCMS) and Affimetrix-based microarrays were used to measure changes in anthocyanin, flavonoids, and gene expression in response to HTLL. * HTLL treatment of control and 35S:PAP1 A. thaliana resulted in a reversible reduction in the concentrations of major anthocyanins despite ongoing over-expression of the PAP1 MYB transcription factor. Twenty-one anthocyanins including eight cis-coumaryl esters were identified by LCMS. The concentrations of nine anthocyanins were reduced and those of three were increased, consistent with a sequential process of anthocyanin degradation. Analysis of gene expression showed down-regulation of flavonol and anthocyanin biosynthesis and of transport-related genes within 24 h of HTLL treatment. No catabolic genes up-regulated by HTLL were found. * Reductions in the concentrations of anthocyanins and down-regulation of the genes of anthocyanin biosynthesis were achieved by environmental manipulation, despite ongoing over-expression of PAP1. Quantitative PCR showed reduced expression of three genes (TT8, TTG1 and EGL3) of the PAP1 transcriptional complex, and increased expression of the potential transcriptional repressors AtMYB3, AtMYB6 and AtMYBL2 coincided with HTLL-induced down-regulation of anthocyanin biosynthesis. * HTLL treatment offers a model system with which to explore anthocyanin catabolism and to discover novel genes involved in the environmental control of anthocyanins.
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Claims 10
Claim · model-written summary
HTLL reversibly reduced red colouration of PAP1 overexpressing Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- 35S:PAP1 Arabidopsis thalianae0
- Treatment
- High-temperature, low-light (HTLL) treatmente0
- Gene
- PAP1e0
- Outcome
- red colouratione0
- Direction
- reductione0
- Qualifier
- reversiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High-temperature, low-light (HTLL) treatment of 35S:PAP1 Arabidopsis thaliana over-expressing the PAP1 (Production of Anthocyanin Pigment 1) gene results in reversible reduction of red colouration”
Claim · model-written summary
HTLL reversibly reduced major anthocyanin concentrations in controls and 35S:PAP1 Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- 35S:PAP1 A. thalianae0
- Treatment
- HTLL treatmente0
- Gene
- PAP1 MYB transcription factore0
- Outcome
- concentrations of major anthocyaninse0
- Direction
- reductione0
- Qualifier
- reversiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HTLL treatment of control and 35S:PAP1 A. thaliana resulted in a reversible reduction in the concentrations of major anthocyanins despite ongoing over-expression of the PAP1 MYB transcription factor”
e1“Arabidopsis thaliana”
Claim · model-written summary
LCMS identified 21 anthocyanins including eight cis-coumaryl esters.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- anthocyanins including eight cis-coumaryl esterse0
- Value
- Twenty-one anthocyaninse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty-one anthocyanins including eight cis-coumaryl esters were identified by LCMS”
e1“Arabidopsis thaliana”
Claim · model-written summary
HTLL reduced concentrations of nine anthocyanins.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HTLLe2
- Outcome
- concentrations of nine anthocyaninse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentrations of nine anthocyanins were reduced and those of three were increased”
e1“Arabidopsis thaliana”
e2“HTLL treatment of control and 35S:PAP1 A. thaliana”
Claim · model-written summary
HTLL increased concentrations of three anthocyanins.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HTLLe2
- Outcome
- those of threee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentrations of nine anthocyanins were reduced and those of three were increased”
e1“Arabidopsis thaliana”
e2“HTLL treatment of control and 35S:PAP1 A. thaliana”
Claim · model-written summary
HTLL downregulated flavonol and anthocyanin biosynthesis genes within 24 hours.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HTLL treatmente0
- Outcome
- flavonol and anthocyanin biosynthesise0
- Direction
- down-regulatione0
- Qualifier
- within 24 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of flavonol and anthocyanin biosynthesis and of transport-related genes within 24 h of HTLL treatment”
e1“Arabidopsis thaliana”
Claim · model-written summary
HTLL downregulated transport related genes within 24 hours.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HTLL treatmente0
- Outcome
- transport-related genese0
- Direction
- down-regulatione0
- Qualifier
- within 24 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of flavonol and anthocyanin biosynthesis and of transport-related genes within 24 h of HTLL treatment”
e1“Arabidopsis thaliana”
Claim · model-written summary
No HTLL upregulated catabolic genes were found.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- catabolic genes up-regulated by HTLLe0
- Direction
- Noe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No catabolic genes up-regulated by HTLL were found”
e1“Arabidopsis thaliana”
Claim · model-written summary
HTLL reduced expression of TT8, TTG1 and EGL3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HTLLe2
- Gene
- TT8, TTG1 and EGL3e0
- Outcome
- expression of three genes (TT8, TTG1 and EGL3)e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced expression of three genes (TT8, TTG1 and EGL3) of the PAP1 transcriptional complex”
e1“Arabidopsis thaliana”
e2“HTLL-induced down-regulation of anthocyanin biosynthesis”
Claim · model-written summary
Increased expression of AtMYB3, AtMYB6 and AtMYBL2 coincided with HTLL induced anthocyanin biosynthesis downregulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- HTLL-inducede0
- Gene
- AtMYB3, AtMYB6 and AtMYBL2e0
- Outcome
- expression of the potential transcriptional repressors AtMYB3, AtMYB6 and AtMYBL2e0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased expression of the potential transcriptional repressors AtMYB3, AtMYB6 and AtMYBL2 coincided with HTLL-induced down-regulation of anthocyanin biosynthesis”
e1“Arabidopsis thaliana”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.